##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	890330
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.160692102928127	32.0	32.0	32.0	32.0	32.0
2	31.20075814585603	32.0	32.0	32.0	32.0	32.0
3	31.317126234092978	32.0	32.0	32.0	32.0	32.0
4	31.43596306987297	32.0	32.0	32.0	32.0	32.0
5	31.32879157166444	32.0	32.0	32.0	32.0	32.0
6	34.86749744476767	36.0	36.0	36.0	32.0	36.0
7	34.90494423416037	36.0	36.0	36.0	36.0	36.0
8	34.845680814978714	36.0	36.0	36.0	32.0	36.0
9	34.978567497444764	36.0	36.0	36.0	36.0	36.0
10	34.79385845697663	36.0	36.0	36.0	32.0	36.0
11	34.98087787674233	36.0	36.0	36.0	36.0	36.0
12	34.8797019082812	36.0	36.0	36.0	32.0	36.0
13	34.94268192692597	36.0	36.0	36.0	36.0	36.0
14	34.88434063774106	36.0	36.0	36.0	32.0	36.0
15	34.857431514157675	36.0	36.0	36.0	32.0	36.0
16	34.86324733525771	36.0	36.0	36.0	32.0	36.0
17	34.83205552997203	36.0	36.0	36.0	32.0	36.0
18	34.832177956488046	36.0	36.0	36.0	32.0	36.0
19	34.83113789269147	36.0	36.0	36.0	32.0	36.0
20	34.81229207147911	36.0	36.0	36.0	32.0	36.0
21	34.80652454707805	36.0	36.0	36.0	32.0	36.0
22	34.78110138937248	36.0	36.0	36.0	32.0	36.0
23	34.73749508609168	36.0	36.0	36.0	32.0	36.0
24	34.70652791661519	36.0	36.0	36.0	32.0	36.0
25	34.68952298585918	36.0	36.0	36.0	32.0	36.0
26	34.62574214055463	36.0	36.0	36.0	32.0	36.0
27	34.608221670616516	36.0	36.0	36.0	32.0	36.0
28	34.58236608897825	36.0	36.0	36.0	32.0	36.0
29	34.53556771084879	36.0	36.0	36.0	32.0	36.0
30	34.52174811586715	36.0	36.0	36.0	32.0	36.0
31	34.506963710085024	36.0	36.0	36.0	32.0	36.0
32	34.47847315040491	36.0	36.0	36.0	32.0	36.0
33	34.43554187773073	36.0	36.0	36.0	32.0	36.0
34	34.43053137600665	36.0	36.0	36.0	32.0	36.0
35	34.41082744600317	36.0	36.0	36.0	32.0	36.0
36	34.3698460121528	36.0	36.0	36.0	32.0	36.0
37	34.358935450900226	36.0	36.0	36.0	32.0	36.0
38	33.910145676322266	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	17.0
22	47.0
23	169.0
24	505.0
25	1268.0
26	2818.0
27	5592.0
28	10051.0
29	16212.0
30	25486.0
31	37070.0
32	54623.0
33	89318.0
34	213997.0
35	433146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96949392810326	19.25259112899874	12.417649444144278	26.36026549875372
2	15.382049352487279	21.355340154774073	37.02761897274044	26.234991519998204
3	17.342221423517124	25.393393460851595	29.709096627093324	27.55528848853796
4	12.02634082457159	16.78548042352883	36.77528194633669	34.41289680556289
5	13.81566385497512	37.42230408949491	33.52172789864432	15.240304156885648
6	33.0182067323352	36.806352700684016	16.9182213336628	13.257219233317983
7	29.058555153579096	31.344579439147363	21.522076819311643	18.074788587961894
8	27.47436286237378	33.4593568532309	19.896103604362526	19.170176680032796
9	27.401745498657743	14.140560940817037	19.002235226723876	39.455458333801346
10	15.610886410631672	27.067428489275215	32.002390149170914	25.319294950922195
11	36.760345059981134	21.443927753066454	22.791481331715865	19.004245855236555
12	24.269258071182755	24.397750042680897	29.086246866323425	22.246745019812924
13	29.199655408281654	19.721473747345083	25.656807764320828	25.422063080052432
14	23.32135275684297	20.091651410151293	25.75629261060506	30.830703222400686
15	24.761155975874114	27.6011141936136	22.873878224927836	24.763851605584446
16	25.163141756427393	26.207585951276492	24.175305785495265	24.45396650680085
