##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062835_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1823543
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.204352186924027	32.0	32.0	32.0	32.0	32.0
2	30.78874202582555	32.0	32.0	32.0	32.0	32.0
3	30.838110206340076	32.0	32.0	32.0	32.0	32.0
4	30.915324179358535	32.0	32.0	32.0	32.0	32.0
5	30.82480259582582	32.0	32.0	32.0	32.0	32.0
6	34.47608200080832	36.0	36.0	36.0	32.0	36.0
7	34.39506005616539	36.0	36.0	36.0	32.0	36.0
8	34.37387273017417	36.0	36.0	36.0	32.0	36.0
9	34.51780572215736	36.0	36.0	36.0	32.0	36.0
10	34.21538236279594	36.0	36.0	36.0	32.0	36.0
11	34.51653292519014	36.0	36.0	36.0	32.0	36.0
12	34.320919221537416	36.0	36.0	36.0	32.0	36.0
13	34.42170543825948	36.0	36.0	36.0	32.0	36.0
14	34.30943443614985	36.0	36.0	36.0	32.0	36.0
15	34.24943475421199	36.0	36.0	36.0	32.0	36.0
16	34.26573214889915	36.0	36.0	36.0	32.0	36.0
17	34.18854559503121	36.0	36.0	36.0	32.0	36.0
18	34.223143079159634	36.0	36.0	36.0	32.0	36.0
19	34.20256884537409	36.0	36.0	36.0	32.0	36.0
20	34.179852079166764	36.0	36.0	36.0	32.0	36.0
21	34.162278597214325	36.0	36.0	36.0	32.0	36.0
22	34.14296783788482	36.0	36.0	36.0	32.0	36.0
23	34.099891255649034	36.0	36.0	36.0	32.0	36.0
24	34.07884815438956	36.0	36.0	36.0	32.0	36.0
25	34.066938372168906	36.0	36.0	36.0	32.0	36.0
26	34.01210171627431	36.0	36.0	36.0	32.0	36.0
27	34.03038645099129	36.0	36.0	36.0	32.0	36.0
28	34.01094243459025	36.0	36.0	36.0	32.0	36.0
29	33.97249310819652	36.0	36.0	36.0	32.0	36.0
30	33.94599962819632	36.0	36.0	36.0	32.0	36.0
31	33.96584999640809	36.0	36.0	36.0	32.0	36.0
32	33.92563871540183	36.0	36.0	36.0	32.0	36.0
33	33.87371890873975	36.0	36.0	36.0	32.0	36.0
34	33.886446878411974	36.0	36.0	36.0	32.0	36.0
35	33.8471179456695	36.0	36.0	36.0	32.0	36.0
36	33.813385809931546	36.0	36.0	36.0	32.0	36.0
37	33.80817452618337	36.0	36.0	36.0	32.0	36.0
38	33.219457396946495	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	3.0
8	3.0
9	3.0
10	17.0
11	22.0
12	11.0
13	12.0
14	157.0
15	390.0
16	511.0
17	681.0
18	787.0
19	1162.0
20	1537.0
21	2420.0
22	3744.0
23	5635.0
24	8783.0
25	13142.0
26	19386.0
27	27264.0
28	37769.0
29	51084.0
30	66855.0
31	89480.0
32	120663.0
33	178865.0
34	394163.0
35	798993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41803816494217	17.532977727508573	11.462605110072174	25.586378997477084
2	16.708878956441964	19.464273368837016	38.00894798121736	25.81789969350366
3	19.16396467150466	23.56637036127028	28.564001088110636	28.705663879114418
4	12.268521565169323	15.449060486011485	36.551985855740845	35.73043209307835
5	14.432696842405432	36.64778398060914	33.81263915241782	15.106880024567598
6	34.018157041131644	36.204405180048646	16.74818415879048	13.02925362002923
7	30.320151485322803	30.568952857157743	20.555588763193413	18.555306894326044
8	27.994331894554374	33.2738518604229	19.205149114056912	19.526667130965812
9	27.30821770195208	14.193006017419973	18.743936927041318	39.754839353586625
10	15.878089735448341	26.484943006397454	31.62495105668212	26.012016201472083
11	37.61631452412498	21.33414568873992	22.30963025033123	18.739909536803868
12	24.840141530956057	23.964895847957322	28.793828092096025	22.401134528990593
13	29.296308748574667	19.630090546752324	25.459109714099547	25.61449099057346
14	23.443409471827586	20.18269728586597	25.28562904202389	31.088264200282556
15	25.152270119292886	27.665495020503805	22.453370018583975	24.72886484161933
16	25.55930816464874	25.95260085426397	24.122097882767957	24.365993098319333
17	23.770431108105306	26.13920277060608	25.322205273432253	24.768160847856365
18	24.528072171874356	25.197142739624606	26.57126012860414	23.703524959896896
19	25.277381233776815	24.943388507030576	25.799868706307414	23.979361552885198
