##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1823543
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200443312825637	32.0	32.0	32.0	32.0	32.0
2	31.27859008534485	32.0	32.0	32.0	32.0	32.0
3	31.368658156127932	32.0	32.0	32.0	32.0	32.0
4	31.464338378639823	32.0	32.0	32.0	32.0	32.0
5	31.390542476925415	32.0	32.0	32.0	32.0	32.0
6	34.90190524709316	36.0	36.0	36.0	36.0	36.0
7	34.9276233135166	36.0	36.0	36.0	36.0	36.0
8	34.86150093526722	36.0	36.0	36.0	32.0	36.0
9	34.97523173295063	36.0	36.0	36.0	36.0	36.0
10	34.824057343314635	36.0	36.0	36.0	32.0	36.0
11	34.99617722203425	36.0	36.0	36.0	36.0	36.0
12	34.90369955630331	36.0	36.0	36.0	32.0	36.0
13	34.95714935156451	36.0	36.0	36.0	36.0	36.0
14	34.89731966835989	36.0	36.0	36.0	32.0	36.0
15	34.86869955904522	36.0	36.0	36.0	32.0	36.0
16	34.884467215744294	36.0	36.0	36.0	32.0	36.0
17	34.84497651001375	36.0	36.0	36.0	32.0	36.0
18	34.84929009077384	36.0	36.0	36.0	32.0	36.0
19	34.84075505759941	36.0	36.0	36.0	32.0	36.0
20	34.822927674313135	36.0	36.0	36.0	32.0	36.0
21	34.817734487204305	36.0	36.0	36.0	32.0	36.0
22	34.805795640684096	36.0	36.0	36.0	32.0	36.0
23	34.75286845443184	36.0	36.0	36.0	32.0	36.0
24	34.732771862248384	36.0	36.0	36.0	32.0	36.0
25	34.7106089628816	36.0	36.0	36.0	32.0	36.0
26	34.64865319874552	36.0	36.0	36.0	32.0	36.0
27	34.63931642960983	36.0	36.0	36.0	32.0	36.0
28	34.61338778411038	36.0	36.0	36.0	32.0	36.0
29	34.57978890544396	36.0	36.0	36.0	32.0	36.0
30	34.55640420872993	36.0	36.0	36.0	32.0	36.0
31	34.54655415309647	36.0	36.0	36.0	32.0	36.0
32	34.51912787359552	36.0	36.0	36.0	32.0	36.0
33	34.49130730671007	36.0	36.0	36.0	32.0	36.0
34	34.47697696188135	36.0	36.0	36.0	32.0	36.0
35	34.453276396553306	36.0	36.0	36.0	32.0	36.0
36	34.425162993140276	36.0	36.0	36.0	32.0	36.0
37	34.42107041073339	36.0	36.0	36.0	32.0	36.0
38	34.00321133090912	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	36.0
22	139.0
23	431.0
24	1207.0
25	2958.0
26	6315.0
27	12367.0
28	21723.0
29	33662.0
30	51107.0
31	73416.0
32	105488.0
33	168395.0
34	411239.0
35	935052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82102469041012	18.666048766576356	12.120073709265212	26.392852833748314
2	15.740292386853504	20.50069562384874	37.104033192526856	26.6549787967709
3	18.156522769136785	24.289419004651933	28.881249304239056	28.672808921972226
4	11.743202781403204	16.370301720828717	36.495552606468735	35.390942891299346
5	13.901023392935288	37.16953050711198	33.460375467085484	15.469070632867243
6	34.05880869078825	36.527304035008825	16.4440593570747	12.969827917128224
7	29.912933038448863	30.785247806600243	20.61875287558972	18.683066279361178
8	27.8469246402785	33.53759321390693	18.995017258077077	19.620464887737494
9	27.02810901401283	14.048364877552274	18.61443444154887	40.309091666886026
10	15.61164844095742	26.81912978339182	31.632943483575477	25.936278292075286
11	37.62117127294823	21.33607026672487	22.17217672853467	18.870581731792228
12	24.520066793343698	24.091119720762148	28.753821560869408	22.634991925024746
13	29.272984714330054	19.636168810301733	25.470321978483586	25.620524496884627
14	23.281681080929907	20.1821072298347	25.233323517266683	31.30288817196871
15	25.01602649348	27.76435762688349	22.339917402550967	24.87969847708554
