##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062834_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997669
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.194234761228422	32.0	32.0	32.0	32.0	32.0
2	30.822494234059594	32.0	32.0	32.0	32.0	32.0
3	30.88103469186674	32.0	32.0	32.0	32.0	32.0
4	30.933300523520327	32.0	32.0	32.0	32.0	32.0
5	30.881095834389964	32.0	32.0	32.0	32.0	32.0
6	34.52234057588238	36.0	36.0	36.0	32.0	36.0
7	34.427253928908286	36.0	36.0	36.0	32.0	36.0
8	34.408683641568494	36.0	36.0	36.0	32.0	36.0
9	34.52547087260404	36.0	36.0	36.0	32.0	36.0
10	34.27997061149539	36.0	36.0	36.0	32.0	36.0
11	34.55018648469583	36.0	36.0	36.0	32.0	36.0
12	34.35670648281143	36.0	36.0	36.0	32.0	36.0
13	34.44908281203485	36.0	36.0	36.0	32.0	36.0
14	34.352419489830794	36.0	36.0	36.0	32.0	36.0
15	34.31258563712013	36.0	36.0	36.0	32.0	36.0
16	34.3120303427289	36.0	36.0	36.0	32.0	36.0
17	34.247860763439576	36.0	36.0	36.0	32.0	36.0
18	34.26060948069951	36.0	36.0	36.0	32.0	36.0
19	34.25446415594752	36.0	36.0	36.0	32.0	36.0
20	34.22095805322206	36.0	36.0	36.0	32.0	36.0
21	34.19662132430696	36.0	36.0	36.0	32.0	36.0
22	34.17765210706156	36.0	36.0	36.0	32.0	36.0
23	34.12302878008638	36.0	36.0	36.0	32.0	36.0
24	34.11945745532837	36.0	36.0	36.0	32.0	36.0
25	34.089632934370016	36.0	36.0	36.0	32.0	36.0
26	34.04387026157974	36.0	36.0	36.0	32.0	36.0
27	34.048673457830205	36.0	36.0	36.0	32.0	36.0
28	34.02916097423093	36.0	36.0	36.0	32.0	36.0
29	33.996878724306356	36.0	36.0	36.0	32.0	36.0
30	33.963191198684136	36.0	36.0	36.0	32.0	36.0
31	33.98680023133925	36.0	36.0	36.0	32.0	36.0
32	33.917159899726265	36.0	36.0	36.0	32.0	36.0
33	33.87267921525075	36.0	36.0	36.0	32.0	36.0
34	33.88505706802557	36.0	36.0	36.0	32.0	36.0
35	33.83377352608932	36.0	36.0	36.0	32.0	36.0
36	33.78816220610243	36.0	36.0	36.0	32.0	36.0
37	33.77595475052347	36.0	36.0	36.0	32.0	36.0
38	33.17322578931489	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	8.0
12	6.0
13	13.0
14	173.0
15	343.0
16	443.0
17	513.0
18	611.0
19	791.0
20	1072.0
21	1514.0
22	2279.0
23	3160.0
24	4753.0
25	7104.0
26	10022.0
27	14286.0
28	19849.0
29	26760.0
30	35753.0
31	47556.0
32	64191.0
33	95019.0
34	210522.0
35	450917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.94981906201266	17.56623855715252	11.467735234436624	25.0162071463982
2	16.130889222739935	20.315850280508425	38.10692415541591	25.44633634133573
3	18.603837552114502	25.180063815518416	28.74545266815229	27.47064596421479
4	12.22309761798798	16.058934850507796	36.57296590767849	35.145001623825735
5	14.160636765574083	37.213017970926174	33.58264831852713	15.04369694497262
6	32.84729844186175	36.26558012960262	17.55859932943423	13.328522099101402
7	29.33878871649816	31.199425861683583	21.49189761333669	17.969887808481573
8	27.838792224675718	33.02157328733277	20.056952756876278	19.08268173111523
9	27.781258112660613	14.289107910539467	19.097917245098326	38.8317167317016
10	15.939402565588082	26.916122229283147	31.710011166061587	25.434464039067183
11	36.64196204295277	21.62152409552811	22.80800758579628	18.928506275722846
12	24.99626620691729	24.292107914137794	29.040791062953254	21.67083481599166
13	29.530453646858795	19.942437013541635	25.48741072824897	25.039698611350598
14	23.66510620808565	20.572992371462654	25.730424932586182	30.03147648786551
15	24.884752892958044	27.740715564725864	23.17935638074957	24.195175161566517
16	24.981078737513222	25.963781445634577	24.932159129070577	24.122980687781627
17	23.30762467429739	26.160273254339277	26.10342881261285	24.428673258750486
18	24.34503857195554	24.767746307196354	27.69231848727366	23.19489663357444
