##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062831_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1774625 Sequences flagged as poor quality 0 Sequence length 38 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.320321758117913 32.0 32.0 32.0 32.0 32.0 2 30.981765725153203 32.0 32.0 32.0 32.0 32.0 3 31.01161090371205 32.0 32.0 32.0 32.0 32.0 4 31.07884031837712 32.0 32.0 32.0 32.0 32.0 5 31.020692822427275 32.0 32.0 32.0 32.0 32.0 6 34.6815383531732 36.0 36.0 36.0 32.0 36.0 7 34.63237867155033 36.0 36.0 36.0 32.0 36.0 8 34.6137290976967 36.0 36.0 36.0 32.0 36.0 9 34.75388265126435 36.0 36.0 36.0 32.0 36.0 10 34.502368105937876 36.0 36.0 36.0 32.0 36.0 11 34.72305360287385 36.0 36.0 36.0 32.0 36.0 12 34.566214270620556 36.0 36.0 36.0 32.0 36.0 13 34.63463492287103 36.0 36.0 36.0 32.0 36.0 14 34.55706980347961 36.0 36.0 36.0 32.0 36.0 15 34.507173628231314 36.0 36.0 36.0 32.0 36.0 16 34.54475396210467 36.0 36.0 36.0 32.0 36.0 17 34.49908121434106 36.0 36.0 36.0 32.0 36.0 18 34.52671465802634 36.0 36.0 36.0 32.0 36.0 19 34.471061773614146 36.0 36.0 36.0 32.0 36.0 20 34.45783869831654 36.0 36.0 36.0 32.0 36.0 21 34.433471296752835 36.0 36.0 36.0 32.0 36.0 22 34.401793054870744 36.0 36.0 36.0 32.0 36.0 23 34.37563766993026 36.0 36.0 36.0 32.0 36.0 24 34.35795618792703 36.0 36.0 36.0 32.0 36.0 25 34.3331098119321 36.0 36.0 36.0 32.0 36.0 26 34.3107394519969 36.0 36.0 36.0 32.0 36.0 27 34.28203535958301 36.0 36.0 36.0 32.0 36.0 28 34.230393463407765 36.0 36.0 36.0 32.0 36.0 29 34.1840456434458 36.0 36.0 36.0 32.0 36.0 30 34.14280030992463 36.0 36.0 36.0 32.0 36.0 31 34.150903993801506 36.0 36.0 36.0 32.0 36.0 32 34.123328590547295 36.0 36.0 36.0 32.0 36.0 33 34.13118771571459 36.0 36.0 36.0 32.0 36.0 34 34.11521250968514 36.0 36.0 36.0 32.0 36.0 35 34.11668380643798 36.0 36.0 36.0 32.0 36.0 36 34.050750440233855 36.0 36.0 36.0 32.0 36.0 37 34.003821088962454 36.0 36.0 36.0 32.0 36.0 38 33.47916545749102 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 2.0 7 2.0 8 1.0 9 8.0 10 13.0 11 11.0 12 5.0 13 4.0 14 88.0 15 262.0 16 412.0 17 402.0 18 573.0 19 715.0 20 961.0 21 1461.0 22 2191.0 23 3608.0 24 5680.0 25 9063.0 26 14217.0 27 21149.0 28 30642.0 29 42061.0 30 57851.0 31 79139.0 32 109349.0 33 167379.0 34 393850.0 35 833525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.552304371326336 19.600443237302343 12.359866261494211 25.487386129877105 2 13.48030219224131 20.37536656230107 44.25873646024156 21.88559478521606 3 18.575954860132686 25.69944927738791 29.91074145793836 25.813854404541036 4 11.002633333276982 17.227736274461737 40.01364847677477 31.755981915486515 5 11.814804400488892 39.28393724631907 36.383036152508055 12.518222200683976 6 29.951071242817477 39.533117212069506 18.813087414157728 11.702724130955286 7 27.312126187999475 31.87095618109096 22.712273374273508 18.10464425663606 8 24.09655255578803 39.589624589841534 20.29415891523758 16.01966393913285 9 27.10007049400463 14.762323631156885 20.579346890797403 37.55825898404108 10 14.992851986820831 28.714050734270284 34.90805632082154 21.38504095808734 11 34.71727351874653 21.660716952318698 26.342066781434635 17.279942747500137 12 24.62757468792686 24.580803162882116 32.6814622469266 18.11015990226442 13 29.777714406802584 22.479012434737914 