##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062838_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	890330
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263492188289735	32.0	32.0	32.0	32.0	32.0
2	30.98625116529826	32.0	32.0	32.0	32.0	32.0
3	31.023221726775464	32.0	32.0	32.0	32.0	32.0
4	31.058713061449126	32.0	32.0	32.0	32.0	32.0
5	31.040121078701155	32.0	32.0	32.0	32.0	32.0
6	34.63710421978367	36.0	36.0	36.0	32.0	36.0
7	34.588397560455114	36.0	36.0	36.0	32.0	36.0
8	34.56841171251109	36.0	36.0	36.0	32.0	36.0
9	34.67435108330619	36.0	36.0	36.0	32.0	36.0
10	34.44159693596756	36.0	36.0	36.0	32.0	36.0
11	34.665239854885264	36.0	36.0	36.0	32.0	36.0
12	34.51941864252581	36.0	36.0	36.0	32.0	36.0
13	34.5882043736592	36.0	36.0	36.0	32.0	36.0
14	34.485291970393	36.0	36.0	36.0	32.0	36.0
15	34.45903766019341	36.0	36.0	36.0	32.0	36.0
16	34.47819010928532	36.0	36.0	36.0	32.0	36.0
17	34.40598205159885	36.0	36.0	36.0	32.0	36.0
18	34.441275706760415	36.0	36.0	36.0	32.0	36.0
19	34.39987420394685	36.0	36.0	36.0	32.0	36.0
20	34.38755629934968	36.0	36.0	36.0	32.0	36.0
21	34.358367122302965	36.0	36.0	36.0	32.0	36.0
22	34.33279682814237	36.0	36.0	36.0	32.0	36.0
23	34.296090213740975	36.0	36.0	36.0	32.0	36.0
24	34.28662630709962	36.0	36.0	36.0	32.0	36.0
25	34.269798838632866	36.0	36.0	36.0	32.0	36.0
26	34.22637112082037	36.0	36.0	36.0	32.0	36.0
27	34.22508171127559	36.0	36.0	36.0	32.0	36.0
28	34.20466456257792	36.0	36.0	36.0	32.0	36.0
29	34.173601922882526	36.0	36.0	36.0	32.0	36.0
30	34.148385430121415	36.0	36.0	36.0	32.0	36.0
31	34.160621342648234	36.0	36.0	36.0	32.0	36.0
32	34.11665225253558	36.0	36.0	36.0	32.0	36.0
33	34.07810362449878	36.0	36.0	36.0	32.0	36.0
34	34.07831590533847	36.0	36.0	36.0	32.0	36.0
35	34.03866319229949	36.0	36.0	36.0	32.0	36.0
36	33.98836948097896	36.0	36.0	36.0	32.0	36.0
37	33.99662821650399	36.0	36.0	36.0	32.0	36.0
38	33.33521840216549	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	5.0
11	16.0
12	2.0
13	2.0
14	218.0
15	406.0
16	497.0
17	563.0
18	648.0
19	879.0
20	1019.0
21	1329.0
22	1824.0
23	2415.0
24	3213.0
25	4617.0
26	6874.0
27	9711.0
28	13937.0
29	19232.0
30	27294.0
31	38133.0
32	55116.0
33	86908.0
34	202060.0
35	413410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68360508804898	17.87710816546168	11.623769569094748	25.815517177394597
2	16.30915709255765	20.29145449661895	37.91054974472164	25.488838666101753
3	18.347489185123944	24.68117037684409	29.25447844270278	27.71686199532919
4	12.407770370827976	15.907694554113478	36.77470080478039	34.90983427027816
5	14.272492834101588	36.79554051989828	33.98612646125922	14.945840184740906
6	33.05235042855041	36.29565317012738	17.30016050282649	13.35183589849572
7	29.17906843529927	31.13351229319466	21.66556220727146	18.02185706423461
8	27.659020046544647	33.02277362949385	20.107084130792245	19.21112219316926
9	27.41826064492941	14.503723338537396	19.269596666404592	38.8084193501286
10	15.80642190293914	26.714312028825333	32.10973933202146	25.36952673621407
11	36.56937536784692	21.53291640271544	22.90220550905521	18.995502720382426
12	24.46128254347648	24.148132839272655	29.261301413329527	22.129283203921336
13	29.2940912154572	19.72972365760503	25.68321725454954	25.29296787238823
14	23.42585114401349	20.289067142342034	25.661083546851813	30.62399816679266
15	24.857181054540266	27.48145460183145	22.95686053343789	24.704503810190392
16	25.19155312769946	26.12075850657644	24.286499000812157	24.40118936491194
17	23.55907659573034	26.2405618885098	25.763221781717725	24.437139734042134
18	24.36480943159711	25.069640698923042	26.98475991930665	23.5807899501732
19	25.209572430680755	25.424227617619472	25.977770663791116	23.388429287908657
