FastQCFastQC Report
Thu 2 Feb 2017
SRR4062834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997669
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49390.49505397080594865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38030.3811885505112417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22340.22392196209364026No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16440.1647841117645231No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.16287967251663626No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14700.14734345759966483No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14010.14042733612049688No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12900.1293014015670528No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12180.122084579154008No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12130.12158341093087988No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11940.11967897168299307No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11770.11797499972435747No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11580.11607056047647066No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11400.11426635487320944No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10930.1095553735758052No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10880.10905420535267708No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10840.1086532707741746No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10360.10384205583214472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA502.7977736E-522.39933411
ACCGTCG2600.020.3081078
TACCGTC2700.019.5559547
CGTCGTA2750.019.19942910
ATACCGT3100.018.0648846
CCGTCGT2950.017.898679
TAGCGGC2900.017.65464630
CTAGCGG2900.017.65464629
GGTTCGT550.001364746517.45402726
GTCGTAG3150.017.26932711
CATCGTT2100.016.76140628
GCGCAAG2700.016.5962581
ATCGTTT2150.016.37160529
TCTAGAT2250.016.3550682
CGTATAT2450.016.32604610
ATAGCGT2400.016.0003266
AAGACGG3000.016.0003265
CAAGACG3000.016.0003264
AATAGCG2400.016.0003265
TAGTGCT600.002441271216.0003264
Produced by FastQC (version 0.11.5)