FastQCFastQC Report
Thu 2 Feb 2017
SRR4062830_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062830_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341376
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT52760.39332744882866544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38530.2872423541199485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20950.15618290471873658No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19530.14559676034161936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17320.12912114127582422No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16850.12561727658762345No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15870.11831134596116226No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15670.11682033971086407No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15540.11585118564817022No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15240.11361467627272294No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15090.11249642158499928No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14940.11137816689727564No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14680.10943985877188797No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA14640.10914165752182833No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14620.10899255689679851No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13980.10422133689584427No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13730.10235757908297152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGC252.8959816E-431.9987349
CGAATAT308.419548E-426.6656112
ACGATTA453.537249E-421.33328432
ACGAACG1401.8189894E-1218.2849915
CCGATAA1505.456968E-1217.065999
AGGCGTA854.9870596E-616.94050612
CGATAAC1551.0913936E-1116.51547410
TTCCGAT1750.016.456497
GATAACG1703.6379788E-1215.99936711
CGAACGA1601.8189894E-1115.99936516
TCTAGCG3300.015.51511628
TTAAGGC1052.183846E-615.2391973
GGTTCGT951.433284E-515.15729318
GTCCTAT4650.015.1550061
TCCGATA1704.7293724E-1115.0582288
AACGAAC1704.7293724E-1115.05822814
TAGCGGC3300.015.03026930
CTAATAG756.241891E-414.9344133
CATCGTT2900.014.89651728
CTAGCGG3400.014.58820129
Produced by FastQC (version 0.11.5)