##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062837_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1122329
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.207683308548564	32.0	32.0	32.0	32.0	32.0
2	30.820891200352126	32.0	32.0	32.0	32.0	32.0
3	30.873893483996227	32.0	32.0	32.0	32.0	32.0
4	30.94931610962561	32.0	32.0	32.0	32.0	32.0
5	30.822756072417267	32.0	32.0	32.0	32.0	32.0
6	34.443688080767764	36.0	36.0	36.0	32.0	36.0
7	34.38532818808032	36.0	36.0	36.0	32.0	36.0
8	34.37084045765546	36.0	36.0	36.0	32.0	36.0
9	34.53417046160261	36.0	36.0	36.0	32.0	36.0
10	34.227261346717405	36.0	36.0	36.0	32.0	36.0
11	34.51045638132847	36.0	36.0	36.0	32.0	36.0
12	34.32091392096257	36.0	36.0	36.0	32.0	36.0
13	34.42893661306088	36.0	36.0	36.0	32.0	36.0
14	34.3238275051255	36.0	36.0	36.0	32.0	36.0
15	34.258461645382056	36.0	36.0	36.0	32.0	36.0
16	34.27954726287925	36.0	36.0	36.0	32.0	36.0
17	34.1967711785047	36.0	36.0	36.0	32.0	36.0
18	34.23562520437412	36.0	36.0	36.0	32.0	36.0
19	34.21054877847761	36.0	36.0	36.0	32.0	36.0
20	34.20655084204364	36.0	36.0	36.0	32.0	36.0
21	34.16674255053554	36.0	36.0	36.0	32.0	36.0
22	34.140748390177926	36.0	36.0	36.0	32.0	36.0
23	34.097575666315315	36.0	36.0	36.0	32.0	36.0
24	34.07709860477632	36.0	36.0	36.0	32.0	36.0
25	34.06112735214006	36.0	36.0	36.0	32.0	36.0
26	34.00138105671331	36.0	36.0	36.0	32.0	36.0
27	34.00950434320061	36.0	36.0	36.0	32.0	36.0
28	33.98393162789164	36.0	36.0	36.0	32.0	36.0
29	33.95822882595032	36.0	36.0	36.0	32.0	36.0
30	33.92791418559086	36.0	36.0	36.0	32.0	36.0
31	33.93916846129789	36.0	36.0	36.0	32.0	36.0
32	33.888981751340296	36.0	36.0	36.0	32.0	36.0
33	33.85435554102229	36.0	36.0	36.0	32.0	36.0
34	33.860626429505075	36.0	36.0	36.0	32.0	36.0
35	33.82002068912057	36.0	36.0	36.0	32.0	36.0
36	33.78093767513804	36.0	36.0	36.0	32.0	36.0
37	33.7736136195358	36.0	36.0	36.0	32.0	36.0
38	33.09570188420686	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	4.0
9	3.0
10	9.0
11	6.0
12	4.0
13	6.0
14	154.0
15	366.0
16	481.0
17	560.0
18	755.0
19	946.0
20	1294.0
21	1721.0
22	2306.0
23	3366.0
24	4703.0
25	6753.0
26	10236.0
27	14320.0
28	20602.0
29	28609.0
30	40118.0
31	56367.0
32	79394.0
33	124578.0
34	271284.0
35	453382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.196558749959856	17.530428514845834	11.641159405809876	25.631853329384434
2	16.816406946475055	19.736832721178192	37.18227365117855	26.264486681168208
3	18.82698584837146	23.80211066981642	28.620757563471138	28.75014591834098
4	12.597834454835766	15.228137830748176	35.93251026459854	36.241517449817515
5	14.773501840372727	36.27632588363284	33.572212020204454	15.37796025578998
6	34.387752556302814	35.426311831521026	16.786150879781598	13.399784732394568
7	30.3202807022546	30.251316905574843	21.112277337819247	18.31612505435131
8	28.333682311273474	32.56106742699308	19.509054863787227	19.59619539794623
9	27.41600925221371	14.138405418746425	18.79890513095126	39.64668019808861
10	16.175824606641758	26.244494677557174	31.23637312966053	26.34330758614053
11	37.8578664915518	20.965757263361045	22.12711093449142	19.049265310595725
12	24.929498025944728	23.628073908344316	28.45838356459763	22.984044501113328
13	29.43551549111025	19.167308268103852	25.36678215492536	26.030394085860532
14	23.874134772349784	19.8272574332946	24.76823304652804	31.530374747827572
15	25.20955981299583	27.10901031446003	22.262533040314977	25.418896832229166
16	25.878784422472357	25.512015340856436	23.753109873894168	24.856090362777035
17	24.122999708594847	25.68446792716475	25.271599404357175	24.920932959883224
18	25.346349739279823	24.264975817000455	26.267691726455883	24.120982717263846
19	25.7007567825736	24.936578359030197	25.30788566907047	24.05477918932574
20	25.975210956365224	23.922407263849163	24.930988086645815	25.171393693139798
