##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062833_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345831
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197176181915918	32.0	32.0	32.0	32.0	32.0
2	31.236557961762802	32.0	32.0	32.0	32.0	32.0
3	31.336507190841967	32.0	32.0	32.0	32.0	32.0
4	31.428718010802996	32.0	32.0	32.0	32.0	32.0
5	31.36213947211031	32.0	32.0	32.0	32.0	32.0
6	34.87148008530879	36.0	36.0	36.0	36.0	36.0
7	34.888223405692905	36.0	36.0	36.0	36.0	36.0
8	34.81931690731344	36.0	36.0	36.0	32.0	36.0
9	34.95146964977443	36.0	36.0	36.0	32.0	36.0
10	34.775370433761	36.0	36.0	36.0	32.0	36.0
11	34.954806207267275	36.0	36.0	36.0	36.0	36.0
12	34.85305676325362	36.0	36.0	36.0	32.0	36.0
13	34.916706275942296	36.0	36.0	36.0	32.0	36.0
14	34.851120562393454	36.0	36.0	36.0	32.0	36.0
15	34.82288877587516	36.0	36.0	36.0	32.0	36.0
16	34.837420939530595	36.0	36.0	36.0	32.0	36.0
17	34.80102658716677	36.0	36.0	36.0	32.0	36.0
18	34.7968647357802	36.0	36.0	36.0	32.0	36.0
19	34.79601556974906	36.0	36.0	36.0	32.0	36.0
20	34.78896774746348	36.0	36.0	36.0	32.0	36.0
21	34.78905769426698	36.0	36.0	36.0	32.0	36.0
22	34.766499376979844	36.0	36.0	36.0	32.0	36.0
23	34.70140815770616	36.0	36.0	36.0	32.0	36.0
24	34.68398789171087	36.0	36.0	36.0	32.0	36.0
25	34.66086303744814	36.0	36.0	36.0	32.0	36.0
26	34.59381515548222	36.0	36.0	36.0	32.0	36.0
27	34.58728228930388	36.0	36.0	36.0	32.0	36.0
28	34.55224609104407	36.0	36.0	36.0	32.0	36.0
29	34.514412589824246	36.0	36.0	36.0	32.0	36.0
30	34.492467274923044	36.0	36.0	36.0	32.0	36.0
31	34.50198714229627	36.0	36.0	36.0	32.0	36.0
32	34.46208912747764	36.0	36.0	36.0	32.0	36.0
33	34.425690085943955	36.0	36.0	36.0	32.0	36.0
34	34.42444234047551	36.0	36.0	36.0	32.0	36.0
35	34.389849055622506	36.0	36.0	36.0	32.0	36.0
36	34.36558643823873	36.0	36.0	36.0	32.0	36.0
37	34.373565273883756	36.0	36.0	36.0	32.0	36.0
38	33.93292995104933	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	6.0
21	40.0
22	192.0
23	624.0
24	1709.0
25	4169.0
26	8818.0
27	17000.0
28	29290.0
29	45701.0
30	68045.0
31	97703.0
32	140275.0
33	223494.0
34	541749.0
35	1167013.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53048650510652	18.52633921491387	12.179424870439814	26.763749409539788
2	16.029586104028805	20.43646792970167	36.11798974435925	27.415956221910275
3	17.696884387664756	24.106553285381597	28.765925593105386	29.43063673384826
4	12.266447270262551	16.068981980791445	35.6533934684099	36.0111772805361
5	14.57658288256912	36.53033828950167	33.03298489959421	15.86009392833499
6	34.78746097326829	35.614162675183344	16.205280182519775	13.39309616902859
7	30.638979392698246	30.34407172931927	20.611735176499124	18.405213701483365
8	28.3643024738935	32.64216191145275	19.109411981365653	19.88412363328809
9	27.453506440357156	13.98632849551637	18.32169173677558	40.238473327350896
10	16.024803467983855	26.159166954059614	30.968142651486634	26.847886926469894
11	38.20975451918236	20.82308951227404	21.83437686376439	19.13277910477921
12	24.95546346768112	23.56507960731972	27.923116240029632	23.556340684969523
13	29.226043330524092	18.849200005627008	25.235769529663077	26.688987134185826
14	23.71547651983455	19.568459961523228	24.50180767497744	32.21425584366478
15	25.349907985698884	26.870435253008424	21.763588255078904	26.01606850621379
16	26.404172510369467	25.53876453110413	22.90647659890103	25.150586359625375
17	24.573424087242433	25.79303453658853	24.457345819029587	25.17619555713945
18	25.723890595699352	24.342205384786883	25.420501306360094	24.513402713153674
19	25.919684751373822	24.78128219807821	24.889559392812185	24.409473657735788
20	26.074427356446396	23.862759081962853	24.34109703554945	25.721716526041305