20	25.267642621982244	24.75577716048897	25.65527652753706	24.321303689991723
21	26.49040022195239	24.644690833568276	24.888658749316228	23.9762501951631
22	25.29095204023063	24.85119632649531	25.35385451780196	24.5039971154721
23	23.839109097179083	24.61844687349409	26.070073002539758	25.472371026787066
24	24.23337624494216	25.585316591065848	25.72303814190487	24.458269022087123
25	24.409349748304525	24.967000425764716	25.817631152946145	24.80601867298461
26	24.144307289958164	25.711785349983597	26.219898534638876	23.924008825419367
27	24.82162434176237	25.427742132600063	25.284187918980606	24.46644560665696
28	23.925565158006115	25.157759002203793	26.20192253690404	24.714753302886052
29	23.989454493578677	25.513655352451085	26.104621578298815	24.392268575671423
30	24.061614770651886	25.50763445377207	26.347682706844612	24.083068068731425
31	24.63862858015768	25.134984257920745	25.24932915042194	24.977058011499636
32	24.046026176152115	25.632500482996285	25.22251526492908	25.09895807592252
33	23.900257679269064	25.0893565207233	25.87632293704943	25.134062862958213
34	24.54334578336937	25.18632929877278	26.08407986744924	24.186245050408605
35	25.345491146754256	25.10419217643134	25.77360584689423	23.776710829920173
36	24.099830036857174	25.766210248788745	25.445156275099894	24.688803439254183
37	24.84771080385556	25.62996561628402	25.331059806997384	24.191263772863035
38	23.98523612207831	25.545979303163836	25.89249910983161	24.576285464926244
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	628.0
1	452.0
2	276.0
3	276.0
4	562.5
5	849.0
6	849.0
7	973.5
8	1098.0
9	1020.0
10	942.0
11	942.0
12	1175.5
13	1409.0
14	1881.5
15	2354.0
16	2354.0
17	3496.0
18	4638.0
19	4638.0
20	5636.5
21	6635.0
22	7313.5
23	7992.0
24	7992.0
25	9500.5
26	11009.0
27	11009.0
28	13929.5
29	16850.0
30	20406.0
31	23962.0
32	23962.0
33	29745.5
34	35529.0
35	35529.0
36	38937.0
37	42345.0
38	47648.5
39	52952.0
40	52952.0
41	55733.5
42	58515.0
43	64810.0
44	71105.0
45	71105.0
46	73009.5
47	74914.0
48	74914.0
49	78186.5
50	81459.0
51	81098.5
52	80738.0
53	80738.0
54	76058.0
55	71378.0
56	71378.0
57	68429.0
58	65480.0
59	58615.5
60	51751.0
61	51751.0
62	48610.5
63	45470.0
64	37231.0
65	28992.0
66	28992.0
67	24195.0
68	19398.0
69	19398.0
70	15718.5
71	12039.0
72	9391.5
73	6744.0
74	6744.0
75	5149.5
76	3555.0
77	3555.0
78	3536.5
79	3518.0
80	2769.5
81	2021.0
82	2021.0
83	2029.0
84	2037.0
85	2037.0
86	1331.5
87	626.0
88	519.0
89	412.0
90	412.0
91	292.5
92	173.0
93	144.0
94	115.0
95	115.0
96	103.5
97	92.0
98	92.0
99	211.0
100	330.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12456055619826356
2	0.05806835667673784
3	0.013590466456257793
4	0.0028079476149292957
5	2.2463580919434366E-4
6	3.369537137915155E-4
7	0.0
8	2.2463580919434366E-4
9	0.0
10	2.2463580919434366E-4
11	0.0015724506643604059
12	0.0023586759965406085
13	0.014601327597632338
14	0.00977165769995395
15	0.02437298529758629
16	0.012018015791897386
17	0.021228083968865476
18	0.005166623611469905
19	0.007300663798816169
20	0.006402120562038795
21	0.00550357732526142
22	0.007076027989621826
23	0.00977165769995395
24	0.014376691788437994
25	0.0187570900677277
26	0.0195433153999079
27	0.008311524940190716
28	0.005391259420664248
29	0.01044556512753698
30	0.002695629710332124
31	0.004605034088484045
32	0.006065166848247279
33	0.008536160749385058
34	0.011793379982703042
35	0.016623049880381432
36	0.015387552929812542
37	0.009322386081565262
38	0.00550357732526142
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	890330.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.99023874364419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.86716852282926	54.08793445493745