17	23.60731414194737	26.21084317050981	25.784259768849754	24.397582918693068
18	24.456100546988196	25.006009007895948	26.970112205586695	23.567778239529165
19	25.160895398335448	25.43248009165141	26.025406310019882	23.381218199993263
20	25.293767479473907	24.568081497871574	25.801332090348524	24.336818932306
21	26.394145990812394	24.712185369469747	24.93727045028248	23.956398189435376
22	25.15207878444798	24.856008122849108	25.49678320798627	24.495129884716643
23	23.75823714208375	24.507175453513145	26.15263830030989	25.581949104093216
24	24.399155369357427	25.52312064066133	25.69822425392832	24.379499736052924
25	24.430716700549233	24.734985904102974	25.944986690328303	24.889310705019486
26	24.14857412420114	25.51918951400043	26.310918423505893	24.021317938292544
27	24.867436644206805	25.224271027900926	25.374552553045	24.533739774847277
28	24.021991845720127	24.972089000707605	26.28362517268878	24.722293980883492
29	24.120494648051846	25.269506812080916	26.121325800545865	24.488672739321377
30	24.16890366493323	25.238844024125882	26.361349162670024	24.230903148270865
31	24.617389057989733	25.0158929835005	25.399121673986052	24.967596284523715
32	24.08578841553132	25.38081385553671	25.400918760459607	25.132478968472366
33	23.879460424786313	24.9125605112711	26.045511214942774	25.16246784899981
34	24.538766524771713	24.921096672020486	26.367189693709076	24.172947109498725
35	25.452248042860514	24.94794065121921	25.73326744016264	23.86654386575764
36	24.242584210348973	25.60488807520807	25.551424752619816	24.601102961823145
37	25.144160030550474	25.327575168757647	25.425067109947996	24.103197690743883
38	24.190019430997495	25.137645592083835	26.016196241842916	24.656138735075757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	221.0
1	187.0
2	153.0
3	153.0
4	471.5
5	790.0
6	790.0
7	871.0
8	952.0
9	946.0
10	940.0
11	940.0
12	1213.5
13	1487.0
14	1952.5
15	2418.0
16	2418.0
17	3690.0
18	4962.0
19	4962.0
20	5957.0
21	6952.0
22	7590.0
23	8228.0
24	8228.0
25	9570.0
26	10912.0
27	10912.0
28	14090.5
29	17269.0
30	20571.5
31	23874.0
32	23874.0
33	29782.0
34	35690.0
35	35690.0
36	39063.5
37	42437.0
38	47508.5
39	52580.0
40	52580.0
41	55323.0
42	58066.0
43	64421.5
44	70777.0
45	70777.0
46	73054.5
47	75332.0
48	75332.0
49	79112.0
50	82892.0
51	81710.5
52	80529.0
53	80529.0
54	75949.0
55	71369.0
56	71369.0
57	68701.0
58	66033.0
59	58760.5
60	51488.0
61	51488.0
62	48528.0
63	45568.0
64	37312.0
65	29056.0
66	29056.0
67	24212.0
68	19368.0
69	19368.0
70	15755.5
71	12143.0
72	9439.5
73	6736.0
74	6736.0
75	5084.5
76	3433.0
77	3433.0
78	3343.5
79	3254.0
80	2572.5
81	1891.0
82	1891.0
83	1764.0
84	1637.0
85	1637.0
86	1072.0
87	507.0
88	399.5
89	292.0
90	292.0
91	176.0
92	60.0
93	38.0
94	16.0
95	16.0
96	11.5
97	7.0
98	7.0
99	9.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00909775027237092
2	0.0
3	0.0
4	1.1231790459717183E-4
5	0.0
6	0.0
7	7.862253321802029E-4
8	0.0022463580919434368
9	0.0044927161838868735
10	0.0010108611413745467
11	0.0056158952298585915
12	2.2463580919434366E-4
13	1.1231790459717183E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	2.2463580919434366E-4
23	3.369537137915155E-4
24	0.0
25	0.0
26	0.0
27	1.1231790459717183E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	890330.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.80913420387149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.25925775980353	52.69800904998168
