##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1774625
Sequences flagged as poor quality	0
Sequence length	38
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26540705782912	32.0	32.0	32.0	32.0	32.0
2	31.333167288863844	32.0	32.0	32.0	32.0	32.0
3	31.395841374938367	32.0	32.0	32.0	32.0	32.0
4	31.480423751496794	32.0	32.0	32.0	32.0	32.0
5	31.436574487567796	32.0	32.0	32.0	32.0	32.0
6	34.94588659575967	36.0	36.0	36.0	36.0	36.0
7	34.97830020426851	36.0	36.0	36.0	36.0	36.0
8	34.894935549764035	36.0	36.0	36.0	36.0	36.0
9	35.01573008382053	36.0	36.0	36.0	36.0	36.0
10	34.89117024723533	36.0	36.0	36.0	32.0	36.0
11	35.034195956892304	36.0	36.0	36.0	36.0	36.0
12	34.96206635204621	36.0	36.0	36.0	36.0	36.0
13	35.007704162851304	36.0	36.0	36.0	36.0	36.0
14	34.95969514686201	36.0	36.0	36.0	36.0	36.0
15	34.930549834472075	36.0	36.0	36.0	36.0	36.0
16	34.95743776854265	36.0	36.0	36.0	36.0	36.0
17	34.94891033316898	36.0	36.0	36.0	36.0	36.0
18	34.94489596393604	36.0	36.0	36.0	36.0	36.0
19	34.92089990843136	36.0	36.0	36.0	36.0	36.0
20	34.90928844121998	36.0	36.0	36.0	36.0	36.0
21	34.89484313587378	36.0	36.0	36.0	32.0	36.0
22	34.87503895189124	36.0	36.0	36.0	32.0	36.0
23	34.836079735155316	36.0	36.0	36.0	32.0	36.0
24	34.82173529618934	36.0	36.0	36.0	32.0	36.0
25	34.80585813904346	36.0	36.0	36.0	32.0	36.0
26	34.74043248573642	36.0	36.0	36.0	32.0	36.0
27	34.69815087694583	36.0	36.0	36.0	32.0	36.0
28	34.662229485102486	36.0	36.0	36.0	32.0	36.0
29	34.63601267873494	36.0	36.0	36.0	32.0	36.0
30	34.59413002747059	36.0	36.0	36.0	32.0	36.0
31	34.58662139888709	36.0	36.0	36.0	32.0	36.0
32	34.572842149749945	36.0	36.0	36.0	32.0	36.0
33	34.57638768753962	36.0	36.0	36.0	32.0	36.0
34	34.5594573501444	36.0	36.0	36.0	32.0	36.0
35	34.549505951961685	36.0	36.0	36.0	32.0	36.0
36	34.50339198422202	36.0	36.0	36.0	32.0	36.0
37	34.45367387476227	36.0	36.0	36.0	32.0	36.0
38	34.098077622032825	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	5.0
21	19.0
22	85.0
23	309.0
24	925.0
25	2478.0
26	5436.0
27	10511.0
28	19014.0
29	30924.0
30	47294.0
31	68573.0
32	98840.0
33	156079.0
34	393660.0
35	940469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.78259448444382	20.996668304712614	12.971858671199094	26.248878539644473
2	12.822941648484413	21.471308851903277	43.204081540653114	22.501667958959192
3	17.734169190674088	26.53811368599	30.26943720504332	25.458279918292597
4	10.607774158229663	18.201105248833134	39.928086134350785	31.263034458586414
5	11.377001550751032	40.031184070541144	35.84652298176966	12.74529139693817
6	30.24281777030953	39.62537395266488	18.433605334766877	11.698202942258721
7	27.15785511385248	32.09862838318717	22.81163132860481	17.93188517435554
8	24.036290379386614	39.81088429623995	20.074102249834468	16.078723074538974
9	26.67778313752716	14.676559161114067	20.480190967369648	38.16546673398913
10	14.831740779538977	29.170979813626975	34.57903216081478	21.418247246019273
11	34.525586645320274	21.696170103897405	26.43683796185939	17.341405288922925
12	24.526883562415758	24.78451676306311	32.450231486699096	18.23836818782204
13	29.668402623206518	22.472278603556138	26.03236069427743	21.826958078959912
14	20.06412622385011	23.872930900894556	27.20270479678805	28.860238078467283
15	21.493949425935057	35.16669718954709	22.401183348594774	20.938170035923083
16	20.734014228358102	28.174853842361063	30.050010565612457	21.04112136366838
17	20.41276325984363	30.235739945058814	28.44088187645277	20.910614918644786
18	20.83752905543425	27.133901528491933	32.57352961893358	19.45503979714024
19	24.079960555046842	26.089061069239982	27.3004155807565	22.530562794956683
20	24.631175600478976	27.62065225047545	27.895245474395995	19.852926674649574
21	23.279436409556514	26.450308854505355	26.255732206633297	24.014522529304834
22	24.13485898984799	29.74230034080459	26.67077645743549	19.45206421191193
23	20.8027399637782	29.867769023487817	28.537908354567904	20.791582658166078
24	23.712277241670776	26.988349651334786	28.90830457138832	20.391068535606113
25	21.969092061703176	28.649038529266747	28.753285905472985	20.628583503557092
26	19.678365670699822	30.31290008475035	29.60598977586238	20.40274446868745
27	21.339631008952324	29.150923886369107	28.409076181250892	21.10036892342768
28	20.3418186940903	29.58630696626048	27.551341832781574	22.52053250686765
29	21.7424526308375	27.721462280763543	27.035289145594138	23.500795942804817
30	20.259096992322323	28.131069944354444	28.702514615763892	22.907318447559344
31	23.537310699443545	26.913685989997887	27.82835810382475	21.720645206733817
32	21.20064802423047	29.181207297316337	27.998985701204482	21.619158977248716