2	9.215103102138835	11.609230889020427
3	2.2043126299119353	4.165505364713491
4	0.876331743666837	2.2080138300883227
5	0.44831287123599806	1.4119667395502065
6	0.2670259344865225	1.009201641843045
7	0.1749176140592475	0.7712671589042429
8	0.12293164117104848	0.6194794741209875
9	0.09619382987806852	0.5453345078716526
>10	0.5674997708925366	6.949829965770736
>50	0.07384551208113804	3.3268842728469803
>100	0.07956270874363228	9.867028827873801
>500	0.006436533699565017	2.95654978639945
>1k	3.5758520553138987E-4	0.4717730860592449
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2501	0.2809070793975268	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1688	0.18959262296002605	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	962	0.1080498242224793	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	949	0.10658969146271607	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	945	0.10614041984432739	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	938	0.10535419451214718	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	902	0.10131074994664899	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	898	0.10086147832826031	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	893	0.10029988880527446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.1231790459717183E-4	0.0	0.0	0.0	0.0
7	1.1231790459717183E-4	0.0	0.0	0.0	0.0
8	1.1231790459717183E-4	0.0	0.0	0.0	0.0
9	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4	0.0
10	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4	0.0
11	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4	0.0
12	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4	1.1231790459717183E-4
13	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4	2.2463580919434366E-4
14	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4	2.2463580919434366E-4
15	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4	3.369537137915155E-4
16	1.1231790459717183E-4	1.1231790459717183E-4	0.0	2.2463580919434366E-4	3.369537137915155E-4
17	1.1231790459717183E-4	1.1231790459717183E-4	0.0	2.2463580919434366E-4	3.369537137915155E-4
18	1.1231790459717183E-4	1.1231790459717183E-4	0.0	3.369537137915155E-4	3.369537137915155E-4
19	1.1231790459717183E-4	1.1231790459717183E-4	0.0	3.369537137915155E-4	4.492716183886873E-4
20	1.1231790459717183E-4	1.1231790459717183E-4	0.0	5.615895229858592E-4	4.492716183886873E-4
21	1.1231790459717183E-4	1.1231790459717183E-4	0.0	7.862253321802028E-4	5.615895229858592E-4
22	1.1231790459717183E-4	1.1231790459717183E-4	0.0	0.001347814855166062	5.615895229858592E-4
23	1.1231790459717183E-4	1.1231790459717183E-4	0.0	0.0017970864735547493	5.615895229858592E-4
24	1.1231790459717183E-4	1.1231790459717183E-4	0.0	0.002920265519526468	5.615895229858592E-4
25	1.1231790459717183E-4	1.1231790459717183E-4	0.0	0.003481855042512327	5.615895229858592E-4
26	1.1231790459717183E-4	1.1231790459717183E-4	0.0	0.004605034088484046	5.615895229858592E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTACG	35	0.00206624	22.857021	9
TGAACCG	40	0.004488669	19.995401	5
GTATAGA	80	2.7680126E-6	18.018124	1
TCTAGCG	160	0.0	17.997883	28
TAGCCCG	45	0.008860476	17.773691	5
ATACTTA	55	0.0013669368	17.449553	6
TAGGACG	185	0.0	17.293322	4
GTGTTAC	65	2.0836452E-4	17.24812	1
CCGATAA	140	4.0017767E-11	17.142767	9
GATAACG	150	5.456968E-12	17.067535	11
CGATAAC	145	6.91216E-11	16.552567	10
ATAACGA	155	1.0913936E-11	16.51697	12
CTAGCGG	175	0.0	16.457056	29
GGACGTG	205	0.0	16.385555	6
TTCCGAT	160	1.8189894E-11	15.998117	7
TTACTAC	90	8.590601E-6	15.99722	3
TAATACC	70	3.7017037E-4	15.996322	4
ATATACT	100	1.31671E-6	15.996321	4
CCTATTC	275	0.0	15.706361	3
ACTCTAA	255	0.0	15.687073	10
>>END_MODULE