##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062833_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345831
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248558826275207	32.0	32.0	32.0	32.0	32.0
2	30.772048796354042	32.0	32.0	32.0	32.0	32.0
3	30.817181629878707	32.0	32.0	32.0	32.0	32.0
4	30.902648571018116	32.0	32.0	32.0	32.0	32.0
5	30.765192377456007	32.0	32.0	32.0	32.0	32.0
6	34.43998139678434	36.0	36.0	36.0	32.0	36.0
7	34.37671383829441	36.0	36.0	36.0	32.0	36.0
8	34.34542471303346	36.0	36.0	36.0	32.0	36.0
9	34.535183480821935	36.0	36.0	36.0	32.0	36.0
10	34.189049850564686	36.0	36.0	36.0	32.0	36.0
11	34.50892157192909	36.0	36.0	36.0	32.0	36.0
12	34.28765541933754	36.0	36.0	36.0	32.0	36.0
13	34.40275492991609	36.0	36.0	36.0	32.0	36.0
14	34.284804404068325	36.0	36.0	36.0	32.0	36.0
15	34.21453378355048	36.0	36.0	36.0	32.0	36.0
16	34.23642666500699	36.0	36.0	36.0	32.0	36.0
17	34.153391697867406	36.0	36.0	36.0	32.0	36.0
18	34.18281794383312	36.0	36.0	36.0	32.0	36.0
19	34.16704315016725	36.0	36.0	36.0	32.0	36.0
20	34.16458986175901	36.0	36.0	36.0	32.0	36.0
21	34.15287674176017	36.0	36.0	36.0	32.0	36.0
22	34.12377106449697	36.0	36.0	36.0	32.0	36.0
23	34.061693702572775	36.0	36.0	36.0	32.0	36.0
24	34.04090149716668	36.0	36.0	36.0	32.0	36.0
25	34.02819171543048	36.0	36.0	36.0	32.0	36.0
26	33.96311243222551	36.0	36.0	36.0	32.0	36.0
27	33.97837226978414	36.0	36.0	36.0	32.0	36.0
28	33.95743470011267	36.0	36.0	36.0	32.0	36.0
29	33.922060881623615	36.0	36.0	36.0	32.0	36.0
30	33.89745595484074	36.0	36.0	36.0	32.0	36.0
31	33.920280275944855	36.0	36.0	36.0	32.0	36.0
32	33.87750438970241	36.0	36.0	36.0	32.0	36.0
33	33.83933497340601	36.0	36.0	36.0	32.0	36.0
34	33.85340930356875	36.0	36.0	36.0	32.0	36.0
35	33.815609052826055	36.0	36.0	36.0	32.0	36.0
36	33.76916921977755	36.0	36.0	36.0	32.0	36.0
37	33.77627714869485	36.0	36.0	36.0	32.0	36.0
38	33.1772996434952	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	1.0
7	3.0
8	6.0
9	11.0
10	12.0
11	26.0
12	13.0
13	14.0
14	151.0
15	455.0
16	588.0
17	742.0
18	904.0
19	1261.0
20	1780.0
21	2861.0
22	4464.0
23	7189.0
24	11135.0
25	16618.0
26	24650.0
27	35394.0
28	49579.0
29	65730.0
30	88936.0
31	119211.0
32	161990.0
33	241941.0
34	525040.0
35	985125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.867551670008474	17.01403559987401	11.318192332873247	25.800220397244267
2	17.108699407357804	19.254281674582067	36.67105265963912	26.965966258421
3	18.84132363392655	23.21782353395098	28.4100729331383	29.53077989898417
4	12.797505620793997	15.135943496206336	35.74083215887088	36.325718724128784
5	15.191785242475255	35.88376295438666	33.345596243883286	15.578855559254794
6	34.86786098707278	35.16937537966387	16.5210959896838	13.44166764357955
7	30.91404747999642	30.10533585127652	20.685429037909824	18.295187630817235
8	28.534316983265136	32.36005645784567	19.29678351417265	19.80884304471654
9	27.541250980998992	14.230187017520077	18.554682088120032	39.6738799133609
10	16.30900830159595	25.846848897653434	30.952539289133753	26.891603511616864
11	37.93101096303042	20.988851104935517	21.949123388125038	19.131014543909032
12	25.14648726834336	23.45429021722959	27.97413383341142	23.425088681015634
13	29.249423041421213	18.82362270724783	25.267097820539767	26.65985643079119
14	23.897641890716606	19.65622559268215	24.50221861453406	31.943913902067184
15	25.470571405546526	26.726451815918477	21.873314438701957	25.929662339833037
16	26.450792411033085	25.427839712683053	23.04333756970075	25.07803030658311
17	24.594006197779784	25.606133994156966	24.64900850872959	25.15085129933366
18	25.758707422091486	24.33060493669267	25.445751801168093	24.464935840047747
19	25.95633691913049	24.710367263901624	25.006107067443768	24.32718874952412
20	26.082630380112708	23.89829215086846	24.383179598064373	25.635897870954455
21	27.512289618485735	23.863410125596896	23.962613810329547	24.661686445587826
22	26.346598774166345	23.667917340426122	24.568811712138412	25.416672173269117
