##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062834_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997669
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.157589340753297	32.0	32.0	32.0	32.0	32.0
2	31.309160653483268	32.0	32.0	32.0	32.0	32.0
3	31.394158784125796	32.0	32.0	32.0	32.0	32.0
4	31.483564188122514	32.0	32.0	32.0	32.0	32.0
5	31.428491814419413	32.0	32.0	32.0	32.0	32.0
6	34.93067640670403	36.0	36.0	36.0	36.0	36.0
7	34.96139801878178	36.0	36.0	36.0	36.0	36.0
8	34.90290567312405	36.0	36.0	36.0	36.0	36.0
9	35.01455492753609	36.0	36.0	36.0	36.0	36.0
10	34.8682619185321	36.0	36.0	36.0	32.0	36.0
11	35.021131256959976	36.0	36.0	36.0	36.0	36.0
12	34.929482623996535	36.0	36.0	36.0	32.0	36.0
13	34.97444944164848	36.0	36.0	36.0	36.0	36.0
14	34.91338309599677	36.0	36.0	36.0	32.0	36.0
15	34.89243927595224	36.0	36.0	36.0	32.0	36.0
16	34.89395881800477	36.0	36.0	36.0	32.0	36.0
17	34.86519176199722	36.0	36.0	36.0	32.0	36.0
18	34.85899431575001	36.0	36.0	36.0	32.0	36.0
19	34.852707661559094	36.0	36.0	36.0	32.0	36.0
20	34.839772509720156	36.0	36.0	36.0	32.0	36.0
21	34.823441442001304	36.0	36.0	36.0	32.0	36.0
22	34.812471871933475	36.0	36.0	36.0	32.0	36.0
23	34.73692777865204	36.0	36.0	36.0	32.0	36.0
24	34.7238583137293	36.0	36.0	36.0	32.0	36.0
25	34.71048313619046	36.0	36.0	36.0	32.0	36.0
26	34.64441813868127	36.0	36.0	36.0	32.0	36.0
27	34.62297214807717	36.0	36.0	36.0	32.0	36.0
28	34.589674531332534	36.0	36.0	36.0	32.0	36.0
29	34.54543440760413	36.0	36.0	36.0	32.0	36.0
30	34.52459382821357	36.0	36.0	36.0	32.0	36.0
31	34.51521897543173	36.0	36.0	36.0	32.0	36.0
32	34.46174833536975	36.0	36.0	36.0	32.0	36.0
33	34.418837309769074	36.0	36.0	36.0	32.0	36.0
34	34.40431646167216	36.0	36.0	36.0	32.0	36.0
35	34.352471611326	36.0	36.0	36.0	32.0	36.0
36	34.319807471215405	36.0	36.0	36.0	32.0	36.0
37	34.31000161376168	36.0	36.0	36.0	32.0	36.0
38	33.892784079689754	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	7.0
21	28.0
22	82.0
23	257.0
24	710.0
25	1674.0
26	3674.0
27	6927.0
28	12153.0
29	19067.0
30	28565.0
31	40718.0
32	57727.0
33	90988.0
34	219913.0
35	515170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8030975114653	18.856827807433024	12.377615718116433	25.96245896298524
2	15.290642487638687	21.324607660456525	37.53800108051869	25.846748771386103
3	17.58088103368953	25.817981715378547	29.29919642687104	27.30194082406089
4	11.742795942934867	16.840488860511574	36.5632019501497	34.853513246403864
5	13.53875884687206	37.781869537892824	33.33309945482921	15.346272160405904
6	32.835339175618365	36.69824360584523	17.147570987972966	13.318846230563445
7	28.978092824838825	31.317357346762037	21.505837636719377	18.198712191679764
8	27.800158171862048	33.11605584729699	19.91071007939666	19.173075901444296
9	27.607744470562288	14.052615487938732	18.94628689424277	39.39335314725621
10	15.713168815027165	27.143315357937574	31.631517751538603	25.51199807549666
11	36.79978348469357	21.42609410396744	22.7929472143702	18.981175196968785
12	24.71448360473345	24.45547908819184	29.06794458265592	21.762092724418792
13	29.69016715998424	19.75649189559242	25.530462569173885	25.02287837524946
14	23.4428452723298	20.48825812970033	25.797233350941045	30.271663247028822
15	24.82627003545264	27.771535449131928	23.174118871088506	24.22807564432693
16	24.956874474399825	25.913003210483637	24.827172138254273	24.302950176862264
17	23.208098076616594	26.186240125733086	26.133316761370757	24.472345036279567
18	24.34905765339005	24.54832213890579	27.849416991006038	23.25320321669812