2	9.61941631238945	11.891355876307802
3	2.264602266355573	4.199193161986893
4	0.9233732115022817	2.2829159500001737
5	0.47502121430663213	1.468032499238231
6	0.27912182161737364	1.0351366878946395
7	0.19230177693834216	0.8320204436897464
8	0.14175452661096416	0.7009379647442684
9	0.09955688989312596	0.5538172651491852
>10	0.5761268627542915	6.919383791554829
>50	0.07887931170163892	3.445029644433824
>100	0.0839998246203445	10.429950451148306
>500	0.005673190741652019	2.608986373405768
>1k	9.150307647825838E-4	0.9352308404647254
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2866	0.3219031145754945	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2127	0.23890018307818447	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1138	0.12781777543158154	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1097	0.1232127413430975	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1040	0.11681062078105871	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	962	0.1080498242224793	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	934	0.10490492289375848	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	932	0.10468028708456416	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	931	0.10456796917996697	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	905	0.1016477036604405	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.10108611413745465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4
11	0.0	1.1231790459717183E-4	0.0	0.0	1.1231790459717183E-4
12	0.0	1.1231790459717183E-4	0.0	0.0	3.369537137915155E-4
13	0.0	1.1231790459717183E-4	0.0	0.0	5.615895229858592E-4
14	0.0	1.1231790459717183E-4	0.0	0.0	5.615895229858592E-4
15	0.0	1.1231790459717183E-4	0.0	0.0	5.615895229858592E-4
16	0.0	1.1231790459717183E-4	0.0	1.1231790459717183E-4	6.73907427583031E-4
17	0.0	1.1231790459717183E-4	0.0	1.1231790459717183E-4	6.73907427583031E-4
18	0.0	1.1231790459717183E-4	0.0	2.2463580919434366E-4	0.0010108611413745465
19	0.0	1.1231790459717183E-4	0.0	2.2463580919434366E-4	0.0010108611413745465
20	0.0	1.1231790459717183E-4	0.0	3.369537137915155E-4	0.0010108611413745465
21	0.0	1.1231790459717183E-4	0.0	5.615895229858592E-4	0.0011231790459717184
22	0.0	1.1231790459717183E-4	0.0	0.0011231790459717184	0.0011231790459717184
23	0.0	1.1231790459717183E-4	0.0	0.0015724506643604056	0.0011231790459717184
24	0.0	1.1231790459717183E-4	0.0	0.002920265519526468	0.0011231790459717184
25	0.0	1.1231790459717183E-4	0.0	0.0035941729471094985	0.0011231790459717184
26	0.0	1.1231790459717183E-4	0.0	0.004717351993081217	0.0011231790459717184
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	35	0.0020631002	22.863003	5
TACCGTC	175	0.0	20.119442	7
CGTGAGA	40	0.004484795	19.998386	12
ATACCGT	180	0.0	19.560568	6
ACCGTCG	165	0.0	19.39891	8
CCGTCGT	175	0.0	18.289373	9
CGTCGTA	185	0.0	18.163755	10
GCGACGC	45	0.008852868	17.776342	32
TCCTATA	65	2.0992261E-4	17.231314	2
ATCTAGG	75	3.2330507E-5	17.066246	1
TCTAGAT	180	0.0	16.889421	2
GTGTAGG	100	1.3138924E-6	15.999606	1
AGGGGCG	210	0.0	15.998708	32
GCTCGTT	60	0.002442641	15.998708	12
TTTCGTT	265	0.0	15.696846	28
GTCGTAG	205	0.0	15.6102495	11
GTCCTAC	135	7.903509E-9	15.407028	1
TAATACT	125	5.119182E-8	15.363074	4
TCGTTCT	265	0.0	15.093121	30
TTCGTTC	265	0.0	15.093121	29
>>END_MODULE