FastQCFastQC Report
Thu 2 Feb 2017
SRR4062832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560473
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42140.2700463256974007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31340.20083654122820452No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23940.15341502224005157No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22760.1458532124554542No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22440.14380255217488544No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20560.13175492302654387No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20150.12912751454206514No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20120.1289352651407618No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19260.12342411563673321No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18450.11823338180154351No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18130.11618272152097472No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18050.11567005645083253No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17940.11496514197938702No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17930.11490105884561924No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17160.10996665754550064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16840.10791599726493184No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16250.10413509237263317No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15740.10086685255047667No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15740.10086685255047667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT3500.017.37092629
CATCGTT3600.016.888428
TCTAGCG3900.016.82002428
CTAGCGG4000.016.39952329
GTATTAG3550.016.22541
TAGGACG1753.6379788E-1215.54390054
TACCGTC3750.015.3615247
TATTAGC2500.015.3600452
CGCTTCG4300.015.2553732
TTATACC1052.1845208E-615.2391174
CGTCGTA3950.014.98738710
ATACCGT3850.014.96252256
GGTCGTA1401.2798409E-814.8567129
TCGTTTA4100.014.82883630
ACCTACG650.004159724314.7706957
ACCGTCG3900.014.7706958
TAGCGGC4350.014.71221630
CTATTAT1205.4196244E-714.666711
TCGCGTA1654.838512E-1014.5464329
CGTTTAT4200.014.4757731
Produced by FastQC (version 0.11.5)