##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062839_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1291692
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18748509706648	32.0	32.0	32.0	32.0	32.0
2	30.75971129340431	32.0	32.0	32.0	32.0	32.0
3	30.82325507938425	32.0	32.0	32.0	32.0	32.0
4	30.89722782211239	32.0	32.0	32.0	32.0	32.0
5	30.78483647804585	32.0	32.0	32.0	32.0	32.0
6	34.44525475113262	36.0	36.0	36.0	32.0	36.0
7	34.34975752733624	36.0	36.0	36.0	32.0	36.0
8	34.32224400244021	36.0	36.0	36.0	32.0	36.0
9	34.487569792179556	36.0	36.0	36.0	32.0	36.0
10	34.166813760555925	36.0	36.0	36.0	32.0	36.0
11	34.49268633699055	36.0	36.0	36.0	32.0	36.0
12	34.27177531485834	36.0	36.0	36.0	32.0	36.0
13	34.39364182792802	36.0	36.0	36.0	32.0	36.0
14	34.27007134827807	36.0	36.0	36.0	32.0	36.0
15	34.21605692378679	36.0	36.0	36.0	32.0	36.0
16	34.23207235161323	36.0	36.0	36.0	32.0	36.0
17	34.12880005450216	36.0	36.0	36.0	32.0	36.0
18	34.17157186078415	36.0	36.0	36.0	32.0	36.0
19	34.15514147335433	36.0	36.0	36.0	32.0	36.0
20	34.13030660559948	36.0	36.0	36.0	32.0	36.0
21	34.11367493179488	36.0	36.0	36.0	32.0	36.0
22	34.089131929283454	36.0	36.0	36.0	32.0	36.0
23	34.03528937238908	36.0	36.0	36.0	32.0	36.0
24	34.020668239797104	36.0	36.0	36.0	32.0	36.0
25	34.001135719660724	36.0	36.0	36.0	32.0	36.0
26	33.9569425219015	36.0	36.0	36.0	32.0	36.0
27	33.97404412197335	36.0	36.0	36.0	32.0	36.0
28	33.94890190540779	36.0	36.0	36.0	32.0	36.0
29	33.91398955788222	36.0	36.0	36.0	32.0	36.0
30	33.88614391046782	36.0	36.0	36.0	32.0	36.0
31	33.899735385835015	36.0	36.0	36.0	32.0	36.0
32	33.84921947337291	36.0	36.0	36.0	32.0	36.0
33	33.796204513150194	36.0	36.0	36.0	32.0	36.0
34	33.806633469898394	36.0	36.0	36.0	32.0	36.0
35	33.760714628564706	36.0	36.0	36.0	32.0	36.0
36	33.720065619358174	36.0	36.0	36.0	27.0	36.0
37	33.7244312111556	36.0	36.0	36.0	27.0	36.0
38	33.09651139745388	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	2.0
9	3.0
10	14.0
11	16.0
12	7.0
13	7.0
14	138.0
15	358.0
16	446.0
17	534.0
18	702.0
19	926.0
20	1136.0
21	1769.0
22	2737.0
23	4135.0
24	6208.0
25	9330.0
26	13590.0
27	19141.0
28	27227.0
29	36122.0
30	49290.0
31	65844.0
32	89578.0
33	133443.0
34	289606.0
35	539379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.81496602225083	17.40868407243109	11.391673106690984	25.384676798627094
2	17.02194078119251	19.63779314084316	37.09150061000407	26.24876546796026
3	18.895847834721724	24.06253605183877	28.364367272324653	28.677248841114857
4	12.344570285856737	15.222647428388719	35.793137673120114	36.639644612634434
5	14.753485361790153	36.57965391023219	33.03034478914413	15.636515938833526
6	34.344282030299816	35.67176669962088	16.65615073574098	13.327800534338321
7	30.50396728010027	30.480509657495485	20.644178832243934	18.371344230160307
8	28.832968952254724	31.967007512650113	19.29150073392336	19.9085228011718
9	27.928007438323	13.801077503950253	18.64380667482653	39.62710838290022
10	16.488759652113593	25.864799959123193	30.64932189402068	26.997118494742526
11	37.88191046587967	21.09232741641789	22.036613236878942	18.989148880823496
12	25.38836075181532	23.855589027032927	27.91358629718491	22.842463923966843
13	29.503271749137248	18.9661566955295	25.434936287805527	26.095635267527722
14	24.10264113078057	20.023645677192373	24.679304535262563	31.194408656764487
15	25.367233125860487	27.069940390920337	22.399168663186245	25.163657820032924
16	26.17337785867977	25.486141060075923	23.518116618107403	24.8223644631369
17	24.02889485466235	25.668691020340844	24.95839918354768	25.344014941449128
18	25.37751158238981	24.22476959096202	26.33081349255506	24.066905334093107
19	25.812603070633873	24.551521767743633	25.456530323090142	24.17934483853235
20	26.051975119578696	23.68045350869994	24.824367576171902	25.443203795549458
21	27.915986633674102	23.514250497830606	24.23831143291597	24.331451435579325
22	26.437983895943017	23.69216475689068	24.9870703004026	24.882781046763704
23	24.526814734980636	23.596626111247897	25.9096321829469	25.966926970824566
24	25.135790348219487	24.803544370052798	25.21174777179943	24.848917509928278
