##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062832_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1560473
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25289639743847	32.0	32.0	32.0	32.0	32.0
2	30.88790321908806	32.0	32.0	32.0	32.0	32.0
3	30.933276641120994	32.0	32.0	32.0	32.0	32.0
4	30.996382507098808	32.0	32.0	32.0	32.0	32.0
5	30.921708353813234	32.0	32.0	32.0	32.0	32.0
6	34.570609039695015	36.0	36.0	36.0	32.0	36.0
7	34.501687629327776	36.0	36.0	36.0	32.0	36.0
8	34.49115043964234	36.0	36.0	36.0	32.0	36.0
9	34.6151243885668	36.0	36.0	36.0	32.0	36.0
10	34.35137423076208	36.0	36.0	36.0	32.0	36.0
11	34.60171883781392	36.0	36.0	36.0	32.0	36.0
12	34.424575753633675	36.0	36.0	36.0	32.0	36.0
13	34.50677262599225	36.0	36.0	36.0	32.0	36.0
14	34.412646678282805	36.0	36.0	36.0	32.0	36.0
15	34.357471740940085	36.0	36.0	36.0	32.0	36.0
16	34.37211537783736	36.0	36.0	36.0	32.0	36.0
17	34.29395702456883	36.0	36.0	36.0	32.0	36.0
18	34.32630234550678	36.0	36.0	36.0	32.0	36.0
19	34.30826294335115	36.0	36.0	36.0	32.0	36.0
20	34.28417729752453	36.0	36.0	36.0	32.0	36.0
21	34.267307412560164	36.0	36.0	36.0	32.0	36.0
22	34.24855284263169	36.0	36.0	36.0	32.0	36.0
23	34.195079953321844	36.0	36.0	36.0	32.0	36.0
24	34.16998563896972	36.0	36.0	36.0	32.0	36.0
25	34.14900097598613	36.0	36.0	36.0	32.0	36.0
26	34.094207974120664	36.0	36.0	36.0	32.0	36.0
27	34.100889922478636	36.0	36.0	36.0	32.0	36.0
28	34.08654042716535	36.0	36.0	36.0	32.0	36.0
29	34.0486878017114	36.0	36.0	36.0	32.0	36.0
30	34.0198587223233	36.0	36.0	36.0	32.0	36.0
31	34.039104809887775	36.0	36.0	36.0	32.0	36.0
32	33.987285265429136	36.0	36.0	36.0	32.0	36.0
33	33.950674571107605	36.0	36.0	36.0	32.0	36.0
34	33.95783265714947	36.0	36.0	36.0	32.0	36.0
35	33.90183617403185	36.0	36.0	36.0	32.0	36.0
36	33.872403431523644	36.0	36.0	36.0	32.0	36.0
37	33.858706302512125	36.0	36.0	36.0	32.0	36.0
38	33.261148382573744	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	8.0
10	15.0
11	12.0
12	12.0
13	9.0
14	210.0
15	439.0
16	547.0
17	702.0
18	786.0
19	1007.0
20	1403.0
21	1887.0
22	2919.0
23	4432.0
24	6751.0
25	10093.0
26	14776.0
27	21294.0
28	29894.0
29	40280.0
30	54561.0
31	73715.0
32	99721.0
33	148326.0
34	332718.0
35	713946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72119641230288	16.8891227079671	11.250304748951022	26.139376130779002
2	16.818503854969784	19.795149630532002	37.078493917001936	26.30785259749627
3	18.72210472345062	24.228033070563352	28.370249311029934	28.679612894956097
4	12.721974640258493	15.343053755833344	35.79394344877617	36.14102815513199
5	14.813210361993038	36.268705878734124	33.51825601484679	15.399827744426045
6	34.28251378271533	35.26234740965697	16.91177460109749	13.543364206530217
7	30.188667154125703	30.249738380606395	21.273485667486717	18.288108797781184
8	28.389980941590526	32.329317011713165	19.766915780286144	19.513786266410165
9	27.972039175401754	13.986820628926303	18.351790391222124	39.68934980444981
10	16.03966258732184	26.34756479326969	31.339096152872575	26.273676466535896
11	37.69372002037893	20.95723307923752	22.158308420408414	19.190738479975135
12	25.12994857170322	23.534742137558677	28.451223224442057	22.884086066296042
13	29.875345541339016	19.074074192764797	24.98011514946806	26.07046511642812
14	24.14059185371187	19.525635158986823	24.75783782536637	31.575935161934936
15	25.519958309750095	26.616122152945014	22.350691664578097	25.513227872726794
16	26.175744477942537	25.20688488279087	23.5002768719621	25.1170937673045
17	24.447320280541444	25.389103187819444	25.029516846716227	25.13405968492288
18	25.56988195725749	24.04748370094984	26.00221614530428	24.38041819648839
19	25.96751969134734	24.844167579935014	25.091999769279578	24.09631295943807
20	26.126933284114017	23.736271623974677	24.892733293470815	25.24406179844049
