##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062836_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1687988
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238131432213972	32.0	32.0	32.0	32.0	32.0
2	31.323918179513125	32.0	32.0	32.0	32.0	32.0
3	31.39158216764574	32.0	32.0	32.0	32.0	32.0
4	31.49138500984604	32.0	32.0	32.0	32.0	32.0
5	31.420638653829293	32.0	32.0	32.0	32.0	32.0
6	34.94799963032913	36.0	36.0	36.0	36.0	36.0
7	34.97712898432927	36.0	36.0	36.0	36.0	36.0
8	34.91653317440645	36.0	36.0	36.0	36.0	36.0
9	35.013601400009954	36.0	36.0	36.0	36.0	36.0
10	34.86386040659057	36.0	36.0	36.0	32.0	36.0
11	35.03132723692349	36.0	36.0	36.0	36.0	36.0
12	34.93488223849933	36.0	36.0	36.0	32.0	36.0
13	34.9835834141001	36.0	36.0	36.0	36.0	36.0
14	34.93515889923388	36.0	36.0	36.0	32.0	36.0
15	34.90628724848755	36.0	36.0	36.0	32.0	36.0
16	34.913276634668016	36.0	36.0	36.0	32.0	36.0
17	34.8736205470655	36.0	36.0	36.0	32.0	36.0
18	34.87220584506525	36.0	36.0	36.0	32.0	36.0
19	34.86991969137221	36.0	36.0	36.0	32.0	36.0
20	34.85284077848895	36.0	36.0	36.0	32.0	36.0
21	34.84258655867222	36.0	36.0	36.0	32.0	36.0
22	34.82665161126738	36.0	36.0	36.0	32.0	36.0
23	34.76836861399489	36.0	36.0	36.0	32.0	36.0
24	34.74941291051832	36.0	36.0	36.0	32.0	36.0
25	34.73643473768771	36.0	36.0	36.0	32.0	36.0
26	34.664994656360115	36.0	36.0	36.0	32.0	36.0
27	34.653585807482045	36.0	36.0	36.0	32.0	36.0
28	34.6204493159904	36.0	36.0	36.0	32.0	36.0
29	34.589210942257886	36.0	36.0	36.0	32.0	36.0
30	34.56938378708853	36.0	36.0	36.0	32.0	36.0
31	34.56641812619521	36.0	36.0	36.0	32.0	36.0
32	34.5380885409138	36.0	36.0	36.0	32.0	36.0
33	34.502250608416645	36.0	36.0	36.0	32.0	36.0
34	34.49691881695841	36.0	36.0	36.0	32.0	36.0
35	34.465717173344835	36.0	36.0	36.0	32.0	36.0
36	34.43718024061783	36.0	36.0	36.0	32.0	36.0
37	34.429740021848495	36.0	36.0	36.0	32.0	36.0
38	34.00892008710962	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	29.0
22	123.0
23	377.0
24	1035.0
25	2699.0
26	5831.0
27	11074.0
28	19306.0
29	30682.0
30	46193.0
31	66655.0
32	95304.0
33	152293.0
34	374459.0
35	881917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94471039908996	18.263401744241158	11.989880557398806	26.802007299270077
2	15.956333812799617	20.718689943293437	36.255707978966676	27.06926826494027
3	17.676310495098306	24.736491017708655	28.768806413315733	28.818392073877302
4	12.146953738387795	16.148169387560447	35.88285928742342	35.822017586628334
5	14.438855275544181	36.66473734691085	32.99966172725264	15.89674565029233
6	34.28699391642234	35.84174522671087	16.452081680723847	13.419179176142945
7	30.19305870197822	30.481864757011916	21.04261651769211	18.282460023317757
8	28.312939662618742	32.448745276792685	19.381234663276345	19.85708039731223
9	27.779186478996067	13.828008640209678	18.199715389692177	40.19308949110208
10	16.03685112569395	26.312322235854552	31.054259496996707	26.59656714145479
11	37.971456518557886	20.866399941702053	22.004335613707365	19.1578079260327
12	25.099823221073187	23.613020028625865	28.086640631664757	23.200516118636198
13	29.610933253079995	18.993677680173082	25.031753780240145	26.363635286506774
14	24.04815674045076	19.559321511764303	24.659002315182335	31.733519432602602
15	25.544257423630974	26.695272715208873	22.041092709189876	25.719377151970274
16	26.23835373139722	25.383370843495833	23.171031530456098	25.207243894650848
17	24.502129162055656	25.515465749756515	24.650886143740358	25.331518944447474
18	25.672161176501255	24.07955506792702	25.71019462223665	24.53808913333507
19	25.935966369429163	24.62896655663429	25.028910158129086	24.406156915807458
20	26.205281080197253	23.598627478394395	24.505446721185223	25.690644720223126
21	27.56523310337473	23.90252283047539	23.873494136500028	24.658749929649847