21	27.245890479313395	24.05325981580223	24.26630977160617	24.434539933278206
22	26.022691731587805	24.03224211166941	24.8839171706353	25.061148986107483
23	24.229066253921832	23.864439341568627	25.64560438336439	26.260890021145155
24	24.890682602159526	24.966338172378855	25.148927410752712	24.994051814708904
25	25.069463380901713	24.26147816648755	25.199835319656312	25.469223132954426
26	24.642028381080706	25.27598658297064	25.749093708896087	24.332891327052568
27	25.641434617955	24.473602138560928	24.700289596792157	25.184673646691913
28	24.458067505435363	24.505114588159817	25.783494315144175	25.253323591260646
29	24.575334043226242	24.701867255966885	25.72081605011428	25.00198265069259
30	24.443398975143076	24.984607543595626	25.933370934587185	24.638622546674117
31	25.13265441597752	24.82159039167692	24.548395806026498	25.49735938631906
32	24.83241569995126	24.736360469084563	24.419502954277533	26.011720876686645
33	24.264538808024913	24.385990706212045	25.337289653421013	26.012180832342025
34	25.37096509737945	24.53154851863469	25.424342011463114	24.67314437252274
35	26.034360304660538	24.299591771991157	25.438816147685685	24.227231775662624
36	24.53947274619673	25.2318676304946	24.985296633731128	25.243362989577538
37	25.70350621437915	24.932278059160463	24.73392405875413	24.63029166770626
38	24.651910859240648	24.711254867367032	25.288834237750272	25.34800003564205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94.0
1	140.0
2	186.0
3	186.0
4	427.0
5	668.0
6	668.0
7	773.5
8	879.0
9	861.0
10	843.0
11	843.0
12	1110.5
13	1378.0
14	1935.0
15	2492.0
16	2492.0
17	3620.0
18	4748.0
19	4748.0
20	5697.0
21	6646.0
22	7295.5
23	7945.0
24	7945.0
25	9495.0
26	11045.0
27	11045.0
28	13966.5
29	16888.0
30	20890.0
31	24892.0
32	24892.0
33	31783.5
34	38675.0
35	38675.0
36	42905.0
37	47135.0
38	54074.5
39	61014.0
40	61014.0
41	64559.5
42	68105.0
43	77513.0
44	86921.0
45	86921.0
46	90058.5
47	93196.0
48	93196.0
49	98862.5
50	104529.0
51	105046.5
52	105564.0
53	105564.0
54	100912.0
55	96260.0
56	96260.0
57	93873.0
58	91486.0
59	82125.5
60	72765.0
61	72765.0
62	68850.5
63	64936.0
64	53232.0
65	41528.0
66	41528.0
67	34739.0
68	27950.0
69	27950.0
70	22367.5
71	16785.0
72	12984.5
73	9184.0
74	9184.0
75	6940.0
76	4696.0
77	4696.0
78	4623.5
79	4551.0
80	3625.5
81	2700.0
82	2700.0
83	2776.0
84	2852.0
85	2852.0
86	1874.5
87	897.0
88	775.5
89	654.0
90	654.0
91	487.5
92	321.0
93	261.0
94	201.0
95	201.0
96	193.0
97	185.0
98	185.0
99	360.0
100	535.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11948368081017241
2	0.05426216376837807
3	0.011226654572767879
4	0.002227510827930135
5	1.7820086623441077E-4
6	4.4550216558602695E-4
7	8.910043311720539E-5
8	3.5640173246882155E-4
9	2.6730129935161615E-4
10	5.346025987032323E-4
11	0.0016038077961096967
12	0.0017820086623441078
13	0.012206759337057137
14	0.007217135082493636
15	0.019156593120199156
16	0.008642742012368922
17	0.01603807796109697
18	0.0047223229552118854
19	0.006949833783142021
20	0.005256925553915118
21	0.005078724687680707
22	0.006237030318204376
23	0.0076626372480796635
24	0.012295859770174344
25	0.013810567133166834
26	0.015414374929276532
27	0.007038934216259225
28	0.004365921222743064
29	0.008375440713017306
30	0.00231661126104734
31	0.00463322252209468
32	0.0051678251207979125
33	0.006593432050673198
34	0.010781152407181851
35	0.014434270164987271
36	0.012206759337057137
37	0.008286340279900101
38	0.005256925553915118
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1122329.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.489441621199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.79912730278195	46.77276468056726
2	10.702816210819007	12.09192223046965
3	2.8973690531030774	4.910122799410048
4	1.2059909659036523	2.7250302505643105