25.997698356161912 21.745574802297586 14 20.139376513828886 23.66971846304877 27.266051799011986 28.924853224110358 15 21.45355613821899 35.13657771154553 22.596532125521634 20.813334024713846 16 20.654660296982666 27.926108810237782 30.511252238021196 20.907978654758356 17 20.351398679239647 29.848710548103714 28.94237158601146 20.85751918664518 18 20.77946960902544 26.925625795980928 32.8809156175685 19.413988977425138 19 23.970425388631693 26.051975350733592 27.19472300570021 22.782876254934504 20 24.434655349536243 27.357671324970966 28.328910583436695 19.878762742056097 21 23.34629078413052 26.374367014064603 26.195615054386355 24.08372714741852 22 23.998877435322918 29.876112139252122 26.74944266241685 19.37556776300811 23 20.88484184519945 29.573437387289907 28.859757087210557 20.68196368030008 24 23.577610571739825 26.830783068669756 29.406596404062014 20.185009955528404 25 21.925104756966654 28.314899300868774 29.171666164133807 20.58832977803077 26 19.578248744868446 30.049438769644187 30.058738127819396 20.313574357667967 27 21.19452661263009 28.95805355606187 28.71623582610538 21.13118400520266 28 20.229041187290367 29.322614238828574 27.664595881270966 22.783748692610093 29 21.79221730677111 27.794020682464986 27.050635408154637 23.36312660260926 30 20.213842028061833 28.10370448009981 28.83548285482601 22.846970637012344 31 23.736869124882574 26.718657634461685 27.843569512983375 21.70090372767237 32 21.160583616649227 29.107855021157825 28.162642200345893 21.568919161847056 33 20.017560054145378 29.39990374655815 30.128172616008502 20.454363583287968 34 21.504149827745657 28.80088096838895 29.711943850663687 19.98302535320171 35 20.78415105909087 29.174538084889583 29.65747495423681 20.383835901782735 36 19.836618527650582 28.76432546671363 30.28519901373723 21.113856991898555 37 21.082559108056415 28.29539319523326 27.142987564681576 23.47906013202875 38 20.98514416840141 30.362599054844065 27.284699767035853 21.367557009718674 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 45.0 2 77.0 3 77.0 4 195.0 5 313.0 6 313.0 7 424.5 8 536.0 9 639.5 10 743.0 11 743.0 12 1117.0 13 1491.0 14 2365.5 15 3240.0 16 3240.0 17 4980.5 18 6721.0 19 6721.0 20 9383.0 21 12045.0 22 16668.5 23 21292.0 24 21292.0 25 29680.0 26 38068.0 27 38068.0 28 50645.0 29 63222.0 30 85292.5 31 107363.0 32 107363.0 33 132425.5 34 157488.0 35 157488.0 36 166754.5 37 176021.0 38 180562.0 39 185103.0 40 185103.0 41 174653.5 42 164204.0 43 156614.5 44 149025.0 45 149025.0 46 140043.0 47 131061.0 48 131061.0 49 127648.0 50 124235.0 51 116676.0 52 109117.0 53 109117.0 54 99814.0 55 90511.0 56 90511.0 57 81537.0 58 72563.0 59 60892.5 60 49222.0 61 49222.0 62 44396.0 63 39570.0 64 33046.5 65 26523.0 66 26523.0 67 22686.5 68 18850.0 69 18850.0 70 15029.0 71 11208.0 72 8304.5 73 5401.0 74 5401.0 75 4246.5 76 3092.0 77 3092.0 78 2598.5 79 2105.0 80 1661.0 81 1217.0 82 1217.0 83 1187.0 84 1157.0 85 1157.0 86 826.5 87 496.0 88 408.0 89 320.0 90 320.0 91 238.5 92 157.0 93 121.5 94 86.0 95 86.0 96 87.5 97 89.0 98 89.0 99 384.5 100 680.