23	24.642308394113904	23.66332480103138	25.23183873096646	26.462528073888258
24	25.163750780755368	24.74400816888618	24.640916484223947	25.45132456613451
25	25.26482294329197	24.15219351634132	24.74619846638257	25.836785073984142
26	25.1379719655974	25.132130754445715	25.13856887768589	24.591328402270996
27	25.99296478084538	24.227765113549594	24.25398411597411	25.52528598963092
28	24.962558847515922	24.27005206590325	25.356730092455027	25.4106589941258
29	24.896603971788796	24.56751874685847	25.248284762104934	25.2875925192478
30	24.83056678384252	24.723906346850193	25.4658827280858	24.97964414122149
31	25.62312049227222	24.513257057399642	24.073046185904897	25.790576264423237
32	25.214181692922118	24.42212721983295	24.095952034214502	26.267739053030432
33	24.936658835085314	24.020274288666887	24.834042524165138	26.20902435208266
34	25.941668944035563	24.119830674602852	24.907324231531888	25.031176149829697
35	26.43684188759546	23.915437579063024	24.972723656424233	24.674996876917284
36	25.027009373766244	24.71236189405845	24.51930989024857	25.741318841926734
37	26.199915330940488	24.487477207353503	24.374030899517095	24.938576562188913
38	25.144425009197757	24.170592676497783	25.07510612056797	25.609876193736486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95.0
1	145.0
2	195.0
3	195.0
4	510.0
5	825.0
6	825.0
7	963.5
8	1102.0
9	1074.5
10	1047.0
11	1047.0
12	1447.0
13	1847.0
14	2736.0
15	3625.0
16	3625.0
17	5507.0
18	7389.0
19	7389.0
20	9426.0
21	11463.0
22	13223.5
23	14984.0
24	14984.0
25	18070.5
26	21157.0
27	21157.0
28	26716.0
29	32275.0
30	39486.0
31	46697.0
32	46697.0
33	61262.0
34	75827.0
35	75827.0
36	84728.5
37	93630.0
38	108178.5
39	122727.0
40	122727.0
41	131476.0
42	140225.0
43	160853.0
44	181481.0
45	181481.0
46	185083.5
47	188686.0
48	188686.0
49	202800.5
50	216915.0
51	220809.0
52	224703.0
53	224703.0
54	215426.5
55	206150.0
56	206150.0
57	200551.5
58	194953.0
59	175177.0
60	155401.0
61	155401.0
62	148042.0
63	140683.0
64	117132.5
65	93582.0
66	93582.0
67	78543.0
68	63504.0
69	63504.0
70	51468.0
71	39432.0
72	31108.0
73	22784.0
74	22784.0
75	17496.5
76	12209.0
77	12209.0
78	11658.0
79	11107.0
80	8784.5
81	6462.0
82	6462.0
83	6336.0
84	6210.0
85	6210.0
86	4199.0
87	2188.0
88	1887.0
89	1586.0
90	1586.0
91	1143.5
92	701.0
93	588.0
94	475.0
95	475.0
96	427.0
97	379.0
98	379.0
99	754.5
100	1130.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1188917701232527
2	0.05311550576320289
3	0.010998234740695301
4	0.00328241889547883
5	1.7051526729760158E-4
6	2.5577290094640237E-4
7	4.2628816824400394E-5
8	4.2628816824400394E-4
9	3.4103053459520315E-4
10	7.246898860148067E-4
11	0.0016198950393272152
12	0.0027282442767616252
13	0.014579055353944936
14	0.009506226151841287
15	0.023190076352473814
16	0.011424522908939305
17	0.020248687991590186
18	0.0055843750039964515
19	0.008014217562987274
20	0.005627003820820852
21	0.006479580157308861
22	0.006905868325552864
23	0.010017771953734092
24	0.014749570621242535
25	0.016881011462462556
26	0.01820250478401897
27	0.00869627863217768
28	0.006394322523660059
29	0.010145658404207294
30	0.0029413883608836273
31	0.0058401479049428546
32	0.006777981875079663
33	0.00852576336488008
34	0.012277099245427314
35	0.016710496195164955
36	0.014877457071715738
37	0.00971937023596329
38	0.006394322523660059
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2345831.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.42376358819332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.32485279291393	42.1093109307215
2	11.737366014951906	12.30633802231867
3	3.3949023114448194	5.339206685405828
4	1.4879135808801318	3.120081192148888
5	0.7985561831310303	2.093166027817557
6	0.5021095148547232	1.5793482301275874
7	0.33004256719809416	1.211145146178331
8	0.24122041694136942	1.011654568830382
9	0.17677452972768234	0.8340467539372265
>10	0.8483084046196351	7.869730490604887
>50	0.06486335888908873	2.41401807478477
>100	0.07197185497950953	8.382642062233039