22	26.310791308942953	23.74116403671116	24.55284042303618	25.395204231309705
23	24.522685049147384	23.509583029223023	25.232170447113244	26.735561474516345
24	25.175475181103184	24.677367374649585	24.775827790244954	25.371329654002277
25	25.228215620143995	23.956108666713664	24.896696479297532	25.91897923384481
26	25.086878682643103	24.885988390898977	25.246477162725284	24.780655763732636
27	25.967276979785375	24.142084116811397	24.328519312363966	25.562119591039263
28	24.89241629679832	24.08784896575094	25.430808749825236	25.588925987625505
29	24.960070806190565	24.29235278923784	25.258295675087737	25.48928072948386
30	24.755863193340236	24.560127204695767	25.614340860243086	25.069668741720914
31	25.597812306722563	24.281570722066746	24.170491733353554	25.95012523785714
32	25.22073616637085	24.28471055481437	24.272743645097	26.221809633717776
33	24.760128626506823	23.854257257753016	25.089040917352495	26.29657319838767
34	25.676900546686348	24.002718028801155	25.183354384035905	25.13702704047659
35	26.460318438282737	23.833937208084418	25.053851093728152	24.651893259904693
36	25.03661163467987	24.77191780984225	24.50384718374775	25.68762337173013
37	26.21428588354894	24.559297814913375	24.406867821335222	24.819548480202467
38	25.034079053926362	24.225186568379968	25.067313907038386	25.673420470655284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	301.0
1	244.5
2	188.0
3	188.0
4	538.5
5	889.0
6	889.0
7	1055.0
8	1221.0
9	1144.0
10	1067.0
11	1067.0
12	1478.0
13	1889.0
14	2437.5
15	2986.0
16	2986.0
17	4908.0
18	6830.0
19	6830.0
20	8003.0
21	9176.0
22	9879.5
23	10583.0
24	10583.0
25	12391.5
26	14200.0
27	14200.0
28	17962.5
29	21725.0
30	27084.0
31	32443.0
32	32443.0
33	43365.0
34	54287.0
35	54287.0
36	60284.5
37	66282.0
38	76705.5
39	87129.0
40	87129.0
41	94014.0
42	100899.0
43	116010.0
44	131121.0
45	131121.0
46	135000.0
47	138879.0
48	138879.0
49	149491.0
50	160103.0
51	161285.0
52	162467.0
53	162467.0
54	155152.5
55	147838.0
56	147838.0
57	145060.5
58	142283.0
59	127513.0
60	112743.0
61	112743.0
62	107696.5
63	102650.0
64	84131.5
65	65613.0
66	65613.0
67	55290.0
68	44967.0
69	44967.0
70	36188.5
71	27410.0
72	21380.0
73	15350.0
74	15350.0
75	11455.0
76	7560.0
77	7560.0
78	7441.5
79	7323.0
80	5692.0
81	4061.0
82	4061.0
83	3866.5
84	3672.0
85	3672.0
86	2320.0
87	968.0
88	825.5
89	683.0
90	683.0
91	409.0
92	135.0
93	82.0
94	29.0
95	29.0
96	23.0
97	17.0
98	17.0
99	19.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00876783484242779
2	0.0
3	0.0
4	5.924212731370128E-5
5	5.924212731370128E-5
6	5.924212731370128E-5
7	2.3696850925480513E-4
8	0.002725137856430259
9	0.003909980402704284
10	4.1469489119590896E-4
11	0.006338907622566037
12	2.3696850925480513E-4
13	0.0
14	0.0
15	0.0
16	5.924212731370128E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.7772638194110384E-4
22	0.0
23	2.3696850925480513E-4
24	0.0
25	5.924212731370128E-5
26	1.1848425462740256E-4
27	1.1848425462740256E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.924212731370128E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1687988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.108188160447575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.46418958462473	40.88626754732657
2	13.215205278803785	13.772408064936956
3	3.8526670542171995	6.0226649934212135
4	1.5788633090617499	3.2908682555286632
5	0.804165553536283	2.095180498790956
6	0.4837078920047394	1.5123085110765853
7	0.316507423156922	1.1544839852027506
8	0.21433184188828716	0.8934755156712134
9	0.1533524324395178	0.7191825663978647
>10	0.7488043325409957	6.903947469939192
>50	0.06713755055326308	2.4964803061694356
>100	0.08053322919140471	9.57091869816896
>500	0.013422003373162326	4.887763761226632
