##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062830_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1341376
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19164350636958	32.0	32.0	32.0	32.0	32.0
2	30.86778129323918	32.0	32.0	32.0	32.0	32.0
3	30.92631819862589	32.0	32.0	32.0	32.0	32.0
4	30.9772517176392	32.0	32.0	32.0	32.0	32.0
5	30.939724581325446	32.0	32.0	32.0	32.0	32.0
6	34.573370926570924	36.0	36.0	36.0	32.0	36.0
7	34.483809163128015	36.0	36.0	36.0	32.0	36.0
8	34.46352849611146	36.0	36.0	36.0	32.0	36.0
9	34.55886418125865	36.0	36.0	36.0	32.0	36.0
10	34.330878143041176	36.0	36.0	36.0	32.0	36.0
11	34.58530344959206	36.0	36.0	36.0	32.0	36.0
12	34.418757305930626	36.0	36.0	36.0	32.0	36.0
13	34.48810847965075	36.0	36.0	36.0	32.0	36.0
14	34.40167782933346	36.0	36.0	36.0	32.0	36.0
15	34.36137518488478	36.0	36.0	36.0	32.0	36.0
16	34.36944376520827	36.0	36.0	36.0	32.0	36.0
17	34.30599772174245	36.0	36.0	36.0	32.0	36.0
18	34.33069400376927	36.0	36.0	36.0	32.0	36.0
19	34.313970877665916	36.0	36.0	36.0	32.0	36.0
20	34.283990469488046	36.0	36.0	36.0	32.0	36.0
21	34.25382890405076	36.0	36.0	36.0	32.0	36.0
22	34.24269779688917	36.0	36.0	36.0	32.0	36.0
23	34.1973890989551	36.0	36.0	36.0	32.0	36.0
24	34.179192858676466	36.0	36.0	36.0	32.0	36.0
25	34.16458621594542	36.0	36.0	36.0	32.0	36.0
26	34.13135317763252	36.0	36.0	36.0	32.0	36.0
27	34.12412701584045	36.0	36.0	36.0	32.0	36.0
28	34.108541527506084	36.0	36.0	36.0	32.0	36.0
29	34.082290125960206	36.0	36.0	36.0	32.0	36.0
30	34.03859767880147	36.0	36.0	36.0	32.0	36.0
31	34.052179254735435	36.0	36.0	36.0	32.0	36.0
32	34.00459528126342	36.0	36.0	36.0	32.0	36.0
33	33.958423290710435	36.0	36.0	36.0	32.0	36.0
34	33.95928732883248	36.0	36.0	36.0	32.0	36.0
35	33.91444307934539	36.0	36.0	36.0	32.0	36.0
36	33.860450015506466	36.0	36.0	36.0	32.0	36.0
37	33.85456799584904	36.0	36.0	36.0	32.0	36.0
38	33.26777503101293	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	2.0
9	3.0
10	9.0
11	9.0
12	10.0
13	8.0
14	268.0
15	457.0
16	623.0
17	690.0
18	868.0
19	1110.0
20	1446.0
21	1938.0
22	2903.0
23	4287.0
24	6009.0
25	8797.0
26	12752.0
27	18252.0
28	25463.0
29	34195.0
30	46352.0
31	61304.0
32	83426.0
33	124240.0
34	278322.0
35	627626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25153967705409	17.666632580171065	11.772306190281766	25.30952155249308
2	16.04461802861951	20.51432765637494	38.6144810177947	24.826573297210853
3	18.57851707979019	25.133443433330477	29.079596333148178	27.20844315373116
4	12.102133534922487	16.157251469051147	36.90758956328811	34.83302543273826
5	13.96913011453944	37.26490488842767	33.833791073617164	14.932173923415725
6	32.39536265507454	36.59069713002592	17.761007208296586	13.252933006602946
7	29.011999618302397	31.39514945846653	21.527073691492916	18.065777231738156
8	27.652462003314533	33.13463056717513	20.220416932875192	18.99249049663515
9	27.785270123284494	14.304863165914822	19.067385633632504	38.84248107716818
10	15.682446103449768	27.044594838396087	32.058811688979745	25.214147369174412
11	36.73870143579979	21.593060089775694	22.895038778299064	18.77319969612545
12	24.50629483865725	24.339520940673374	29.506156916569065	21.64802730410031
13	29.589288683461568	19.989173559326584	25.59014167575705	24.8313960814548
14	23.404352085604238	20.56780208195091	25.96160871788984	30.066237114555015
15	24.735424484122486	27.897728289595353	23.372651780441096	23.99419544584106
16	24.71020765438008	26.202506824016066	25.07181899451171	24.015466527092144
17	23.167786295858008	26.275530938378267	26.301256684033408	24.25542608173032
18	24.068071071136746	25.19115726188843	27.694392919129445	23.04637874784538
19	24.93452096484747	25.49115800037129	26.52219651688065	23.052124517900584
20	25.266664330612326	24.638240861616364	26.281878754306483	23.813216053464828
21	26.66140803571495	24.631641102910862	25.104992443824475	23.601958417549714
22	25.07196532244416	25.01865767618923	25.86375162066428	24.045625380702333
23	23.448401840101994	24.910567165959606	26.435510687950615	25.20552030598779
24	24.058877214121466	25.807209260015927	26.210739327927087	23.923174197935523
25	24.264522214393033	25.119917085155485	26.24560540139509	24.369955299056397
26	23.459675176366808	26.212197924534912	26.962031829166722	23.366095069931557
27	24.561098738711067	25.580374084713704	25.954354417940916	23.904172758634314
28	23.465351923244672	25.664783668990033	26.701538282607707	24.168326125157588
29	23.444339647260133	25.667696827130627	26.658813091115146	24.229150434494095
30	23.578375275378637	25.986945841270074	26.78160191152844	23.65307697182285
31	24.20136942181293	25.734737149741743	25.58808793882931	24.47580548961601
32	23.533955277015444	26.091365032927527	25.655512387802986	24.719167302254043
33	23.436149820653313	25.497201505751693	26.39576158782396	24.670887085771035
34	24.007119008528196	25.82407668368131	26.58630567597029	23.582498631820208