21	27.429054961356965	23.959153050306288	23.918826172399534	24.692965815937214
22	26.213653072194887	23.858112540928992	24.57517186873223	25.353062518143894
23	24.568147850693105	23.57519270397719	25.289983144529906	26.566676300799802
24	25.16485629186416	24.72539581922142	24.586340618740223	25.523407270174193
25	25.225740995724326	24.179458869568553	24.827246646176405	25.767553488530716
26	25.190540316451028	25.023307325470018	25.08912627476257	24.69702608331639
27	25.967226951654638	24.216077038830605	24.244297327598336	25.572398681916418
28	24.896081601786317	24.269864282635876	25.343343147907927	25.490710967669877
29	24.86811709794951	24.469495031824543	25.283023372101397	25.379364498124545
30	24.818454526349086	24.653353118788182	25.446163854088383	25.08202850077435
31	25.624906483033094	24.469580289458193	24.12479841898244	25.780714808526273
32	25.28251182629951	24.313345675796764	24.198290499187706	26.205851998716017
33	24.819648133220166	23.971547822498724	24.91624503214426	26.292559012136852
34	25.75854782377759	24.10578596667876	25.021666096151	25.114000113392652
35	26.36328021924853	23.917963399750448	24.909296535001882	24.809459845999136
36	24.98632680700357	24.71968355776695	24.57090898704979	25.723080648179685
37	26.197965667603505	24.518219769454834	24.333594363788354	24.950220199153307
38	25.09029849123829	24.069892502912612	25.07533577653292	25.764473229316177
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	89.0
2	111.0
3	111.0
4	389.0
5	667.0
6	667.0
7	846.5
8	1026.0
9	1062.5
10	1099.0
11	1099.0
12	1457.5
13	1816.0
14	2711.5
15	3607.0
16	3607.0
17	5683.5
18	7760.0
19	7760.0
20	9634.0
21	11508.0
22	13432.0
23	15356.0
24	15356.0
25	18253.5
26	21151.0
27	21151.0
28	26826.0
29	32501.0
30	39809.5
31	47118.0
32	47118.0
33	62079.0
34	77040.0
35	77040.0
36	86171.5
37	95303.0
38	109165.5
39	123028.0
40	123028.0
41	132601.5
42	142175.0
43	162420.5
44	182666.0
45	182666.0
46	185237.5
47	187809.0
48	187809.0
49	202605.5
50	217402.0
51	220742.5
52	224083.0
53	224083.0
54	214993.0
55	205903.0
56	205903.0
57	200272.0
58	194641.0
59	174824.0
60	155007.0
61	155007.0
62	147715.5
63	140424.0
64	116558.5
65	92693.0
66	92693.0
67	78482.0
68	64271.0
69	64271.0
70	51982.0
71	39693.0
72	31173.0
73	22653.0
74	22653.0
75	17208.0
76	11763.0
77	11763.0
78	11406.0
79	11049.0
80	8636.0
81	6223.0
82	6223.0
83	5819.0
84	5415.0
85	5415.0
86	3472.0
87	1529.0
88	1236.5
89	944.0
90	944.0
91	566.0
92	188.0
93	136.5
94	85.0
95	85.0
96	54.0
97	23.0
98	23.0
99	28.5
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00865364981535328
2	0.0
3	0.0
4	4.2628816824400394E-5
5	0.0
6	1.2788645047320118E-4
7	5.115458018928047E-4
8	0.0023445849253420216
9	0.0032397900786544304
10	0.0012788645047320118
11	0.004603912217035242
12	1.7051526729760158E-4
13	8.525763364880079E-5
14	0.0
15	0.0
16	4.2628816824400394E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	4.2628816824400394E-5
22	0.0
23	2.1314408412200197E-4
24	0.0
25	4.2628816824400394E-5
26	8.525763364880079E-5
27	4.2628816824400394E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2345831.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.492549637579984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.05140933476353	39.91507209756202
2	12.475344012085548	12.598238535522318
3	3.6049592108414594	5.460707456845908
4	1.546435404330967	3.1233386565796972
5	0.8512833386279571	2.149173311565847
6	0.5446890086973615	1.6501642085217454
7	0.36302860590787484	1.2831167932565386
8	0.2569844973664725	1.0380641991492126
9	0.19305955741399175	0.8773262357161683
>10	0.9441428575296639	8.345478882851557
>50	0.07007499764251882	2.486834628614485
>100	0.07462666079563768	8.281267536797564
>500	0.015210248246408934	5.25074613298941