19	25.136994333792067	25.17588498790681	26.428003676570082	23.259117001731035
20	25.32393008101885	24.184874943493284	26.36926676081947	24.121928214668394
21	26.68263062514033	24.38656702056003	25.14864723995253	23.782155114347113
22	25.12115741794122	24.903550175458992	25.959411387945302	24.015881018654483
23	23.4184352093434	24.472394095424626	26.648771584105717	25.460399111126257
24	24.188984522922933	25.4394994732722	26.350422835629857	24.021093168175017
25	24.33442354127471	24.676621204026585	26.352728209456245	24.63622704524246
26	23.67458545870424	25.70321419228221	27.05205834800921	23.570142001004342
27	24.82837494198978	25.107124707693636	25.902278210508694	24.16222213980789
28	23.59810718785489	25.078157184396826	26.8251293765768	24.49860625117148
29	23.72770929035582	25.088481249793272	26.810395030816835	24.373414429034078
30	23.70666022498444	25.307992931523383	27.062783347984148	23.92256349550803
31	24.378025176686858	25.052497371372667	25.804951341577215	24.764526110363256
32	23.726005318397185	25.453832884453657	25.87010321058387	24.950058586565284
33	23.352534758522115	24.867866997972275	26.687909517084325	25.09168872642129
34	24.22256279387252	25.192223071980784	26.880859282988645	23.70435485115805
35	25.231915595252534	25.04568148353813	26.400038489719535	23.322364431489802
36	23.836162093840745	25.7579417622478	26.121188490371054	24.2847076535404
37	24.71944574748313	25.70464322800771	25.7592706190837	23.816640405425453
38	23.829670792287615	25.49966521929139	26.31667047554898	24.35399351287202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	695.0
1	479.0
2	263.0
3	263.0
4	824.0
5	1385.0
6	1385.0
7	1555.5
8	1726.0
9	1667.5
10	1609.0
11	1609.0
12	2018.0
13	2427.0
14	3037.5
15	3648.0
16	3648.0
17	5571.0
18	7494.0
19	7494.0
20	8390.5
21	9287.0
22	9391.0
23	9495.0
24	9495.0
25	10722.0
26	11949.0
27	11949.0
28	15564.0
29	19179.0
30	23610.5
31	28042.0
32	28042.0
33	34743.0
34	41444.0
35	41444.0
36	44779.5
37	48115.0
38	53722.0
39	59329.0
40	59329.0
41	61364.0
42	63399.0
43	70750.0
44	78101.0
45	78101.0
46	81098.0
47	84095.0
48	84095.0
49	87490.5
50	90886.0
51	89320.5
52	87755.0
53	87755.0
54	82653.0
55	77551.0
56	77551.0
57	75370.0
58	73189.0
59	64749.0
60	56309.0
61	56309.0
62	53600.5
63	50892.0
64	42157.5
65	33423.0
66	33423.0
67	27870.0
68	22317.0
69	22317.0
70	17943.5
71	13570.0
72	10575.5
73	7581.0
74	7581.0
75	5620.5
76	3660.0
77	3660.0
78	3721.5
79	3783.0
80	2965.5
81	2148.0
82	2148.0
83	2083.0
84	2018.0
85	2018.0
86	1235.5
87	453.0
88	395.0
89	337.0
90	337.0
91	203.0
92	69.0
93	47.5
94	26.0
95	26.0
96	17.5
97	9.0
98	9.0
99	10.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009421962594808498
2	0.0
3	0.0
4	2.0046728925124464E-4
5	0.0
6	0.0
7	5.011682231281117E-4
8	0.0020046728925124466
9	0.005412616809783606
10	9.02102801630601E-4
11	0.004911448586655494
12	3.0070093387686694E-4
13	2.0046728925124464E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	5.011682231281117E-4
22	0.0
23	2.0046728925124464E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	1.0023364462562232E-4
38	1.0023364462562232E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	997669.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.164662382925876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22604861941494	44.256428864062215
2	12.908721375541333	14.24210512953992
3	3.4120506142499387	5.646738605056583
4	1.2978202362773266	2.8637526067187125
5	0.5753961180612286	1.5870766294646916
6	0.33402451064539046	1.1055809614424996