33	20.04378389800662	29.5673733887441	29.84157216313306	20.547270550116224
34	21.66474607311404	28.926618299640772	29.326984574205817	20.081651053039373
35	20.832570261322815	29.35662463900824	29.266633795872366	20.544171303796578
36	19.91029090652955	28.866380221173486	29.89448475029936	21.328844121997605
37	21.241670775515953	28.350496583785308	26.89238571529196	23.515446925406778
38	21.193064667819588	30.170408024690314	27.189831305015204	21.44669600247489
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	19.5
2	35.0
3	35.0
4	134.0
5	233.0
6	233.0
7	319.5
8	406.0
9	522.5
10	639.0
11	639.0
12	996.0
13	1353.0
14	2302.5
15	3252.0
16	3252.0
17	4916.5
18	6581.0
19	6581.0
20	9195.0
21	11809.0
22	16518.0
23	21227.0
24	21227.0
25	29535.5
26	37844.0
27	37844.0
28	50046.0
29	62248.0
30	84302.5
31	106357.0
32	106357.0
33	131902.0
34	157447.0
35	157447.0
36	166576.5
37	175706.0
38	181862.0
39	188018.0
40	188018.0
41	177171.5
42	166325.0
43	158324.5
44	150324.0
45	150324.0
46	141348.5
47	132373.0
48	132373.0
49	128134.5
50	123896.0
51	116398.5
52	108901.0
53	108901.0
54	99751.5
55	90602.0
56	90602.0
57	81434.0
58	72266.0
59	60408.5
60	48551.0
61	48551.0
62	43908.0
63	39265.0
64	32849.0
65	26433.0
66	26433.0
67	22629.0
68	18825.0
69	18825.0
70	14934.5
71	11044.0
72	8050.5
73	5057.0
74	5057.0
75	3987.0
76	2917.0
77	2917.0
78	2434.0
79	1951.0
80	1557.0
81	1163.0
82	1163.0
83	1038.0
84	913.0
85	913.0
86	655.5
87	398.0
88	296.0
89	194.0
90	194.0
91	118.5
92	43.0
93	29.0
94	15.0
95	15.0
96	10.0
97	5.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008621539761921534
2	5.634993308445447E-5
3	0.0
4	1.1269986616890894E-4
5	5.634993308445447E-5
6	1.1269986616890894E-4
7	2.2539973233781787E-4
8	0.002817496654222723
9	0.004564344579840811
10	6.761991970134536E-4
11	0.004846094245263084
12	5.071493977600901E-4
13	1.690497992533634E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.690497992533634E-4
22	5.634993308445447E-5
23	1.690497992533634E-4
24	0.0
25	0.0
26	5.634993308445447E-5
27	1.1269986616890894E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.1269986616890894E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1774625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.278387372441074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.34993465656435	22.497482420289128
2	16.96388820334262	12.647727915739793
3	7.987305873713975	8.932616472674503
4	4.539959509986822	6.769694770739461
5	2.86424308107079	5.338718155249554
6	1.8237846183373085	4.079264969176667
7	1.2046194106151267	3.143438831769064
8	0.8575753564624379	2.55752210714128
9	0.623025978044409	2.0902863377370107
>10	2.547975351597129	15.550182330175552
>50	0.12838672116228222	3.3468369150144306
>100	0.09412099736233544	7.006149217530135
>500	0.01047436680087275	2.7219546229476808
>1k	0.004554072522118587	3.0282169601491953
>5k	1.5180241740395293E-4	0.2899079736665482
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	5123	0.28868070719166017	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	4706	0.2651827850954427	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	4564	0.2571810945974502	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	4251	0.23954356554201592	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	2779	0.15659646404169897	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2527	0.14239628090441644	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2419	0.13631048813129534	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	2391	0.13473269000493063	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1948	0.1097696696485173	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	5.634993308445446E-5	0.0
21	0.0	0.0	0.0	1.1269986616890892E-4	0.0
22	0.0	0.0	0.0	3.380995985067268E-4	0.0
23	0.0	0.0	0.0	3.9444953159118123E-4	0.0
24	0.0	0.0	0.0	5.071493977600902E-4	0.0
25	0.0	0.0	0.0	6.761991970134536E-4	0.0
26	0.0	0.0	0.0	9.015989293512714E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	35	0.0020641782	22.862476	5
GTATCAA	2435	0.0	21.946785	1
GGTATCA	775	0.0	20.025965	1
TAGGACC	1805	0.0	19.592943	4
TCCTCGC	935	0.0	17.966537	29
GGACCTG	2845	0.0	17.776178	6
AGGACCT	2910	0.0	17.43362	5
GCACCGT	85	4.974607E-6	16.945608	6
CCTCGCC	995	0.0	16.722338	30
ATTTAGA	1575	0.0	16.457273	1
GTCCTAC	4240	0.0	16.302015	1
TTCCTCG	1075	0.0	16.073187	28
TCTCGTA	60	0.002442938	15.999677	2
GACCTGG	3105	0.0	15.926869	7
TGTAGGA	5355	0.0	15.745712	2
AAATGTC	1705	0.0	15.675653	7
GTAGGAC	5070	0.0	15.623186	3
AATGTCC	1715	0.0	15.58425	8
CTAGGAC	370	0.0	15.569446	3
ATGTCCA	1700	0.0	15.5325985	9
>>END_MODULE