5	0.6143490215201114	1.7352116593100535
6	0.37611580060203864	1.274794293655163
7	0.2529841254737477	1.0003652390927549
8	0.1724572547218985	0.7793611218211945
9	0.13090250127936515	0.6655148283680663
>10	0.665220525980679	7.009274436410156
>50	0.08026643662754747	3.19173457473409
>100	0.08783994675784668	10.689820350856923
>500	0.010287560194436215	3.956748742951219
>1k	0.0042732942346119665	3.19733479178917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2632	0.23451233996448456	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1857	0.1654595042986504	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1540	0.13721466700049628	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1539	0.1371255665673791	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1539	0.1371255665673791	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1510	0.13454165400698012	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1477	0.13160133971411236	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1465	0.1305321345167059	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1446	0.128839226287479	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1436	0.12794822195630692	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1360	0.12117658903939932	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1307	0.11645426608418745	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1274	0.11351395179131965	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1211	0.1079006245049357	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1156	0.10300010068348942	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1144	0.10193089548608296	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1136	0.10121809202114532	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1136	0.10121809202114532	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1127	0.10041618812309046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.5640173246882155E-4
2	0.0	0.0	0.0	0.0	3.5640173246882155E-4
3	0.0	0.0	0.0	0.0	3.5640173246882155E-4
4	0.0	0.0	0.0	0.0	3.5640173246882155E-4
5	0.0	0.0	0.0	0.0	3.5640173246882155E-4
6	0.0	0.0	0.0	0.0	3.5640173246882155E-4
7	0.0	0.0	0.0	0.0	3.5640173246882155E-4
8	0.0	0.0	0.0	0.0	3.5640173246882155E-4
9	0.0	0.0	0.0	0.0	3.5640173246882155E-4
10	0.0	0.0	0.0	0.0	4.455021655860269E-4
11	0.0	0.0	0.0	0.0	4.455021655860269E-4
12	0.0	0.0	0.0	0.0	8.019038980548484E-4
13	0.0	0.0	0.0	0.0	9.801047642892591E-4
14	0.0	0.0	0.0	0.0	9.801047642892591E-4
15	0.0	0.0	0.0	0.0	9.801047642892591E-4
16	0.0	0.0	0.0	0.0	9.801047642892591E-4
17	0.0	0.0	0.0	0.0	9.801047642892591E-4
18	0.0	0.0	0.0	0.0	0.0010692051974064646
19	0.0	0.0	0.0	0.0	0.0010692051974064646
20	0.0	0.0	0.0	8.910043311720539E-5	0.0010692051974064646
21	0.0	0.0	0.0	8.910043311720539E-5	0.0010692051974064646
22	0.0	0.0	0.0	1.7820086623441077E-4	0.0010692051974064646
23	0.0	0.0	0.0	3.5640173246882155E-4	0.0010692051974064646
24	0.0	0.0	0.0	5.346025987032323E-4	0.0010692051974064646
25	0.0	0.0	0.0	7.128034649376431E-4	0.0010692051974064646
26	0.0	0.0	0.0	0.0010692051974064646	0.00115830563052367
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	320	0.0	20.500029	28
CTAGCGG	320	0.0	20.500029	29
TAGCGGC	345	0.0	19.01452	30
CGAGCCG	365	0.0	17.971827	15
CGACATA	45	0.008852349	17.77701	14
GTTCAAA	420	0.0	17.543379	1
ACACCGT	65	2.1039041E-4	17.226954	6
AGCGGCG	390	0.0	16.820536	31
AGGCCCG	415	0.0	16.192072	10
GTAATAC	60	0.002423732	16.017868	1
ATCGTTT	390	0.0	16.000021	29
TACACCG	60	0.0024454675	15.996458	5
AACCGCG	215	0.0	15.626536	7
GTACCGG	135	7.792551E-9	15.424614	1
CGTTTAT	385	0.0	15.376645	31
CGTGATT	125	5.1359166E-8	15.360021	28
CGGCATC	365	0.0	15.341804	25
CTAGCAC	115	3.3512515E-7	15.301642	3
CGCATCG	370	0.0	15.134481	13
CTATTCC	470	0.0	14.974741	4
>>END_MODULE