FastQCFastQC Report
Thu 2 Feb 2017
SRR4062835_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062835_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823543
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51580.2828559567830317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34090.18694376825772685No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23680.12985709687131042No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22210.12179586661789715No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21890.12004104098450104No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20160.11055401490395345No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19130.1049056698964598No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18640.10221859314532204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18430.10106698882340587No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18390.10084763561923137No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18250.10007989940462056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG753.2388707E-517.0645185
TCTAGCG4000.016.39928428
GATATAC4200.016.018631
TAGCGGC4100.015.999330
CTAGCGG4100.015.999329
AACCGCG2500.015.9984237
ATAGCGT3650.015.7788346
GTATTAG4450.015.4786761
ATAAGGC1357.932613E-915.4054673
GTCCTAA3250.015.2793071
AATAGCG3700.015.1332285
GTATCAA37550.014.9308121
TAGCGTA3650.014.9026417
TAGGACG3950.014.5800414
CGTATAT3750.014.50643110
GTAGGAC7200.014.220433
AGCGTAT3850.014.1284778
ACCCGTT1707.6033757E-1014.11703130
CAAGACG5800.014.0668074
TTTTCGG4800.013.99938829
Produced by FastQC (version 0.11.5)