FastQCFastQC Report
Thu 2 Feb 2017
SRR4062837_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062837_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122329
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26320.23451233996448456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18570.1654595042986504No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15400.13721466700049628No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15390.1371255665673791No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15390.1371255665673791No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15100.13454165400698012No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14770.13160133971411236No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14650.1305321345167059No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14460.128839226287479No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14360.12794822195630692No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13600.12117658903939932No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13070.11645426608418745No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12740.11351395179131965No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12110.1079006245049357No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11560.10300010068348942No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11440.10193089548608296No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT11360.10121809202114532No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11360.10121809202114532No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11270.10041618812309046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3200.020.50002928
CTAGCGG3200.020.50002929
TAGCGGC3450.019.0145230
CGAGCCG3650.017.97182715
CGACATA450.00885234917.7770114
GTTCAAA4200.017.5433791
ACACCGT652.1039041E-417.2269546
AGCGGCG3900.016.82053631
AGGCCCG4150.016.19207210
GTAATAC600.00242373216.0178681
ATCGTTT3900.016.00002129
TACACCG600.002445467515.9964585
AACCGCG2150.015.6265367
GTACCGG1357.792551E-915.4246141
CGTTTAT3850.015.37664531
CGTGATT1255.1359166E-815.36002128
CGGCATC3650.015.34180425
CTAGCAC1153.3512515E-715.3016423
CGCATCG3700.015.13448113
CTATTCC4700.014.9747414
Produced by FastQC (version 0.11.5)