25	25.023403400287574	24.28719321026513	25.32499560581318	25.364407783634114
26	24.626933857804346	25.594753225032136	25.855388475059236	23.922924442104286
27	25.52596472999329	24.378299740085584	24.99150264908302	25.104232880838108
28	24.435415252091765	24.521431729163456	26.01669385489307	25.02645916385171
29	24.34392987052806	24.72711168096727	25.88494170588299	25.04401674262168
30	24.198487052617153	25.055761966661272	26.104806948626212	24.64094403209536
31	25.283502101635708	24.93184878133001	24.42202114720121	25.362627969833067
32	24.678287866646535	24.762911261569904	24.42542418154157	26.133376690241988
33	24.42549270857125	24.300684817491685	25.344828253657326	25.928994220279733
34	25.56349702253059	24.48299811154651	25.431560788613222	24.521944077309676
35	26.134805343029726	24.28653394524254	25.453569827414658	24.125090884313078
36	24.362820970443195	25.373728332583568	24.73726551726541	25.526185179707834
37	25.7063485427061	25.192401197920056	24.683176779923997	24.41807347944985
38	24.54924404308428	24.789100098481892	25.324169562282055	25.337486296151774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	75.0
1	132.0
2	189.0
3	189.0
4	477.5
5	766.0
6	766.0
7	1037.0
8	1308.0
9	1413.5
10	1519.0
11	1519.0
12	1961.5
13	2404.0
14	3101.5
15	3799.0
16	3799.0
17	5507.0
18	7215.0
19	7215.0
20	8421.0
21	9627.0
22	9872.5
23	10118.0
24	10118.0
25	11671.5
26	13225.0
27	13225.0
28	17034.0
29	20843.0
30	25500.5
31	30158.0
32	30158.0
33	37828.0
34	45498.0
35	45498.0
36	49712.0
37	53926.0
38	61478.5
39	69031.0
40	69031.0
41	72235.5
42	75440.0
43	86798.0
44	98156.0
45	98156.0
46	98000.0
47	97844.0
48	97844.0
49	105265.5
50	112687.0
51	115426.0
52	118165.0
53	118165.0
54	113188.0
55	108211.0
56	108211.0
57	106095.5
58	103980.0
59	93187.0
60	82394.0
61	82394.0
62	79914.5
63	77435.0
64	64761.0
65	52087.0
66	52087.0
67	44074.0
68	36061.0
69	36061.0
70	29174.5
71	22288.0
72	17374.0
73	12460.0
74	12460.0
75	9569.0
76	6678.0
77	6678.0
78	6539.0
79	6400.0
80	5100.5
81	3801.0
82	3801.0
83	3816.5
84	3832.0
85	3832.0
86	2605.5
87	1379.0
88	1164.0
89	949.0
90	949.0
91	702.0
92	455.0
93	402.0
94	349.0
95	349.0
96	318.5
97	288.0
98	288.0
99	470.0
100	652.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1225524351006277
2	0.055508588734775786
3	0.01153525763107614
4	0.0023999529299554385
5	3.096713458007017E-4
6	7.741783645017542E-5
7	7.741783645017542E-5
8	3.096713458007017E-4
9	2.3225350935052628E-4
10	4.6450701870105255E-4
11	0.002167699420604912
12	0.0034838026402578943
13	0.014012628397481753
14	0.009599811719821754
15	0.021522158533148768
16	0.011070750612375087
17	0.019431876948994033
18	0.005883755570213333
19	0.007896619317917894
20	0.005728919897312982
21	0.006812769607615438
22	0.007122440953416139
23	0.009754647392722103
24	0.0138577927245814
25	0.016489999163887367
26	0.017960938056440702
27	0.008748215518869824
28	0.006116009079563859
29	0.01068366143012421
30	0.0031741312944571927
31	0.006116009079563859
32	0.0067353517711652615
33	0.008283708500168772
34	0.012309435995577893
35	0.016644834836787716
36	0.0138577927245814
37	0.009290140374021053
38	0.006503098261814736
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1291692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.58223425171268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85864546680259	36.095238386190225
2	14.01708979749312	13.339289005432192
3	4.722496691967333	6.741208315503836
4	1.9712855113440242	3.7519267591111425
5	1.0068623933281087	2.395438112928907
6	0.5618557916040516	1.604061233507127
7	0.3591265429136422	1.196163030364727
8	0.24335899344654363	0.9263651706747559
9	0.17665450578555134	0.7565054468317773
>10	0.8487753850170884	7.325011191659506
>50	0.0967969395909412	3.2509652832406637
>100	0.11274157620147786	11.61743159385324
>500	0.01696833603076483	5.45812072513813
>1k	0.007342068474850166	5.542275745563837
