##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062836_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1687988
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268355580726876	32.0	32.0	32.0	32.0	32.0
2	30.914971551930464	32.0	32.0	32.0	32.0	32.0
3	30.958869375848643	32.0	32.0	32.0	32.0	32.0
4	31.024863328412287	32.0	32.0	32.0	32.0	32.0
5	30.959575542006224	32.0	32.0	32.0	32.0	32.0
6	34.61391372450515	36.0	36.0	36.0	32.0	36.0
7	34.52859972938196	36.0	36.0	36.0	32.0	36.0
8	34.52574129673908	36.0	36.0	36.0	32.0	36.0
9	34.63991746386823	36.0	36.0	36.0	32.0	36.0
10	34.391742713810764	36.0	36.0	36.0	32.0	36.0
11	34.62584508894613	36.0	36.0	36.0	32.0	36.0
12	34.4571104770887	36.0	36.0	36.0	32.0	36.0
13	34.53222179304592	36.0	36.0	36.0	32.0	36.0
14	34.44208371149558	36.0	36.0	36.0	32.0	36.0
15	34.394040123507985	36.0	36.0	36.0	32.0	36.0
16	34.40812967864701	36.0	36.0	36.0	32.0	36.0
17	34.33980395595229	36.0	36.0	36.0	32.0	36.0
18	34.35782007928967	36.0	36.0	36.0	32.0	36.0
19	34.342273760240005	36.0	36.0	36.0	32.0	36.0
20	34.32234944798186	36.0	36.0	36.0	32.0	36.0
21	34.31144948897741	36.0	36.0	36.0	32.0	36.0
22	34.296397249269546	36.0	36.0	36.0	32.0	36.0
23	34.2358660132655	36.0	36.0	36.0	32.0	36.0
24	34.21430958039986	36.0	36.0	36.0	32.0	36.0
25	34.209041178017856	36.0	36.0	36.0	32.0	36.0
26	34.15980267632234	36.0	36.0	36.0	32.0	36.0
27	34.16416645142027	36.0	36.0	36.0	32.0	36.0
28	34.14649689452769	36.0	36.0	36.0	32.0	36.0
29	34.11316845854354	36.0	36.0	36.0	32.0	36.0
30	34.08637620646592	36.0	36.0	36.0	32.0	36.0
31	34.102740066872514	36.0	36.0	36.0	32.0	36.0
32	34.057444721171	36.0	36.0	36.0	32.0	36.0
33	34.02240359528622	36.0	36.0	36.0	32.0	36.0
34	34.02676796280542	36.0	36.0	36.0	32.0	36.0
35	33.98691874586786	36.0	36.0	36.0	32.0	36.0
36	33.9547638964258	36.0	36.0	36.0	32.0	36.0
37	33.96014900579862	36.0	36.0	36.0	32.0	36.0
38	33.37490432396439	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	5.0
10	9.0
11	15.0
12	9.0
13	11.0
14	215.0
15	456.0
16	581.0
17	658.0
18	799.0
19	1029.0
20	1410.0
21	1978.0
22	3048.0
23	4658.0
24	7014.0
25	10653.0
26	15637.0
27	22533.0
28	31580.0
29	42438.0
30	56891.0
31	76575.0
32	104099.0
33	155866.0
34	354204.0
35	795609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.541705023203725	17.045368270207174	11.321070313463856	26.091856393125244
2	16.973667753593766	19.666524206307102	37.02833222388129	26.331475816217836
3	18.633056441005344	23.991989477301544	28.452582682577116	28.922371399116
4	12.663913011022917	15.293808184897786	35.84942100953945	36.19285779453985
5	14.979229660944654	36.164975497398075	33.317614384970476	15.538180456686792
6	34.28873395051964	35.42020269161478	16.80751524156345	13.483548116302119
7	30.384043014523797	30.270298130081493	21.128882432813505	18.216776422581205
8	28.49224695524004	32.18411096800795	19.608028995569857	19.715613081182145
9	27.868224103621653	14.029906628427828	18.35517690598328	39.74669236196725
10	16.252128280988778	26.018109197846893	31.04043763499848	26.68932488616585
11	37.87942339729234	20.91571107303745	22.130730895829036	19.074134633841165
12	25.30790362493609	23.519221871302832	28.112849514824813	23.060024988936263
13	29.600616678279746	18.979861970242073	25.061975930112958	26.357545421365224
14	24.15034242253501	19.55087814094691	24.647312787388756	31.651466649129322
15	25.558388673952447	26.577185423615724	22.154103600483296	25.71032230194853
16	26.274309054939664	25.309975074016606	23.268438088229804	25.147277782813926
17	24.56885083972243	25.443799955085233	24.80373491755652	25.18361428763582
18	25.69054538021986	24.108051104771892	25.718686774433397	24.482716740574855
19	25.99107276161406	24.65560103041233	25.015226309166355	24.338099898807254
20	26.1246417871275	23.722119826316003	24.50196783800869	25.651270548547807
21	27.660572825421724	23.881273479911773	23.880147806803688	24.578005887862815
