FastQCFastQC Report
Thu 2 Feb 2017
SRR4062836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687988
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42810.2536155470299552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30800.18246575212619995No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26340.15604376334428918No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24520.14526169617319554No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23910.14164792640705975No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22490.13323554432851417No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22010.13039192221745652No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21790.1290885954165551No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21430.12695587883326184No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21420.12689663670594814No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21070.12482316224996859No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20830.12340135119443976No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20470.12126863461114651No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20080.11895819164591218No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19580.1159960852802271No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18030.1068135555466034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17010.10077085856060589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG308.4140454E-426.6690865
AACGTAG450.00884732117.779398
ACCGTCG4900.017.6342538
AACCGCT652.0996816E-417.2323327
CGTCGTA5050.017.1094510
TACCGTC5150.017.088937
GTATTAC1102.0200787E-716.0005041
GTATATA1900.016.0005041
GCGACGC1102.0214429E-715.99955732
ATACCGT5550.015.8572936
CCGTCGT5550.015.8568249
GTCGTAG5600.015.42905811
CGTCTTA1357.91988E-915.4069815
GGTCTAT1052.1855867E-615.2385761
AGGGGCG4550.015.12045932
TACGCTA5200.015.0778459
GTATCAA33550.014.6889871
GTGTAGG2300.014.6091551
GTATTAG4550.014.41803551
ATACGCT5450.014.3866268
Produced by FastQC (version 0.11.5)