20	25.508574499706597	24.644653208513624	25.228443098994752	24.618329192785026
21	27.081502979569137	24.217211851588612	24.653096547253565	24.048188621588686
22	25.704523704408533	24.557048801822972	25.390768319535812	24.347659174232682
23	24.40795463270701	24.47853938376825	25.934164774534647	25.179341208990095
24	24.739601472744198	25.258945089106728	25.596910593809014	24.404542844340067
25	24.81045803255904	24.782266013094482	25.707611461317427	24.699664493029058
26	24.15837109284369	25.9417572008177	26.223301178557463	23.676570527781145
27	24.905504331554205	25.11188111644192	25.449773056833415	24.532841495170466
28	24.142055513022726	25.267463261505252	26.148989705104892	24.441491520367133
29	24.115972360476512	25.129831061941033	26.036523264289368	24.717673313293087
30	24.010972408297746	25.504487840029217	26.160265820959122	24.324273930713915
31	24.84173995830918	25.38067140756698	24.994035942290953	24.783552691832888
32	24.300893167591497	25.467451428596497	24.983958521397962	25.247696882414044
33	24.142806451595213	25.076301317949305	25.600552385893536	25.180339844561946
34	24.886730166878646	25.121195019363228	25.955835048628835	24.03623976512929
35	25.32443777009057	25.075374431435183	25.748355529277422	23.851832269196827
36	23.991369206121632	25.66849436634473	25.326630244306976	25.013506183226664
37	25.158472043217156	25.549839580991033	24.99887569583459	24.292812679957223
38	24.184077563839956	25.410504506075647	25.557919236690037	24.847498693394364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	103.0
2	149.0
3	149.0
4	361.0
5	573.0
6	573.0
7	822.0
8	1071.0
9	1232.5
10	1394.0
11	1394.0
12	1940.5
13	2487.0
14	3470.5
15	4454.0
16	4454.0
17	6511.0
18	8568.0
19	8568.0
20	10452.5
21	12337.0
22	13808.0
23	15279.0
24	15279.0
25	18946.0
26	22613.0
27	22613.0
28	28789.5
29	34966.0
30	42086.0
31	49206.0
32	49206.0
33	60816.5
34	72427.0
35	72427.0
36	79025.0
37	85623.0
38	96542.0
39	107461.0
40	107461.0
41	112272.0
42	117083.0
43	130554.0
44	144025.0
45	144025.0
46	144110.0
47	144195.0
48	144195.0
49	152125.5
50	160056.0
51	162304.0
52	164552.0
53	164552.0
54	157367.5
55	150183.0
56	150183.0
57	143987.5
58	137792.0
59	123180.0
60	108568.0
61	108568.0
62	102622.5
63	96677.0
64	81203.0
65	65729.0
66	65729.0
67	55605.5
68	45482.0
69	45482.0
70	36534.0
71	27586.0
72	21399.0
73	15212.0
74	15212.0
75	11911.0
76	8610.0
77	8610.0
78	7882.5
79	7155.0
80	5648.0
81	4141.0
82	4141.0
83	3894.0
84	3647.0
85	3647.0
86	2518.0
87	1389.0
88	1162.5
89	936.0
90	936.0
91	677.0
92	418.0
93	366.5
94	315.0
95	315.0
96	294.5
97	274.0
98	274.0
99	563.5
100	853.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1227281177356388
2	0.05533184575302035
3	0.010912821907681914
4	0.0025774001490505025
5	1.645149031308831E-4
6	4.387064083490216E-4
7	0.0
8	3.290298062617662E-4
9	2.193532041745108E-4
10	2.741915052181385E-4
11	0.0021386937407014807
12	0.003016106557399524
13	0.015683754098477522
14	0.010912821907681914
15	0.025609486587374143
16	0.012009587928554468
17	0.022154673621625594
18	0.00647091952314807
19	0.008225745156544156
20	0.006196728017929931
21	0.006854787630453464
22	0.008116068554456901
23	0.011132175111856425
24	0.015354724292215757
25	0.0182063159464844
26	0.020948230998665787
27	0.00981605588680936
28	0.006306404620017187
29	0.011625719821249074
30	0.0032902980626176625
31	0.006580596125235325
32	0.007951553651326018
33	0.009870894187852986
34	0.013270868852557904
35	0.017109549925611846
36	0.016670843517262824
37	0.010583792101420148
38	0.006361242921060814
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1823543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.28825006808731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29884590005031	36.12771846423876
