FastQCFastQC Report
Thu 2 Feb 2017
SRR4062839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291692
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54900.4250239221114631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT42180.3265484341468399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23090.17875778436345507No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22930.17751909898025225No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22160.17155792557358876No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21500.16644834836787717No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20680.1601000857789628No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20510.1587839825593098No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20350.157545297176107No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19550.15135187026009297No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18740.14508102550762877No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18450.14283590825057368No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.13416511056815403No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17200.13315867869430173No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16980.13145548629239787No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16810.1301393830727449No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16620.12866844418019155No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16470.12750717663343894No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16280.1260362377408856No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15400.11922346813327016No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15140.11721060438556559No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14390.11140426665180243No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14180.10977849208634875No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14130.10939140290409789No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14070.10892689588539682No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13950.10799788184799473No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG13420.10389473651613541No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC13380.10358506517033472No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13370.10350764733388455No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13370.10350764733388455No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC13030.10087544089457859No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG12960.10033351603942736No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12940.10017868036652701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT707.7877667E-720.5709081
GGACCGT659.427909E-619.6940946
AACGAAT1208.185452E-1118.66619131
GTATTAC701.7879292E-518.2852521
ATCGTTT3400.017.88189929
CATCGTT3400.017.88189928
GAATACG450.00885214617.77732723
CTAGCGG3700.017.7292829
TCTATAC652.0998227E-417.2316653
TAGCGGC3900.017.23033130
CGCGGTC2850.016.84232910
TACCGTC4850.016.826277
TCTAGCG4050.016.5921728
ACCGTCG4850.016.1664168
ATACCGT5150.016.1568056
AACCGCG2900.016.0014517
AGAACCG3000.016.0014515
CCGTCGT4800.016.0014519
CGTCGTA4900.016.00021410
GTGTTAA1001.3150238E-615.9995941
Produced by FastQC (version 0.11.5)