19	25.153970761593918	25.19176176232368	26.33521519819726	23.319052277885145
20	25.34702870454646	24.30183277667069	26.177912855899887	24.173225662882963
21	26.75523932963044	24.395874494661683	24.88514924333324	23.963736932374633
22	25.3160864522635	24.9747642069746	25.565487258806463	24.14366208195543
23	23.652718021099396	24.783372294910404	26.300768672486175	25.263141011504025
24	24.27104364615517	25.646752653074316	26.011356140516202	24.07084756025431
25	24.44154828619836	24.966365577599774	26.04637640477599	24.54570973142588
26	23.73441928404649	26.063017623640683	26.724292633239997	23.47827045907283
27	24.809712410870826	25.476232721746566	25.772051858066963	23.942003009315645
28	23.612716328443337	25.36629862221398	26.546914390822113	24.47407065852057
29	23.722132724369224	25.433942063534232	26.504173763283916	24.33975144881263
30	23.693593186326545	25.603899599154406	26.822875162508357	23.879632052010695
31	24.431462214929187	25.541122069600835	25.434265935447264	24.593149780022713
32	23.71053415035841	25.874020026002327	25.36109053401002	25.054355289629243
33	23.39499248682563	25.23544454034309	26.347735171379565	25.021827801451714
34	24.28676688036009	25.67067822181678	26.36529044093691	23.677264456886224
35	25.141703976533382	25.364059420432238	26.203456233671712	23.29078036936267
36	23.71266825662726	26.13269654709882	25.926984117436536	24.227651078837383
37	24.710696413678065	25.94720359915314	25.590235452620774	23.75186453454802
38	23.697603154761964	25.979272315740833	26.020471069152762	24.302653460344445
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1182.0
1	815.5
2	449.0
3	449.0
4	957.0
5	1465.0
6	1465.0
7	1595.0
8	1725.0
9	1607.5
10	1490.0
11	1490.0
12	1943.0
13	2396.0
14	2903.0
15	3410.0
16	3410.0
17	4961.0
18	6512.0
19	6512.0
20	7640.5
21	8769.0
22	8962.0
23	9155.0
24	9155.0
25	10638.5
26	12122.0
27	12122.0
28	15665.0
29	19208.0
30	23821.5
31	28435.0
32	28435.0
33	34844.5
34	41254.0
35	41254.0
36	44653.5
37	48053.0
38	53667.0
39	59281.0
40	59281.0
41	61490.0
42	63699.0
43	70954.0
44	78209.0
45	78209.0
46	81118.0
47	84027.0
48	84027.0
49	86822.0
50	89617.0
51	88783.0
52	87949.0
53	87949.0
54	82699.0
55	77449.0
56	77449.0
57	75193.0
58	72937.0
59	64517.5
60	56098.0
61	56098.0
62	53677.0
63	51256.0
64	42364.0
65	33472.0
66	33472.0
67	27717.0
68	21962.0
69	21962.0
70	17801.5
71	13641.0
72	10633.5
73	7626.0
74	7626.0
75	5759.0
76	3892.0
77	3892.0
78	3924.5
79	3957.0
80	3058.0
81	2159.0
82	2159.0
83	2253.5
84	2348.0
85	2348.0
86	1578.5
87	809.0
88	719.0
89	629.0
90	629.0
91	431.5
92	234.0
93	202.0
94	170.0
95	170.0
96	164.0
97	158.0
98	158.0
99	311.5
100	465.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11977920532761868
2	0.0552287381887179
3	0.010624766330315966
4	0.0025058411156405583
5	2.0046728925124464E-4
6	4.009345785024893E-4
7	0.0
8	0.0
9	0.0
10	3.0070093387686694E-4
11	0.001503504669384335
12	0.0024056074710149355
13	0.015335747627720216
14	0.00992313081793661
15	0.024757710222528713
16	0.011426635487320945
17	0.02104906537138069
18	0.005312383165157983
19	0.007717990636172919
20	0.0057133177436604725
21	0.007016355123793562
22	0.007818224280798543
23	0.010524532685690344
24	0.014734345759966481
25	0.017941822387986396
26	0.02024719621437571
27	0.010023364462562233
28	0.005412616809783606
29	0.012829906512079657
30	0.002806542049517425
31	0.006615420545291074
32	0.006615420545291074
33	0.008820560727054765
34	0.013431308379833392
35	0.016739018652478926
36	0.016037383140099573
37	0.010123598107187855
38	0.006615420545291074
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	997669.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.047685348011235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43268060870564	46.455459404105355