>1k	0.008752265750726893	7.540471324027513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4022	0.17145310126773838	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3563	0.1518864743453386	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3287	0.14012092090180409	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3276	0.13965200391673568	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3189	0.13594329685301285	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3121	0.13304453730895363	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3042	0.12967686077982601	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2905	0.12383671287488314	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2892	0.12328253825616593	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2888	0.12311202298886835	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2775	0.1182949666877111	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2742	0.11688821573250588	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2645	0.11275322050053904	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2632	0.11219904588182185	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2500	0.10657204206100097	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2437	0.10388642660106376	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5577290094640237E-4
2	0.0	0.0	0.0	0.0	2.5577290094640237E-4
3	0.0	0.0	0.0	0.0	2.5577290094640237E-4
4	0.0	0.0	0.0	0.0	2.5577290094640237E-4
5	0.0	0.0	0.0	0.0	2.5577290094640237E-4
6	0.0	0.0	0.0	0.0	2.9840171777080276E-4
7	0.0	0.0	0.0	0.0	3.4103053459520315E-4
8	0.0	0.0	0.0	0.0	3.4103053459520315E-4
9	0.0	0.0	0.0	0.0	3.4103053459520315E-4
10	0.0	0.0	0.0	0.0	3.4103053459520315E-4
11	0.0	0.0	0.0	0.0	3.4103053459520315E-4
12	4.2628816824400394E-5	0.0	0.0	4.2628816824400394E-5	6.394322523660059E-4
13	4.2628816824400394E-5	0.0	0.0	8.525763364880079E-5	7.246898860148067E-4
14	4.2628816824400394E-5	0.0	0.0	8.525763364880079E-5	7.246898860148067E-4
15	4.2628816824400394E-5	8.525763364880079E-5	0.0	1.2788645047320118E-4	8.952051533124083E-4
16	4.2628816824400394E-5	1.2788645047320118E-4	0.0	1.7051526729760158E-4	8.952051533124083E-4
17	4.2628816824400394E-5	1.2788645047320118E-4	0.0	2.1314408412200197E-4	9.378339701368087E-4
18	4.2628816824400394E-5	1.2788645047320118E-4	0.0	2.1314408412200197E-4	9.378339701368087E-4
19	4.2628816824400394E-5	1.2788645047320118E-4	0.0	3.4103053459520315E-4	9.378339701368087E-4
20	4.2628816824400394E-5	1.2788645047320118E-4	0.0	3.8365935141960355E-4	0.0011509780542588105
21	4.2628816824400394E-5	1.2788645047320118E-4	0.0	4.6891698506840434E-4	0.0011509780542588105
22	8.525763364880079E-5	1.2788645047320118E-4	0.0	5.541746187172051E-4	0.001193606871083211
23	8.525763364880079E-5	1.2788645047320118E-4	0.0	7.673187028392071E-4	0.001193606871083211
24	8.525763364880079E-5	1.2788645047320118E-4	0.0	0.001193606871083211	0.0012362356879076113
25	8.525763364880079E-5	1.2788645047320118E-4	0.0	0.0014493797720296134	0.0012362356879076113
26	8.525763364880079E-5	1.2788645047320118E-4	0.0	0.0019182967570980176	0.0012788645047320118
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAAT	55	0.0013656218	17.453777	22
TCTAGCG	675	0.0	17.065916	28
ATCGTTT	565	0.0	16.70723	29
CTAGCGG	695	0.0	16.574812	29
GTATCAA	3255	0.0	16.418146	1
CATCGTT	585	0.0	16.136044	28
TAATACG	100	1.3149365E-6	16.001003	4
CGTTTAT	600	0.0	15.732641	31
TAGCGGC	765	0.0	15.058161	30
AAGACGG	790	0.0	14.988919	5
TACCGTC	750	0.0	14.935224	7
ACCGTCG	730	0.0	14.905996	8
CGTCGTA	735	0.0	14.803332	10
GGTATCA	1595	0.0	14.746376	1
CTATACT	240	0.0	14.667586	4
CAAGACG	820	0.0	14.635063	4
TCGTTTA	660	0.0	14.544814	30
GCATCGT	660	0.0	14.544814	27
ACGAACG	375	0.0	14.506339	15
ATACCGT	800	0.0	14.401823	6
>>END_MODULE