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4920	0.38089575533486314	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3372	0.26105294450999156	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2155	0.16683543755012803	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2112	0.16350647058277049	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1965	0.15212604862459472	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1939	0.15011318487689015	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1923	0.14887449949368736	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1917	0.14840999247498632	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1872	0.1449261898347284	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1841	0.14252623690477298	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1830	0.14167464070382105	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1776	0.13749407753551157	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1699	0.13153290412884805	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1677	0.12982971172694419	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1651	0.12781684797923962	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1598	0.12371370264738032	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1566	0.12123633188097473	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1528	0.11829445409586806	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1528	0.11829445409586806	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1482	0.11473323361915998	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1479	0.11450098010980946	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1469	0.1137268017453077	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1467	0.11357196607240737	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1464	0.11333971256305682	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1414	0.10946882074054805	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1349	0.10443666137128664	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1348	0.10435924353483648	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1313	0.10164961925908034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5483567290035087E-4
2	0.0	0.0	0.0	0.0	1.5483567290035087E-4
3	0.0	0.0	0.0	0.0	1.5483567290035087E-4
4	0.0	0.0	0.0	0.0	1.5483567290035087E-4
5	0.0	0.0	0.0	0.0	1.5483567290035087E-4
6	0.0	0.0	0.0	0.0	1.5483567290035087E-4
7	0.0	0.0	0.0	0.0	1.5483567290035087E-4
8	0.0	0.0	0.0	7.741783645017543E-5	1.5483567290035087E-4
9	0.0	0.0	0.0	7.741783645017543E-5	1.5483567290035087E-4
10	0.0	0.0	0.0	7.741783645017543E-5	1.5483567290035087E-4
11	0.0	0.0	0.0	7.741783645017543E-5	1.5483567290035087E-4
12	0.0	0.0	0.0	7.741783645017543E-5	4.6450701870105255E-4
13	0.0	0.0	0.0	7.741783645017543E-5	6.967605280515789E-4
14	0.0	0.0	0.0	7.741783645017543E-5	6.967605280515789E-4
15	0.0	0.0	0.0	7.741783645017543E-5	0.001083849710302456
16	0.0	0.0	0.0	7.741783645017543E-5	0.0011612675467526313
17	0.0	0.0	0.0	7.741783645017543E-5	0.0011612675467526313
18	0.0	0.0	0.0	3.0967134580070174E-4	0.001238685383202807
19	0.0	0.0	0.0	3.8708918225087717E-4	0.001238685383202807
20	0.0	0.0	0.0	4.6450701870105255E-4	0.0015483567290035087
21	0.0	0.0	0.0	6.193426916014035E-4	0.001625774565453684
22	0.0	0.0	7.741783645017543E-5	8.515962009519297E-4	0.0017031924019038594
23	0.0	0.0	7.741783645017543E-5	0.001470938892553333	0.0017031924019038594
24	0.0	0.0	7.741783645017543E-5	0.0023225350935052627	0.0017031924019038594
25	0.0	0.0	7.741783645017543E-5	0.0026322064393059647	0.0017031924019038594
26	0.0	0.0	7.741783645017543E-5	0.0030967134580070174	0.0017806102383540348
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	455	0.0	19.339863	30
CTAGCGG	485	0.0	18.142881	29
TCTAGCG	485	0.0	18.14218	28
GGTCTAC	80	2.7659244E-6	18.020887	1
ATCGTTT	415	0.0	16.9625	29
AAGACGG	445	0.0	16.896873	5
CGTTTAT	390	0.0	16.819822	31
CATCGTT	410	0.0	16.778498	28
TAGGACG	185	0.0	16.429848	4
ACTATAC	70	3.697997E-4	15.999343	3
CGTCGTA	410	0.0	15.999342	10
ATCCCGT	120	3.112291E-8	15.999341	20
TAGACTG	60	0.0024440587	15.998103	5
ATACCGT	420	0.0	15.998102	6
CAAGACG	490	0.0	15.997484	4
AGCGGCG	570	0.0	15.718653	31
ACCGTCG	435	0.0	15.4464445	8
TCGTTTA	425	0.0	15.43466	30
CCCGTTT	125	5.140646E-8	15.359368	22
GATATAC	210	0.0	15.255777	1
>>END_MODULE