FastQCFastQC Report
Thu 2 Feb 2017
SRR4062833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345831
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40220.17145310126773838No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC35630.1518864743453386No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32870.14012092090180409No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG32760.13965200391673568No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA31890.13594329685301285No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC31210.13304453730895363No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC30420.12967686077982601No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG29050.12383671287488314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28920.12328253825616593No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC28880.12311202298886835No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG27750.1182949666877111No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA27420.11688821573250588No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG26450.11275322050053904No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA26320.11219904588182185No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG25000.10657204206100097No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24370.10388642660106376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAT550.001365621817.45377722
TCTAGCG6750.017.06591628
ATCGTTT5650.016.7072329
CTAGCGG6950.016.57481229
GTATCAA32550.016.4181461
CATCGTT5850.016.13604428
TAATACG1001.3149365E-616.0010034
CGTTTAT6000.015.73264131
TAGCGGC7650.015.05816130
AAGACGG7900.014.9889195
TACCGTC7500.014.9352247
ACCGTCG7300.014.9059968
CGTCGTA7350.014.80333210
GGTATCA15950.014.7463761
CTATACT2400.014.6675864
CAAGACG8200.014.6350634
TCGTTTA6600.014.54481430
GCATCGT6600.014.54481427
ACGAACG3750.014.50633915
ATACCGT8000.014.4018236
Produced by FastQC (version 0.11.5)