16	25.554703124631555	26.039857573964532	23.93472487350175	24.470714427902166
17	23.672817147717385	26.298858869793584	25.22720879079901	24.801115191690023
18	24.521768886173785	25.18037688170775	26.593230869795775	23.70462336232269
19	25.22978619094806	24.93333033550621	25.80207870063936	24.03480477290637
20	25.491364886926167	24.616913338484476	25.208070223734786	24.683651550854574
21	26.977183403060312	24.298987519337377	24.65291430244782	24.070914775154492
22	25.581368567326663	24.58725930085515	25.519456091496657	24.311916040321528
23	24.317041177141554	24.454192294531065	25.986764176852006	25.242002351475374
24	24.691712781107984	25.23439260823573	25.59605120361845	24.477843407037838
25	24.75193880919661	24.72561641026091	25.696145194352532	24.826299586189954
26	24.16817152104447	25.903913425677377	26.18139522895813	23.74651982432002
27	24.954278566504875	25.031984439083693	25.403404252052187	24.61033274235924
28	24.077468971118314	25.181693000932796	26.212269192445696	24.528568835503194
29	24.107136491982914	24.96535590331569	26.120360199896574	24.807147404804823
30	24.06672066411376	25.38124957843056	26.178982343712214	24.373047413743464
31	24.861163131332795	25.15081903744524	25.11511930346584	24.87289852775613
32	24.333344483787876	25.313304923437506	25.112487065015742	25.240863527758872
33	24.083556022534154	24.949014089604688	25.695747234915768	25.271682652945394
34	24.825463397353396	25.002262079918047	25.99220309035762	24.18007143237094
35	25.26663752924938	24.997655662630386	25.855491205855852	23.880215602264382
36	23.980350339970048	25.63509607396151	25.41267192492856	24.97188166113988
37	25.192989690947787	25.302666293035042	25.119890235656634	24.38445378036054
38	24.20107450167065	25.14829647559723	25.678966714796413	24.971662307935706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	49.0
2	65.0
3	65.0
4	255.0
5	445.0
6	445.0
7	695.0
8	945.0
9	1194.5
10	1444.0
11	1444.0
12	2001.5
13	2559.0
14	3602.0
15	4645.0
16	4645.0
17	6790.0
18	8935.0
19	8935.0
20	10805.5
21	12676.0
22	14104.5
23	15533.0
24	15533.0
25	18937.5
26	22342.0
27	22342.0
28	28820.5
29	35299.0
30	42309.5
31	49320.0
32	49320.0
33	61070.0
34	72820.0
35	72820.0
36	79175.0
37	85530.0
38	96454.0
39	107378.0
40	107378.0
41	112819.5
42	118261.0
43	131141.5
44	144022.0
45	144022.0
46	144076.0
47	144130.0
48	144130.0
49	152060.0
50	159990.0
51	161809.5
52	163629.0
53	163629.0
54	157088.0
55	150547.0
56	150547.0
57	144157.0
58	137767.0
59	123659.0
60	109551.0
61	109551.0
62	103322.0
63	97093.0
64	81276.0
65	65459.0
66	65459.0
67	55774.5
68	46090.0
69	46090.0
70	36910.5
71	27731.0
72	21406.0
73	15081.0
74	15081.0
75	11652.5
76	8224.0
77	8224.0
78	7635.5
79	7047.0
80	5515.5
81	3984.0
82	3984.0
83	3630.0
84	3276.0
85	3276.0
86	2123.5
87	971.0
88	758.0
89	545.0
90	545.0
91	322.0
92	99.0
93	73.5
94	48.0
95	48.0
96	33.0
97	18.0
98	18.0
99	14.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008938643070111316
2	0.0
3	0.0
4	1.645149031308831E-4
5	5.48383010436277E-5
6	1.645149031308831E-4
7	2.193532041745108E-4
8	0.0026870767511377575
9	0.0038935193740975674
10	0.001096766020872554
11	0.004990285394970121
12	4.387064083490216E-4
13	2.741915052181385E-4
14	1.096766020872554E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.096766020872554E-4