2	14.934886149841182	14.722288065835718
3	5.412585352017164	8.003305810352652
4	2.461408733448704	4.852741166959752
5	1.2602139523577154	3.1056870211549876
6	0.6846165917483064	2.02461322649113
7	0.44281436717153405	1.5277881684024683
8	0.2860927973304572	1.1280810670001755
9	0.18656332892077115	0.8275842008443615
>10	0.8494030396943482	7.298963502598712
>50	0.07879284818478946	2.773887737766793
>100	0.08577319824027788	9.2982243541925
>500	0.012524341561368519	4.089411106834655
>1k	0.005367574954872222	3.935416085101731
>5k	1.1182447822650461E-4	0.28429002222563415
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5158	0.2828559567830317	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3409	0.18694376825772685	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2368	0.12985709687131042	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2221	0.12179586661789715	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2189	0.12004104098450104	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2016	0.11055401490395345	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1913	0.1049056698964598	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1864	0.10221859314532204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1843	0.10106698882340587	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1839	0.10084763561923137	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1825	0.10007989940462056	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.483830104362771E-5
2	0.0	0.0	0.0	0.0	5.483830104362771E-5
3	0.0	0.0	0.0	0.0	5.483830104362771E-5
4	0.0	0.0	0.0	0.0	5.483830104362771E-5
5	0.0	0.0	0.0	0.0	5.483830104362771E-5
6	0.0	0.0	0.0	0.0	5.483830104362771E-5
7	0.0	0.0	0.0	5.483830104362771E-5	5.483830104362771E-5
8	0.0	0.0	0.0	5.483830104362771E-5	5.483830104362771E-5
9	0.0	0.0	0.0	1.0967660208725542E-4	5.483830104362771E-5
10	0.0	0.0	0.0	1.0967660208725542E-4	5.483830104362771E-5
11	0.0	0.0	0.0	1.0967660208725542E-4	5.483830104362771E-5
12	0.0	0.0	0.0	1.0967660208725542E-4	1.0967660208725542E-4
13	0.0	0.0	0.0	1.0967660208725542E-4	1.0967660208725542E-4
14	0.0	0.0	0.0	1.0967660208725542E-4	1.0967660208725542E-4
15	0.0	0.0	0.0	1.0967660208725542E-4	1.0967660208725542E-4
16	0.0	0.0	0.0	1.0967660208725542E-4	1.0967660208725542E-4
17	0.0	0.0	5.483830104362771E-5	1.645149031308831E-4	1.0967660208725542E-4
18	0.0	0.0	5.483830104362771E-5	1.645149031308831E-4	1.0967660208725542E-4
19	0.0	0.0	5.483830104362771E-5	2.1935320417451083E-4	1.0967660208725542E-4
20	0.0	0.0	5.483830104362771E-5	2.7419150521813856E-4	1.0967660208725542E-4
21	0.0	0.0	5.483830104362771E-5	2.7419150521813856E-4	2.1935320417451083E-4
22	0.0	0.0	5.483830104362771E-5	3.8386810730539395E-4	3.290298062617662E-4
23	0.0	0.0	5.483830104362771E-5	8.774128166980433E-4	3.290298062617662E-4
24	0.0	0.0	5.483830104362771E-5	0.0017548256333960867	3.290298062617662E-4
25	0.0	0.0	5.483830104362771E-5	0.0021386937407014807	3.290298062617662E-4
26	0.0	0.0	5.483830104362771E-5	0.0025225618480068743	3.290298062617662E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	75	3.2388707E-5	17.064518	5
TCTAGCG	400	0.0	16.399284	28
GATATAC	420	0.0	16.01863	1
TAGCGGC	410	0.0	15.9993	30
CTAGCGG	410	0.0	15.9993	29
AACCGCG	250	0.0	15.998423	7
ATAGCGT	365	0.0	15.778834	6
GTATTAG	445	0.0	15.478676	1
ATAAGGC	135	7.932613E-9	15.405467	3
GTCCTAA	325	0.0	15.279307	1
AATAGCG	370	0.0	15.133228	5
GTATCAA	3755	0.0	14.930812	1
TAGCGTA	365	0.0	14.902641	7
TAGGACG	395	0.0	14.580041	4
CGTATAT	375	0.0	14.506431	10
GTAGGAC	720	0.0	14.22043	3
AGCGTAT	385	0.0	14.128477	8
ACCCGTT	170	7.6033757E-10	14.117031	30
CAAGACG	580	0.0	14.066807	4
TTTTCGG	480	0.0	13.999388	29
>>END_MODULE