2	12.194134658539815	13.912943141833154
3	3.151511456368148	5.393593018006282
4	1.1591711711761394	2.645121289509683
5	0.55885974891168	1.5940827554791042
6	0.3143676785712184	1.0760369046429359
7	0.19011351253982206	0.7591875090643869
8	0.13848362818316196	0.6320136357155202
9	0.10326001256405061	0.5301670235207082
>10	0.5673528981421623	6.325458370908116
>50	0.08518199739161238	3.4520320998457565
>100	0.09256419951229103	11.06129516860058
>500	0.008974855701561136	3.4218315397782026
>1k	0.003343573692738462	2.7407781389903096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4118	0.4127621485683127	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2826	0.2832602797120087	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1598	0.16017336411174446	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1565	0.15686565383909895	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1299	0.13020350436868341	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1281	0.1283992987654222	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1273	0.12759742960841722	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1238	0.12408925204652044	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1216	0.12188411186475676	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1181	0.11837593430285996	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1158	0.11607056047647066	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1145	0.11476752309633757	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1143	0.11456705580708633	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1085	0.10875350441880022	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1067	0.10694929881553902	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1042	0.10444345769989848	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1026	0.1028397193858885	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1025	0.10273948574126288	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1015	0.10173714929500666	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1000	0.10023364462562233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0023364462562233E-4	0.0
8	0.0	0.0	0.0	1.0023364462562233E-4	0.0
9	0.0	0.0	0.0	1.0023364462562233E-4	0.0
10	0.0	0.0	0.0	4.0093457850248933E-4	0.0
11	0.0	0.0	0.0	5.011682231281117E-4	0.0
12	0.0	0.0	0.0	5.011682231281117E-4	0.0
13	0.0	0.0	0.0	6.01401867753734E-4	0.0
14	0.0	0.0	0.0	6.01401867753734E-4	0.0
15	0.0	0.0	0.0	7.016355123793563E-4	0.0
16	0.0	0.0	0.0	7.016355123793563E-4	0.0
17	0.0	0.0	0.0	7.016355123793563E-4	0.0
18	0.0	0.0	0.0	7.016355123793563E-4	0.0
19	0.0	0.0	0.0	8.018691570049787E-4	0.0
20	0.0	0.0	0.0	0.0011025700908818457	0.0
21	0.0	0.0	0.0	0.0013030373801330903	0.0
22	0.0	0.0	0.0	0.002104906537138069	0.0
23	0.0	0.0	0.0	0.003808878495773648	0.0
24	0.0	0.0	0.0	0.0057133177436604725	0.0
25	0.0	0.0	0.0	0.006615420545291074	0.0
26	0.0	0.0	0.0	0.009021028016306009	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGCGT	65	9.452331E-6	19.687342	6
TCTAGCG	270	0.0	18.369421	28
TAGTAGA	45	0.008862342	17.773296	4
GTTATTC	195	0.0	17.226423	3
CTAGCGG	290	0.0	17.103422	29
AGGCCCG	340	0.0	16.94285	10
TGCTATA	105	1.1872544E-7	16.76356	2
TAGCGGC	300	0.0	16.533308	30
CGAGCCG	335	0.0	16.24041	15
ACGAACG	110	2.0161133E-7	16.00158	15
ACCATGC	140	7.421477E-10	15.998371	8
CACGAAT	335	0.0	15.760379	27
GTCCTAA	265	0.0	15.708324	1
GAGCCGC	365	0.0	15.343211	16
TAACGAA	115	3.339701E-7	15.305859	13
TATTCCC	220	0.0	15.268876	5
GCATTCG	315	0.0	15.238071	22
AGAACCG	200	0.0	15.196167	5
ATCACGA	350	0.0	15.085691	25
CGGTCCA	310	0.0	14.969219	10
>>END_MODULE