FastQCFastQC Report
Thu 2 Feb 2017
SRR4062832_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062832_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560473
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36520.2340316045199116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24820.15905433801161573No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23650.15155661136078613No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20520.13149859049147278No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20320.1302169278161173No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20310.13015284468234953No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20050.1284866832043874No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19890.127461353064103No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18870.12092487341979002No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18530.1187460468716857No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18440.11816929866777573No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17980.11522147451445812No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17380.11137648648839166No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17200.11022299008057172No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16940.1085568286026096No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16130.10336609476741987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC801.253029E-720.019891
ACCGTCG4300.018.6022668
TACCGTC4650.017.8901797
GTATAGA1650.017.4719051
ATGCGCA550.001364648817.45510511
CGGCTAA652.1010097E-417.230779
TATTAGG1051.1829252E-716.7705042
TATACTG1551.0913936E-1116.5124245
CGTCGTA4850.016.49537510
ATACCGT5000.016.3168626
ATCGTTT3550.016.22535129
CATCGTT3700.015.99897428
TAGGACG1501.1459633E-1015.9969234
CCGTCGT4950.015.8383849
TCTAGCG4300.015.62690628
CGTCTTA1451.2205419E-915.44877115
TACTATA1255.108086E-815.367882
CGTTTAT3750.015.35950831
GTCCTAA2300.015.3195691
TAGCGGC4300.015.25532530
Produced by FastQC (version 0.11.5)