FastQCFastQC Report
Thu 2 Feb 2017
SRR4062831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774625
Sequences flagged as poor quality0
Sequence length38
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA51230.28868070719166017No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA47060.2651827850954427No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG45640.2571810945974502No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC42510.23954356554201592No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA27790.15659646404169897No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA25270.14239628090441644No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA24190.13631048813129534No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA23910.13473269000493063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT19480.1097696696485173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG350.002064178222.8624765
GTATCAA24350.021.9467851
GGTATCA7750.020.0259651
TAGGACC18050.019.5929434
TCCTCGC9350.017.96653729
GGACCTG28450.017.7761786
AGGACCT29100.017.433625
GCACCGT854.974607E-616.9456086
CCTCGCC9950.016.72233830
ATTTAGA15750.016.4572731
GTCCTAC42400.016.3020151
TTCCTCG10750.016.07318728
TCTCGTA600.00244293815.9996772
GACCTGG31050.015.9268697
TGTAGGA53550.015.7457122
AAATGTC17050.015.6756537
GTAGGAC50700.015.6231863
AATGTCC17150.015.584258
CTAGGAC3700.015.5694463
ATGTCCA17000.015.53259859
Produced by FastQC (version 0.11.5)