FastQCFastQC Report
Thu 2 Feb 2017
SRR4062831_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062831_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774625
Sequences flagged as poor quality0
Sequence length38
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA52440.2954990490948792No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA49130.2768472212439248No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC44190.24901035430020427No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG44030.24810875537085297No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA30960.174459392829471No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA25610.14431217862928788No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA21620.12182855532859055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20870.11760231034725648No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA20530.11568641262238501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7950.022.764651
GTATCAA25100.022.3328441
TACCCGT400.004485086519.9994919
TAGGACC19000.018.9447484
CTAGCGG1105.2023097E-1018.90807529
AGGACCT28100.018.9002365
GGACCTG27000.018.781536
TCTAGCG1159.422365E-1018.08598528
GTCCTAC46700.017.9020731
TGCGAGT450.00885341817.77732518
AGCGCGT450.00885485517.77682332
ATATGCG550.001365275717.454115
TGTAGGA51450.017.2989062
CACGTTT25400.017.25891528
ACGTTTT25350.017.22984529
TACGGCA753.2360018E-517.0662316
AAGGACC11500.016.9715674
GTAGGAC50300.016.9533633
TCACGTT24800.016.90231727
TCCTACA49750.016.892562
Produced by FastQC (version 0.11.5)