FastQCFastQC Report
Thu 2 Feb 2017
SRR4062837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122329
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27620.2460953962697213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21290.18969482210653027No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17280.1539655484265309No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15870.14140238735700494No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15570.13872937436348878No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15540.13846207306413716No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15340.13668006440179306No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15070.13427435270762852No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14450.1287501258543618No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13910.1239387024660327No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13820.12313679856797784No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13730.12233489466992298No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13370.1191272790777036No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12870.11467225742184332No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12440.1108409387978035No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11860.10567311367700558No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG11640.10371290414842706No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11580.10317830154972384No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11570.10308920111660663No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11380.10139629288737972No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11350.10112899158802811No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC601.1367307E-418.6697084
TAGGACA1006.8010195E-817.6028674
GCTCGTA4400.016.36265826
TACGACG4150.016.195415
ACGACGG4250.015.8143416
CGACGGT4150.015.8098047
CTAGCGG3400.015.52848329
GTTTTCG3950.015.39148328
GTATAGT1153.3434299E-715.3047941
CTACGAC4400.015.2752174
AAGCTCG4400.015.27181324
CTATACT951.4301249E-515.1603654
TCTAGCG3500.015.0848128
CTCGTAG4700.014.97782627
GACGGTA4400.014.9108578
ACGGTAT4400.014.91019259
TAATACT2050.014.8316854
AATTAGA2400.014.6670952
CAAGACG4150.014.6529894
TCTTATA1103.5409612E-614.5458812
Produced by FastQC (version 0.11.5)