7	0.20638890361195536	0.7969761931135024
8	0.141381706436218	0.6239419282140723
9	0.10281467666299114	0.5104563233511235
>10	0.5939350062956101	6.454560875051076
>50	0.08775974385382596	3.436411571787865
>100	0.098698623774487	11.230525330716077
>500	0.011675992331526146	4.245639742597015
>1k	0.0032838728432417284	2.9998052388846825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4939	0.49505397080594865	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3803	0.3811885505112417	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2234	0.22392196209364026	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1644	0.1647841117645231	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1625	0.16287967251663626	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1470	0.14734345759966483	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1401	0.14042733612049688	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1290	0.1293014015670528	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1218	0.122084579154008	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1213	0.12158341093087988	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1194	0.11967897168299307	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1177	0.11797499972435747	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1158	0.11607056047647066	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1140	0.11426635487320944	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1093	0.1095553735758052	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1088	0.10905420535267708	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1084	0.1086532707741746	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1036	0.10384205583214472	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0023364462562233E-4	0.0
8	0.0	0.0	0.0	1.0023364462562233E-4	0.0
9	0.0	0.0	0.0	1.0023364462562233E-4	0.0
10	0.0	0.0	0.0	1.0023364462562233E-4	0.0
11	0.0	0.0	0.0	2.0046728925124466E-4	0.0
12	0.0	0.0	0.0	2.0046728925124466E-4	1.0023364462562233E-4
13	0.0	0.0	0.0	3.00700933876867E-4	1.0023364462562233E-4
14	0.0	0.0	0.0	3.00700933876867E-4	1.0023364462562233E-4
15	0.0	0.0	0.0	4.0093457850248933E-4	1.0023364462562233E-4
16	0.0	0.0	0.0	4.0093457850248933E-4	1.0023364462562233E-4
17	0.0	0.0	0.0	4.0093457850248933E-4	1.0023364462562233E-4
18	0.0	0.0	0.0	5.011682231281117E-4	1.0023364462562233E-4
19	0.0	0.0	0.0	6.01401867753734E-4	1.0023364462562233E-4
20	0.0	0.0	0.0	9.021028016306009E-4	2.0046728925124466E-4
21	0.0	0.0	0.0	0.0011025700908818457	2.0046728925124466E-4
22	0.0	0.0	0.0	0.001904439247886824	2.0046728925124466E-4
23	0.0	0.0	0.0	0.003808878495773648	2.0046728925124466E-4
24	0.0	0.0	0.0	0.0057133177436604725	2.0046728925124466E-4
25	0.0	0.0	0.0	0.006615420545291074	2.0046728925124466E-4
26	0.0	0.0	0.0	0.008920794371680387	2.0046728925124466E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTGAA	50	2.7977736E-5	22.399334	11
ACCGTCG	260	0.0	20.308107	8
TACCGTC	270	0.0	19.555954	7
CGTCGTA	275	0.0	19.199429	10
ATACCGT	310	0.0	18.064884	6
CCGTCGT	295	0.0	17.89867	9
TAGCGGC	290	0.0	17.654646	30
CTAGCGG	290	0.0	17.654646	29
GGTTCGT	55	0.0013647465	17.454027	26
GTCGTAG	315	0.0	17.269327	11
CATCGTT	210	0.0	16.761406	28
GCGCAAG	270	0.0	16.596258	1
ATCGTTT	215	0.0	16.371605	29
TCTAGAT	225	0.0	16.355068	2
CGTATAT	245	0.0	16.326046	10
ATAGCGT	240	0.0	16.000326	6
AAGACGG	300	0.0	16.000326	5
CAAGACG	300	0.0	16.000326	4
AATAGCG	240	0.0	16.000326	5
TAGTGCT	60	0.0024412712	16.000326	4
>>END_MODULE