21	27.360480296032165	23.97660556367684	23.889703153929418	24.773210986361576
22	26.18213093436747	23.986518314289395	24.491216331835584	25.340134419507553
23	24.42719715953881	23.749126776089366	25.25869859194647	26.56497747242535
24	25.106394433648326	24.875018586979373	24.780673643406878	25.237913335965423
25	25.38500143251875	24.02721721535019	24.893522515541385	25.69425883658967
26	24.960917144346016	24.927330565645416	25.285822425707323	24.825929864301248
27	25.812048087756693	24.5130787777332	24.27889514546431	25.395977989045793
28	24.758150465498268	24.149192724038066	25.441000890172916	25.65165592029075
29	24.75507820961947	24.718033908994073	25.34714611477068	25.179741766615777
30	24.865067860196078	24.703956770446815	25.618073152381353	24.81290221697575
31	25.338397953814713	24.57402649504892	24.283005216080575	25.804570335055793
32	24.903755135889995	24.701299650141024	24.217239414667162	26.17770579930182
33	24.608909599780045	24.186303167881913	25.02337665999922	26.181410572338827
34	25.42173979643176	24.437867205721087	25.15838675915535	24.98200623869181
35	26.239284192040095	24.11781860600758	25.17960895913652	24.4632882428158
36	25.014308888688063	25.007194502822998	24.53963193628587	25.43886467220307
37	25.9872409806269	24.827021331685796	24.480359006042395	24.70537868164491
38	24.9627815734983	24.690925240790243	24.915613666644663	25.430679519066796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	486.0
1	419.0
2	352.0
3	352.0
4	745.0
5	1138.0
6	1138.0
7	1276.5
8	1415.0
9	1351.0
10	1287.0
11	1287.0
12	1650.0
13	2013.0
14	2584.0
15	3155.0
16	3155.0
17	4676.0
18	6197.0
19	6197.0
20	7466.5
21	8736.0
22	9356.5
23	9977.0
24	9977.0
25	11775.0
26	13573.0
27	13573.0
28	17136.5
29	20700.0
30	25931.5
31	31163.0
32	31163.0
33	40347.0
34	49531.0
35	49531.0
36	55598.5
37	61666.0
38	70748.0
39	79830.0
40	79830.0
41	86664.5
42	93499.0
43	106663.5
44	119828.0
45	119828.0
46	126461.5
47	133095.0
48	133095.0
49	140507.0
50	147919.0
51	148234.5
52	148550.0
53	148550.0
54	142527.0
55	136504.0
56	136504.0
57	133942.5
58	131381.0
59	118078.0
60	104775.0
61	104775.0
62	99132.0
63	93489.0
64	76237.5
65	58986.0
66	58986.0
67	49189.5
68	39393.0
69	39393.0
70	31657.0
71	23921.0
72	18720.0
73	13519.0
74	13519.0
75	10070.0
76	6621.0
77	6621.0
78	6500.0
79	6379.0
80	5006.0
81	3633.0
82	3633.0
83	3809.5
84	3986.0
85	3986.0
86	2569.0
87	1152.0
88	1051.0
89	950.0
90	950.0
91	684.5
92	419.0
93	365.0
94	311.0
95	311.0
96	273.5
97	236.0
98	236.0
99	472.0
100	708.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11618272152097472
2	0.05113834074668386
3	0.011086382141824946
4	0.00301190728708539
5	6.408313376777426E-5
6	2.5633253507109703E-4
7	0.0
8	4.485819363744198E-4
9	2.5633253507109703E-4
10	6.408313376777426E-4
11	0.002435159083175422
12	0.0030759904208531646
13	0.015379952104265822
14	0.00993288573400501
15	0.025376920972038607
16	0.011855379747038238
17	0.022236847417417667
18	0.006792812179384071
19	0.009163888128791719
20	0.007049144714455168
21	0.006536479644312974
22	0.008587139924881752
23	0.010445550804147203
24	0.015444035238033598
25	0.01819960999004789
26	0.020891101608294407
27	0.010189218269076107
28	0.006536479644312974
29	0.011470880944431593
30	0.0037809048922986814
31	0.006792812179384071
32	0.008330807389810653
33	0.009996968867772784
34	0.013457458091232593
35	0.017815111187441245
36	0.016277115977014662
37	0.010573717071682752
38	0.0057674820390996836
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1560473.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.05736340163577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71087148347168	41.49537803876171
2	11.944201327630902	12.435672581095645
3	3.5402426274472245	5.52887090960946
4	1.5159455924040754	3.1566452240354788
5	0.8094932230208571	2.1070041440979077
6	0.49977506220149287	1.5610183219258942
7	0.3442655519103488	1.254508985972321