>500	0.013342288850024664	4.8566068237088995
>1k	0.007776180618112499	6.872704991182371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3927	0.16740336366942035	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3408	0.14527900773755653	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3255	0.13875679876342328	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3121	0.13304453730895363	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3069	0.1308278388340848	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3028	0.1290800573442844	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2963	0.12630918425069837	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2876	0.12260047718697553	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2865	0.12213156020190713	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2720	0.11595038176236906	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2663	0.11352053920337823	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2596	0.11066440847614341	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2576	0.10981183213965541	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2564	0.10930028633776262	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2477	0.10559157927403978	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2386	0.10171235694301933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7051526729760158E-4
2	0.0	0.0	0.0	0.0	1.7051526729760158E-4
3	0.0	0.0	0.0	0.0	1.7051526729760158E-4
4	0.0	0.0	0.0	0.0	1.7051526729760158E-4
5	0.0	0.0	0.0	0.0	1.7051526729760158E-4
6	0.0	0.0	0.0	0.0	1.7051526729760158E-4
7	0.0	0.0	0.0	4.2628816824400394E-5	2.1314408412200197E-4
8	0.0	0.0	0.0	4.2628816824400394E-5	2.1314408412200197E-4
9	0.0	0.0	0.0	4.2628816824400394E-5	2.1314408412200197E-4
10	4.2628816824400394E-5	0.0	0.0	4.2628816824400394E-5	2.1314408412200197E-4
11	4.2628816824400394E-5	0.0	0.0	4.2628816824400394E-5	2.1314408412200197E-4
12	4.2628816824400394E-5	0.0	0.0	4.2628816824400394E-5	5.541746187172051E-4
13	4.2628816824400394E-5	4.2628816824400394E-5	0.0	8.525763364880079E-5	6.394322523660059E-4
14	4.2628816824400394E-5	4.2628816824400394E-5	0.0	8.525763364880079E-5	6.394322523660059E-4
15	4.2628816824400394E-5	4.2628816824400394E-5	0.0	1.7051526729760158E-4	6.394322523660059E-4
16	4.2628816824400394E-5	4.2628816824400394E-5	0.0	2.1314408412200197E-4	7.246898860148067E-4
17	4.2628816824400394E-5	4.2628816824400394E-5	0.0	2.9840171777080276E-4	7.246898860148067E-4
18	4.2628816824400394E-5	4.2628816824400394E-5	0.0	3.4103053459520315E-4	7.673187028392071E-4
19	4.2628816824400394E-5	4.2628816824400394E-5	0.0	3.8365935141960355E-4	7.673187028392071E-4
20	8.525763364880079E-5	4.2628816824400394E-5	0.0	4.6891698506840434E-4	8.525763364880079E-4
21	8.525763364880079E-5	4.2628816824400394E-5	0.0	6.394322523660059E-4	8.525763364880079E-4
22	8.525763364880079E-5	4.2628816824400394E-5	0.0	7.246898860148067E-4	9.378339701368087E-4
23	1.2788645047320118E-4	4.2628816824400394E-5	0.0	9.378339701368087E-4	9.378339701368087E-4
24	1.2788645047320118E-4	4.2628816824400394E-5	0.0	0.0013214933215564121	9.378339701368087E-4
25	1.7051526729760158E-4	4.2628816824400394E-5	0.0	0.0015346374056784142	9.378339701368087E-4
26	1.7051526729760158E-4	4.2628816824400394E-5	0.0	0.001960925573922418	0.0010230916037856095
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	755	0.0	16.74047	29
GTATCAA	3295	0.0	16.673489	1
TACCGTC	695	0.0	16.113562	7
CTCGACG	70	3.698748E-4	15.999829	13
TCTAGCG	700	0.0	15.769915	28
ATACCGT	680	0.0	15.762186	6
CATCGTT	785	0.0	15.692762	28
CTAGCGG	695	0.0	15.653508	29
CGTCGTA	685	0.0	15.649469	10
CGAGCCG	895	0.0	15.552907	15
ACCGTCG	680	0.0	15.527923	8
CGGCATC	815	0.0	15.509368	25
CTATTCC	735	0.0	15.453641	4
CGTTTAT	800	0.0	15.198867	31
GCATCGT	840	0.0	15.046176	27
CGCGAAC	75	6.2482915E-4	14.933174	12
TCGTTTA	815	0.0	14.919132	30
AGGCCCG	980	0.0	14.856984	10
GGCATCG	875	0.0	14.445252	26
TAGCGGC	745	0.0	14.388187	30
>>END_MODULE