FastQCFastQC Report
Thu 2 Feb 2017
SRR4062836_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062836_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687988
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36530.2164114910769508No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24600.14573563319170516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24330.14413609575423522No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24250.1436621587357256No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22740.13471659751135673No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22090.13086585923596614No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21100.1250008886319097No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21050.12470467799534118No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20990.124349225231459No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20650.12233499290279315No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20440.12109090822920543No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19490.1154629061344038No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19280.11421882146081608No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18490.10953869340303367No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17700.10485856534525127No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17520.10379220705360466No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA17430.10325902790778133No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17330.10266660663464432No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17080.10118555345180179No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17030.10088934281523329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA453.5378285E-421.33323532
GAATCGT450.00886276217.774016
TATACTG1550.017.5436275
ATCGTTT3750.017.06658729
TACCGTC4700.016.6797857
TCTAGCG4400.016.3625928
TAACGAA2900.015.99992713
ACCGTCG4850.015.8340418
ATACCGT5100.015.6829496
CGTCGTA5100.015.37247810
GCATCGT4300.015.25438827
TAGGACG1052.1918058E-615.2344134
AATAGCG3800.015.1537855
GGCATCG4400.014.90858126
CGTTATT3900.014.7748532
TAGCGGC4900.014.69380930
CTAGCGG4900.014.69380929
AAGACGG6100.014.6845415
GTATTAC1205.364782E-714.6800831
CGATAAC2950.014.64410
Produced by FastQC (version 0.11.5)