22	5.48383010436277E-5
23	3.8386810730539395E-4
24	0.0
25	5.48383010436277E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1823543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.851529725046326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8657573653176	33.20169137689098
2	16.07739375672013	15.065009829884948
3	6.032884369646868	8.479495841168328
4	2.713920179494395	5.086052478439387
5	1.3924751809601974	3.2619796166072974
6	0.7699254053286692	2.164330980829469
7	0.4889742986818652	1.6036435722633962
8	0.3058827089699023	1.1464858265344855
9	0.23083879942386704	0.9733635785611183
>10	0.9234383239006896	7.54697619870941
>50	0.08426094775700557	2.7982254563949063
>100	0.09363748239130196	9.536778231889393
>500	0.014486716075036852	4.497903789882047
>1k	0.006006687153064061	4.3200006367441075
>5k	1.1777817947184434E-4	0.3180625852007081
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5764	0.3160879672154701	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3997	0.2191886892713799	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2436	0.1335861013422771	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2385	0.1307893479890521	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2373	0.13013128837652854	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2136	0.11713461102918878	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2043	0.11203464903213141	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2024	0.11099272131230248	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1938	0.10627662742255048	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1922	0.10539921460585246	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1918	0.10517986140167793	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1899	0.10413793368184902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.483830104362771E-5
13	0.0	0.0	0.0	0.0	5.483830104362771E-5
14	0.0	0.0	0.0	0.0	5.483830104362771E-5
15	0.0	0.0	0.0	0.0	1.645149031308831E-4
16	0.0	0.0	0.0	0.0	2.1935320417451083E-4
17	0.0	0.0	0.0	0.0	2.1935320417451083E-4
18	0.0	0.0	0.0	5.483830104362771E-5	2.1935320417451083E-4
19	0.0	0.0	0.0	1.0967660208725542E-4	2.1935320417451083E-4
20	0.0	0.0	0.0	1.0967660208725542E-4	2.7419150521813856E-4
21	0.0	0.0	0.0	1.0967660208725542E-4	3.290298062617662E-4
22	0.0	0.0	0.0	1.645149031308831E-4	3.290298062617662E-4
23	0.0	0.0	0.0	6.032213114799047E-4	3.290298062617662E-4
24	0.0	0.0	0.0	0.0014257958271343203	3.290298062617662E-4
25	0.0	0.0	0.0	0.0018096639344397144	3.290298062617662E-4
26	0.0	0.0	0.0	0.0021935320417451084	3.290298062617662E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCGT	40	0.004481158	20.002554	6
CTAGCGG	500	0.0	17.599352	29
TCTAGCG	510	0.0	17.254265	28
TAGCGGC	520	0.0	16.922453	30
CGCCGGT	575	0.0	16.141191	7
CCGGTCC	630	0.0	16.001604	9
CGGTCCA	600	0.0	16.001165	10
ACTATAC	110	2.0206608E-7	16.000288	3
CCTATAC	130	4.80577E-9	16.000288	3
TAACCCG	205	0.0	15.609181	28
GTATCAA	3880	0.0	15.464195	1
TGTAGGA	660	0.0	15.272584	2
AACCCGT	210	0.0	15.237534	29
ACCCGTT	210	0.0	15.237534	30
ACCGTCG	465	0.0	15.141303	8
ATCGTTT	350	0.0	15.085158	29
GCCGGTC	630	0.0	14.98563	8
CGTCGTA	470	0.0	14.9798155	10
ATACCGT	475	0.0	14.822946	6
CAAGACG	605	0.0	14.81059	4
>>END_MODULE