FastQCFastQC Report
Thu 2 Feb 2017
SRR4062839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291692
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49200.38089575533486314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33720.26105294450999156No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21550.16683543755012803No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21120.16350647058277049No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19650.15212604862459472No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19390.15011318487689015No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19230.14887449949368736No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19170.14840999247498632No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18720.1449261898347284No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18410.14252623690477298No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18300.14167464070382105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17760.13749407753551157No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16990.13153290412884805No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16770.12982971172694419No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16510.12781684797923962No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA15980.12371370264738032No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15660.12123633188097473No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15280.11829445409586806No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15280.11829445409586806No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14820.11473323361915998No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14790.11450098010980946No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14690.1137268017453077No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14670.11357196607240737No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14640.11333971256305682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14140.10946882074054805No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13490.10443666137128664No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13480.10435924353483648No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13130.10164961925908034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC4550.019.33986330
CTAGCGG4850.018.14288129
TCTAGCG4850.018.1421828
GGTCTAC802.7659244E-618.0208871
ATCGTTT4150.016.962529
AAGACGG4450.016.8968735
CGTTTAT3900.016.81982231
CATCGTT4100.016.77849828
TAGGACG1850.016.4298484
ACTATAC703.697997E-415.9993433
CGTCGTA4100.015.99934210
ATCCCGT1203.112291E-815.99934120
TAGACTG600.002444058715.9981035
ATACCGT4200.015.9981026
CAAGACG4900.015.9974844
AGCGGCG5700.015.71865331
ACCGTCG4350.015.44644458
TCGTTTA4250.015.4346630
CCCGTTT1255.140646E-815.35936822
GATATAC2100.015.2557771
Produced by FastQC (version 0.11.5)