8	0.26039153200672316	1.0844237286706127
9	0.18409607344073933	0.8625200576326915
>10	1.002207296775736	9.442483277600205
>50	0.0869633350487492	3.1564582729594357
>100	0.08474605877090088	9.61853096980671
>500	0.011859413555738533	4.252391743191577
>1k	0.004941422314891056	4.044093744640407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3652	0.2340316045199116	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2482	0.15905433801161573	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2365	0.15155661136078613	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2052	0.13149859049147278	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2032	0.1302169278161173	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2031	0.13015284468234953	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2005	0.1284866832043874	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1989	0.127461353064103	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1887	0.12092487341979002	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1853	0.1187460468716857	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1844	0.11816929866777573	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1798	0.11522147451445812	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1738	0.11137648648839166	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1720	0.11022299008057172	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1694	0.1085568286026096	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1613	0.10336609476741987	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.408313376777426E-5
2	0.0	0.0	0.0	0.0	6.408313376777426E-5
3	0.0	0.0	0.0	0.0	6.408313376777426E-5
4	0.0	0.0	0.0	0.0	6.408313376777426E-5
5	0.0	0.0	0.0	0.0	6.408313376777426E-5
6	0.0	0.0	0.0	0.0	6.408313376777426E-5
7	0.0	0.0	0.0	0.0	6.408313376777426E-5
8	0.0	0.0	0.0	0.0	6.408313376777426E-5
9	0.0	0.0	0.0	0.0	6.408313376777426E-5
10	6.408313376777426E-5	0.0	0.0	0.0	1.9224940130332276E-4
11	6.408313376777426E-5	0.0	0.0	0.0	1.9224940130332276E-4
12	6.408313376777426E-5	0.0	0.0	0.0	3.844988026066455E-4
13	6.408313376777426E-5	0.0	0.0	6.408313376777426E-5	4.485819363744198E-4
14	6.408313376777426E-5	0.0	0.0	6.408313376777426E-5	4.485819363744198E-4
15	6.408313376777426E-5	0.0	0.0	6.408313376777426E-5	5.767482039099683E-4
16	6.408313376777426E-5	0.0	0.0	1.2816626753554852E-4	5.767482039099683E-4
17	6.408313376777426E-5	0.0	0.0	1.9224940130332276E-4	5.767482039099683E-4
18	6.408313376777426E-5	0.0	0.0	2.5633253507109703E-4	5.767482039099683E-4
19	6.408313376777426E-5	0.0	0.0	2.5633253507109703E-4	5.767482039099683E-4
20	6.408313376777426E-5	0.0	0.0	3.844988026066455E-4	6.408313376777426E-4
21	6.408313376777426E-5	0.0	0.0	5.126650701421941E-4	6.408313376777426E-4
22	6.408313376777426E-5	0.0	0.0	7.049144714455168E-4	6.408313376777426E-4
23	6.408313376777426E-5	0.0	0.0	0.001217579541587711	6.408313376777426E-4
24	6.408313376777426E-5	0.0	0.0	0.0019224940130332278	6.408313376777426E-4
25	6.408313376777426E-5	0.0	0.0	0.002178826548104325	6.408313376777426E-4
26	6.408313376777426E-5	0.0	0.0	0.003075990420853164	9.612470065166139E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	80	1.253029E-7	20.01989	1
ACCGTCG	430	0.0	18.602266	8
TACCGTC	465	0.0	17.890179	7
GTATAGA	165	0.0	17.471905	1
ATGCGCA	55	0.0013646488	17.455105	11
CGGCTAA	65	2.1010097E-4	17.23077	9
TATTAGG	105	1.1829252E-7	16.770504	2
TATACTG	155	1.0913936E-11	16.512424	5
CGTCGTA	485	0.0	16.495375	10
ATACCGT	500	0.0	16.316862	6
ATCGTTT	355	0.0	16.225351	29
CATCGTT	370	0.0	15.998974	28
TAGGACG	150	1.1459633E-10	15.996923	4
CCGTCGT	495	0.0	15.838384	9
TCTAGCG	430	0.0	15.626906	28
CGTCTTA	145	1.2205419E-9	15.448771	15
TACTATA	125	5.108086E-8	15.36788	2
CGTTTAT	375	0.0	15.359508	31
GTCCTAA	230	0.0	15.319569	1
TAGCGGC	430	0.0	15.255325	30
>>END_MODULE