FastQCFastQC Report
Thu 2 Feb 2017
SRR4062834_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062834_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997669
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41180.4127621485683127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28260.2832602797120087No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15980.16017336411174446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15650.15686565383909895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.13020350436868341No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12810.1283992987654222No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12730.12759742960841722No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12380.12408925204652044No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12160.12188411186475676No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11810.11837593430285996No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11580.11607056047647066No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11450.11476752309633757No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11430.11456705580708633No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10850.10875350441880022No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10670.10694929881553902No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10420.10444345769989848No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10260.1028397193858885No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10250.10273948574126288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10150.10173714929500666No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10000.10023364462562233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT659.452331E-619.6873426
TCTAGCG2700.018.36942128
TAGTAGA450.00886234217.7732964
GTTATTC1950.017.2264233
CTAGCGG2900.017.10342229
AGGCCCG3400.016.9428510
TGCTATA1051.1872544E-716.763562
TAGCGGC3000.016.53330830
CGAGCCG3350.016.2404115
ACGAACG1102.0161133E-716.0015815
ACCATGC1407.421477E-1015.9983718
CACGAAT3350.015.76037927
GTCCTAA2650.015.7083241
GAGCCGC3650.015.34321116
TAACGAA1153.339701E-715.30585913
TATTCCC2200.015.2688765
GCATTCG3150.015.23807122
AGAACCG2000.015.1961675
ATCACGA3500.015.08569125
CGGTCCA3100.014.96921910
Produced by FastQC (version 0.11.5)