FastQCFastQC Report
Thu 2 Feb 2017
SRR4062833_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062833_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345831
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39270.16740336366942035No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT34080.14527900773755653No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32550.13875679876342328No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA31210.13304453730895363No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30690.1308278388340848No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG30280.1290800573442844No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG29630.12630918425069837No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28760.12260047718697553No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC28650.12213156020190713No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG27200.11595038176236906No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA26630.11352053920337823No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG25960.11066440847614341No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT25760.10981183213965541No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG25640.10930028633776262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24770.10559157927403978No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC23860.10171235694301933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT7550.016.7404729
GTATCAA32950.016.6734891
TACCGTC6950.016.1135627
CTCGACG703.698748E-415.99982913
TCTAGCG7000.015.76991528
ATACCGT6800.015.7621866
CATCGTT7850.015.69276228
CTAGCGG6950.015.65350829
CGTCGTA6850.015.64946910
CGAGCCG8950.015.55290715
ACCGTCG6800.015.5279238
CGGCATC8150.015.50936825
CTATTCC7350.015.4536414
CGTTTAT8000.015.19886731
GCATCGT8400.015.04617627
CGCGAAC756.2482915E-414.93317412
TCGTTTA8150.014.91913230
AGGCCCG9800.014.85698410
GGCATCG8750.014.44525226
TAGCGGC7450.014.38818730
Produced by FastQC (version 0.11.5)