22	26.43997886095628	23.67466253365184	24.48349163917643	25.401866966215447
23	24.58414009010397	23.67290979886576	25.22899252767462	26.51395758335565
24	25.175425135253477	24.737447393197005	24.736203138247983	25.350924333301535
25	25.299756355379856	24.02234041040372	24.785744419637567	25.89215881457886
26	25.00404407298885	25.042736815285117	25.258479961840543	24.694739149885493
27	25.8809024299061	24.28608585040196	24.300364429630005	25.532647290061934
28	24.91265737816319	24.188677836806004	25.378267461266795	25.520397323764005
29	24.877785197004158	24.503394049201123	25.277712439870196	25.341108313924522
30	24.75434582467881	24.69409493928109	25.502061089776785	25.04949814626332
31	25.55721771792677	24.549157382420113	24.010142811016088	25.883482088637027
32	25.155743352728553	24.471390916259693	24.047366122925126	26.325499608086627
33	24.724738246571967	24.00737507168892	24.96291135220707	26.30497532953204
34	25.690421638454936	24.25101005881715	24.991867982246426	25.066700320481488
35	26.454171841374276	23.923621315687186	24.96118969293048	24.661017150008057
36	24.90038459692571	24.926751201754296	24.493035365023424	25.679828836296565
37	26.114689683284514	24.727044999952604	24.36130781408488	24.796957502678005
38	25.020306430102927	24.33347216552747	24.98327792882115	25.66294347554845
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	571.0
1	434.0
2	297.0
3	297.0
4	649.5
5	1002.0
6	1002.0
7	1121.5
8	1241.0
9	1200.5
10	1160.0
11	1160.0
12	1523.5
13	1887.0
14	2437.0
15	2987.0
16	2987.0
17	4562.0
18	6137.0
19	6137.0
20	7459.0
21	8781.0
22	9576.0
23	10371.0
24	10371.0
25	12256.0
26	14141.0
27	14141.0
28	18033.0
29	21925.0
30	27399.5
31	32874.0
32	32874.0
33	43312.0
34	53750.0
35	53750.0
36	60017.0
37	66284.0
38	76674.5
39	87065.0
40	87065.0
41	94059.0
42	101053.0
43	116341.0
44	131629.0
45	131629.0
46	135232.0
47	138835.0
48	138835.0
49	148927.0
50	159019.0
51	160947.0
52	162875.0
53	162875.0
54	155239.0
55	147603.0
56	147603.0
57	144521.0
58	141439.0
59	126701.5
60	111964.0
61	111964.0
62	106722.0
63	101480.0
64	83682.0
65	65884.0
66	65884.0
67	55288.5
68	44693.0
69	44693.0
70	36027.0
71	27361.0
72	21307.5
73	15254.0
74	15254.0
75	11579.0
76	7904.0
77	7904.0
78	7661.0
79	7418.0
80	5856.5
81	4295.0
82	4295.0
83	4400.5
84	4506.0
85	4506.0
86	2973.5
87	1441.0
88	1239.0
89	1037.0
90	1037.0
91	742.0
92	447.0
93	406.5
94	366.0
95	366.0
96	310.0
97	254.0
98	254.0
99	506.0
100	758.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12156484524771503
2	0.05740562136697654
3	0.012322362481249866
4	0.002962106365685064
5	2.3696850925480513E-4
6	2.9621063656850644E-4
7	0.0
8	3.554527638822077E-4
9	5.924212731370128E-5
10	3.554527638822077E-4
11	0.001895748074038441
12	0.0024289272198617525
13	0.013981142046033502
14	0.009537982497505907
15	0.024230030071303824
16	0.011315246316916945
17	0.020557018177854346
18	0.004917096567037206
19	0.007109055277644154
20	0.005628002094801622
21	0.006457391877193439
22	0.006872086768389349
23	0.009478740370192206
24	0.013684931409464996
25	0.016824764157091162
26	0.019727628395462528
27	0.008590108460486686
28	0.005628002094801622
29	0.010722825043779933
30	0.002725137856430259
31	0.0055095178401742195
32	0.006812844641075648
33	0.00829389782391818
34	0.012737057372445775
35	0.017298701175600777
36	0.014632805446484215
37	0.00971570887944701
38	0.005864970604056427
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1687988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.04142270638277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95564233042086	43.20916664938624
2	12.338063347704056	13.335329935028058
3	3.5921656794420995	5.823772317422736
4	1.467593752595575	3.17243417381056
5	0.7026045047966907	1.898487351956335
6	0.45339145475177656	1.4701151554621543
7	0.2811903361743698	1.0637148072704292
8	0.19991254948729625	0.8642846872922912
9	0.14409279733635455	0.7008281792819172