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12458970204972883 2 0.05928012960484609 3 0.012904134676340072 4 0.0038317954497429033 5 2.2539973233781787E-4 6 2.2539973233781787E-4 7 1.690497992533634E-4 8 3.380995985067268E-4 9 3.380995985067268E-4 10 3.380995985067268E-4 11 0.001690497992533634 12 0.0023103472564626327 13 0.013580333873353527 14 0.008508839895752624 15 0.02237092343452842 16 0.010030288089032894 17 0.019384376981052335 18 0.00478974431217863 19 0.00732549130097908 20 0.004958794111431993 21 0.005578643375360992 22 0.006592942170881172 23 0.009072339226597169 24 0.013185884341762344 25 0.01577798126364725 26 0.01741212932309643 27 0.008339790096499261 28 0.0054659435091920835 29 0.009861238289779532 30 0.0024793970557159964 31 0.004846094245263084 32 0.005860393040783264 33 0.008058040431076989 34 0.012058885680073255 35 0.015383531732056067 36 0.014087483271113616 37 0.009185039092766079 38 0.00580404310769881 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 1774625.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.35073821943857 #Duplication Level Percentage of deduplicated Percentage of total 1 63.32479536202128 24.91877445090417 2 15.853401874564637 12.476861341070995 3 7.442965905515204 8.786586087724062 4 4.163274400913103 6.553116843440858 5 2.5517089021838713 5.020581451102426 6 1.6541663793659147 3.905560089949477 7 1.1130654194296958 3.065996215876129 8 0.7926080995308232 2.4951771068195305 9 0.5818898204977261 2.0608014599065907 >10 2.3030964563913683 14.911105518614749 >50 0.11605437514694132 3.1929273807052474 >100 0.08974236964390045 6.995932971084954 >500 0.009779164848900432 2.740125937684146 >1k 0.0033076586988927937 2.57969105471565 >5k 1.4381124777794753E-4 0.29676209040098434 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA 5244 0.2954990490948792 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA 4913 0.2768472212439248 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC 4419 0.24901035430020427 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG 4403 0.24810875537085297 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA 3096 0.174459392829471 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA 2561 0.14431217862928788 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA 2162 0.12182855532859055 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 2087 0.11760231034725648 No Hit ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA 2053 0.11568641262238501 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 1.1269986616890892E-4 0.0 21 0.0 0.0 0.0 1.1269986616890892E-4 0.0 22 0.0 0.0 0.0 3.380995985067268E-4 0.0 23 0.0 0.0 0.0 4.507994646756357E-4 0.0 24 0.0 0.0 0.0 5.634993308445447E-4 0.0 25 0.0 0.0 0.0 8.452489962668169E-4 0.0 26 0.0 0.0 0.0 0.0010142987955201804 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 795 0.0 22.76465 1 GTATCAA 2510 0.0 22.332844 1 TACCCGT 40 0.0044850865 19.99949 19 TAGGACC 1900 0.0 18.944748 4 CTAGCGG 110 5.2023097E-10 18.908075 29 AGGACCT 2810 0.0 18.900236 5 GGACCTG 2700 0.0 18.78153 6 TCTAGCG 115 9.422365E-10 18.085985 28 GTCCTAC 4670 0.0 17.902073 1 TGCGAGT 45 0.008853418 17.777325 18 AGCGCGT 45 0.008854855 17.776823 32 ATATGCG 55 0.0013652757 17.4541 15 TGTAGGA 5145 0.0 17.298906 2 CACGTTT 2540 0.0 17.258915 28 ACGTTTT 2535 0.0 17.229845 29 TACGGCA 75 3.2360018E-5 17.06623 16 AAGGACC 1150 0.0 16.971567 4 GTAGGAC 5030 0.0 16.953363 3 TCACGTT 2480 0.0 16.902317 27 TCCTACA 4975 0.0 16.89256 2 >>END_MODULE