FastQCFastQC Report
Thu 2 Feb 2017
SRR4062838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890330
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28660.3219031145754945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21270.23890018307818447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11380.12781777543158154No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10970.1232127413430975No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10400.11681062078105871No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9620.1080498242224793No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9340.10490492289375848No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9320.10468028708456416No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9310.10456796917996697No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9050.1016477036604405No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.10108611413745465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG350.002063100222.8630035
TACCGTC1750.020.1194427
CGTGAGA400.00448479519.99838612
ATACCGT1800.019.5605686
ACCGTCG1650.019.398918
CCGTCGT1750.018.2893739
CGTCGTA1850.018.16375510
GCGACGC450.00885286817.77634232
TCCTATA652.0992261E-417.2313142
ATCTAGG753.2330507E-517.0662461
TCTAGAT1800.016.8894212
GTGTAGG1001.3138924E-615.9996061
AGGGGCG2100.015.99870832
GCTCGTT600.00244264115.99870812
TTTCGTT2650.015.69684628
GTCGTAG2050.015.610249511
GTCCTAC1357.903509E-915.4070281
TAATACT1255.119182E-815.3630744
TCGTTCT2650.015.09312130
TTCGTTC2650.015.09312129
Produced by FastQC (version 0.11.5)