>10	0.7058898602708386	6.739377369929729
>50	0.06384281247822317	2.413793399465755
>100	0.07659929441122432	9.291996999295073
>500	0.013484745209038036	5.1964168288349475
>1k	0.0055265349217369	4.820282145563798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3653	0.2164114910769508	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2460	0.14573563319170516	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2433	0.14413609575423522	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2425	0.1436621587357256	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2274	0.13471659751135673	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2209	0.13086585923596614	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2110	0.1250008886319097	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2105	0.12470467799534118	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2099	0.124349225231459	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2065	0.12233499290279315	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2044	0.12109090822920543	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1949	0.1154629061344038	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1928	0.11421882146081608	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1849	0.10953869340303367	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1770	0.10485856534525127	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1752	0.10379220705360466	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1743	0.10325902790778133	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1733	0.10266660663464432	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1708	0.10118555345180179	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1703	0.10088934281523329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.924212731370128E-5
7	0.0	0.0	0.0	0.0	5.924212731370128E-5
8	0.0	0.0	0.0	5.924212731370128E-5	5.924212731370128E-5
9	0.0	0.0	0.0	5.924212731370128E-5	5.924212731370128E-5
10	0.0	0.0	0.0	5.924212731370128E-5	5.924212731370128E-5
11	0.0	0.0	0.0	5.924212731370128E-5	5.924212731370128E-5
12	0.0	0.0	0.0	5.924212731370128E-5	1.1848425462740256E-4
13	0.0	0.0	0.0	5.924212731370128E-5	1.1848425462740256E-4
14	0.0	0.0	0.0	5.924212731370128E-5	1.1848425462740256E-4
15	0.0	0.0	0.0	5.924212731370128E-5	1.1848425462740256E-4
16	5.924212731370128E-5	0.0	0.0	1.1848425462740256E-4	1.1848425462740256E-4
17	5.924212731370128E-5	0.0	0.0	1.1848425462740256E-4	1.1848425462740256E-4
18	5.924212731370128E-5	0.0	0.0	1.1848425462740256E-4	1.1848425462740256E-4
19	5.924212731370128E-5	0.0	0.0	1.1848425462740256E-4	1.1848425462740256E-4
20	5.924212731370128E-5	0.0	0.0	1.7772638194110384E-4	1.1848425462740256E-4
21	5.924212731370128E-5	0.0	0.0	3.554527638822077E-4	1.1848425462740256E-4
22	5.924212731370128E-5	0.0	0.0	4.7393701850961025E-4	1.1848425462740256E-4
23	5.924212731370128E-5	0.0	5.924212731370128E-5	7.701476550781166E-4	1.1848425462740256E-4
24	5.924212731370128E-5	0.0	5.924212731370128E-5	0.001481053182842532	1.1848425462740256E-4
25	5.924212731370128E-5	0.0	5.924212731370128E-5	0.0018365059467247398	1.1848425462740256E-4
26	5.924212731370128E-5	0.0	5.924212731370128E-5	0.002488169347175454	1.1848425462740256E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	45	3.5378285E-4	21.333235	32
GAATCGT	45	0.008862762	17.77401	6
TATACTG	155	0.0	17.543627	5
ATCGTTT	375	0.0	17.066587	29
TACCGTC	470	0.0	16.679785	7
TCTAGCG	440	0.0	16.36259	28
TAACGAA	290	0.0	15.999927	13
ACCGTCG	485	0.0	15.834041	8
ATACCGT	510	0.0	15.682949	6
CGTCGTA	510	0.0	15.372478	10
GCATCGT	430	0.0	15.254388	27
TAGGACG	105	2.1918058E-6	15.234413	4
AATAGCG	380	0.0	15.153785	5
GGCATCG	440	0.0	14.908581	26
CGTTATT	390	0.0	14.774853	2
TAGCGGC	490	0.0	14.693809	30
CTAGCGG	490	0.0	14.693809	29
AAGACGG	610	0.0	14.684541	5
GTATTAC	120	5.364782E-7	14.680083	1
CGATAAC	295	0.0	14.644	10
>>END_MODULE