FastQCFastQC Report
Thu 2 Feb 2017
SRR4062835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823543
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT57640.3160879672154701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39970.2191886892713799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24360.1335861013422771No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23850.1307893479890521No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23730.13013128837652854No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21360.11713461102918878No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20430.11203464903213141No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20240.11099272131230248No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19380.10627662742255048No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19220.10539921460585246No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19180.10517986140167793No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18990.10413793368184902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT400.00448115820.0025546
CTAGCGG5000.017.59935229
TCTAGCG5100.017.25426528
TAGCGGC5200.016.92245330
CGCCGGT5750.016.1411917
CCGGTCC6300.016.0016049
CGGTCCA6000.016.00116510
ACTATAC1102.0206608E-716.0002883
CCTATAC1304.80577E-916.0002883
TAACCCG2050.015.60918128
GTATCAA38800.015.4641951
TGTAGGA6600.015.2725842
AACCCGT2100.015.23753429
ACCCGTT2100.015.23753430
ACCGTCG4650.015.1413038
ATCGTTT3500.015.08515829
GCCGGTC6300.014.985638
CGTCGTA4700.014.979815510
ATACCGT4750.014.8229466
CAAGACG6050.014.810594
Produced by FastQC (version 0.11.5)