FastQCFastQC Report
Thu 2 Feb 2017
SRR4062838_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062838_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890330
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25010.2809070793975268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16880.18959262296002605No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9620.1080498242224793No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9490.10658969146271607No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9450.10614041984432739No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9380.10535419451214718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9020.10131074994664899No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8980.10086147832826031No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8930.10029988880527446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG350.0020662422.8570219
TGAACCG400.00448866919.9954015
GTATAGA802.7680126E-618.0181241
TCTAGCG1600.017.99788328
TAGCCCG450.00886047617.7736915
ATACTTA550.001366936817.4495536
TAGGACG1850.017.2933224
GTGTTAC652.0836452E-417.248121
CCGATAA1404.0017767E-1117.1427679
GATAACG1505.456968E-1217.06753511
CGATAAC1456.91216E-1116.55256710
ATAACGA1551.0913936E-1116.5169712
CTAGCGG1750.016.45705629
GGACGTG2050.016.3855556
TTCCGAT1601.8189894E-1115.9981177
TTACTAC908.590601E-615.997223
TAATACC703.7017037E-415.9963224
ATATACT1001.31671E-615.9963214
CCTATTC2750.015.7063613
ACTCTAA2550.015.68707310
Produced by FastQC (version 0.11.5)