>1k	0.007112514608000548	5.7940498261430635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4281	0.2536155470299552	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3080	0.18246575212619995	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2634	0.15604376334428918	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2452	0.14526169617319554	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2391	0.14164792640705975	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2249	0.13323554432851417	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2201	0.13039192221745652	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2179	0.1290885954165551	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2143	0.12695587883326184	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2142	0.12689663670594814	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2107	0.12482316224996859	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2083	0.12340135119443976	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2047	0.12126863461114651	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2008	0.11895819164591218	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1958	0.1159960852802271	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1803	0.1068135555466034	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1701	0.10077085856060589	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.924212731370128E-5
2	0.0	0.0	0.0	0.0	5.924212731370128E-5
3	0.0	0.0	0.0	1.1848425462740256E-4	5.924212731370128E-5
4	0.0	0.0	0.0	1.1848425462740256E-4	5.924212731370128E-5
5	0.0	0.0	0.0	1.1848425462740256E-4	5.924212731370128E-5
6	0.0	0.0	5.924212731370128E-5	1.1848425462740256E-4	5.924212731370128E-5
7	0.0	0.0	5.924212731370128E-5	1.1848425462740256E-4	5.924212731370128E-5
8	0.0	0.0	5.924212731370128E-5	1.1848425462740256E-4	5.924212731370128E-5
9	0.0	0.0	5.924212731370128E-5	1.7772638194110384E-4	5.924212731370128E-5
10	0.0	0.0	5.924212731370128E-5	1.7772638194110384E-4	5.924212731370128E-5
11	0.0	0.0	1.1848425462740256E-4	1.7772638194110384E-4	5.924212731370128E-5
12	0.0	0.0	1.1848425462740256E-4	1.7772638194110384E-4	5.924212731370128E-5
13	0.0	0.0	1.1848425462740256E-4	1.7772638194110384E-4	1.1848425462740256E-4
14	0.0	0.0	1.1848425462740256E-4	1.7772638194110384E-4	1.1848425462740256E-4
15	0.0	0.0	1.1848425462740256E-4	1.7772638194110384E-4	1.7772638194110384E-4
16	0.0	0.0	1.1848425462740256E-4	2.3696850925480513E-4	1.7772638194110384E-4
17	0.0	0.0	1.1848425462740256E-4	2.3696850925480513E-4	1.7772638194110384E-4
18	0.0	0.0	1.1848425462740256E-4	2.3696850925480513E-4	1.7772638194110384E-4
19	0.0	0.0	1.1848425462740256E-4	2.3696850925480513E-4	1.7772638194110384E-4
20	0.0	0.0	1.1848425462740256E-4	2.962106365685064E-4	1.7772638194110384E-4
21	0.0	0.0	1.1848425462740256E-4	4.1469489119590896E-4	1.7772638194110384E-4
22	0.0	0.0	1.1848425462740256E-4	5.331791458233115E-4	1.7772638194110384E-4
23	0.0	0.0	1.1848425462740256E-4	8.886319097055192E-4	1.7772638194110384E-4
24	0.0	0.0	1.1848425462740256E-4	0.0015402953101562333	1.7772638194110384E-4
25	0.0	0.0	1.1848425462740256E-4	0.001954990201352142	1.7772638194110384E-4
26	0.0	0.0	1.1848425462740256E-4	0.002369685092548051	1.7772638194110384E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	30	8.4140454E-4	26.669086	5
AACGTAG	45	0.008847321	17.77939	8
ACCGTCG	490	0.0	17.634253	8
AACCGCT	65	2.0996816E-4	17.232332	7
CGTCGTA	505	0.0	17.10945	10
TACCGTC	515	0.0	17.08893	7
GTATTAC	110	2.0200787E-7	16.000504	1
GTATATA	190	0.0	16.000504	1
GCGACGC	110	2.0214429E-7	15.999557	32
ATACCGT	555	0.0	15.857293	6
CCGTCGT	555	0.0	15.856824	9
GTCGTAG	560	0.0	15.429058	11
CGTCTTA	135	7.91988E-9	15.40698	15
GGTCTAT	105	2.1855867E-6	15.238576	1
AGGGGCG	455	0.0	15.120459	32
TACGCTA	520	0.0	15.077845	9
GTATCAA	3355	0.0	14.688987	1
GTGTAGG	230	0.0	14.609155	1
GTATTAG	455	0.0	14.4180355	1
ATACGCT	545	0.0	14.386626	8
>>END_MODULE