35	24.889049614507062	25.636771701686623	26.268995034075488	23.205183649730827
36	23.494477177253756	26.505746509370233	25.93206488105091	24.067711432325105
37	24.41472070621216	26.35590945692046	25.653425188631417	23.57594464823596
38	23.659256133465647	26.138065696505375	26.128969968440806	24.073708201588172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1237.0
1	863.5
2	490.0
3	490.0
4	1131.5
5	1773.0
6	1773.0
7	1996.0
8	2219.0
9	2121.5
10	2024.0
11	2024.0
12	2582.0
13	3140.0
14	3886.0
15	4632.0
16	4632.0
17	6651.5
18	8671.0
19	8671.0
20	10170.5
21	11670.0
22	12390.0
23	13110.0
24	13110.0
25	15544.5
26	17979.0
27	17979.0
28	23001.0
29	28023.0
30	34152.0
31	40281.0
32	40281.0
33	48425.5
34	56570.0
35	56570.0
36	61436.5
37	66303.0
38	74855.5
39	83408.0
40	83408.0
41	86476.0
42	89544.0
43	99004.5
44	108465.0
45	108465.0
46	111853.5
47	115242.0
48	115242.0
49	118505.5
50	121769.0
51	119387.0
52	117005.0
53	117005.0
54	109048.5
55	101092.0
56	101092.0
57	96752.5
58	92413.0
59	81755.0
60	71097.0
61	71097.0
62	67838.0
63	64579.0
64	53301.0
65	42023.0
66	42023.0
67	35317.5
68	28612.0
69	28612.0
70	23351.5
71	18091.0
72	14121.0
73	10151.0
74	10151.0
75	7778.0
76	5405.0
77	5405.0
78	5312.5
79	5220.0
80	4039.0
81	2858.0
82	2858.0
83	2991.0
84	3124.0
85	3124.0
86	2062.5
87	1001.0
88	884.0
89	767.0
90	767.0
91	555.0
92	343.0
93	293.0
94	243.0
95	243.0
96	226.0
97	209.0
98	209.0
99	401.0
100	593.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12278436471205686
2	0.05606183501121237
3	0.012748103440049622
4	0.002534710625506942
5	2.9820125005964026E-4
6	3.727515625745503E-4
7	0.0
8	6.709528126341906E-4
9	5.218521876043705E-4
10	7.455031251491006E-4
11	0.002460160312992032
12	0.002907462188081492
13	0.016028317190705663
14	0.009989741876997948
15	0.026838112505367624
16	0.01170439906484088
17	0.02288694594207739
18	0.0053676225010735245
19	0.007976883439095377
20	0.006187675938737536
21	0.006634977813826995
22	0.007827782814065556
23	0.010884345627176868
24	0.015804666253160935
25	0.017966625316093325
26	0.020799537191659907
27	0.009616990314423398
28	0.0055912734386182545
29	0.012002600314900521
30	0.0030565628131113123
31	0.005964025001192805
32	0.007231380313946275
33	0.008871487189274297
34	0.01326995562765399
35	0.017593873753518775
36	0.015953766878190754
37	0.010138842502027769
38	0.006485877188797176
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1341376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.62427970660237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76207545534093	43.02321639945065
2	13.397885158120925	14.63699652708268
3	3.926098682628857	6.433809377869252
4	1.4848312442906197	3.24431348820933
5	0.6701405714800761	1.830297300963502
6	0.3706131013600392	1.2146684226973297
7	0.24842111675469353	0.9498877196644433
8	0.1750153581178821	0.76480702998259
9	0.12284540540851525	0.603930760713513
>10	0.663556012232179	6.750476862936488
>50	0.07718605960653606	2.9605551529595333
>100	0.08654288501193125	10.060181216208036
>500	0.010543973359361336	3.8806034501347035
>1k	0.004108041568582338	3.2516135809715823
>5k	1.369347189527446E-4	0.39464271015633573
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5276	0.39332744882866544	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3853	0.2872423541199485	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2095	0.15618290471873658	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1953	0.14559676034161936	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1732	0.12912114127582422	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1685	0.12561727658762345	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1587	0.11831134596116226	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1567	0.11682033971086407	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1554	0.11585118564817022	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1524	0.11361467627272294	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1509	0.11249642158499928	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1494	0.11137816689727564	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1468	0.10943985877188797	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1464	0.10914165752182833	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1462	0.10899255689679851	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1398	0.10422133689584427	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1373	0.10235757908297152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.455031251491006E-5	0.0
6	0.0	0.0	0.0	7.455031251491006E-5	0.0
7	0.0	0.0	0.0	7.455031251491006E-5	0.0
8	0.0	0.0	0.0	7.455031251491006E-5	0.0
9	0.0	0.0	0.0	7.455031251491006E-5	0.0
10	0.0	0.0	0.0	7.455031251491006E-5	0.0
11	0.0	0.0	0.0	7.455031251491006E-5	0.0
12	0.0	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5
13	0.0	0.0	0.0	7.455031251491006E-5	1.4910062502982013E-4
14	0.0	0.0	0.0	7.455031251491006E-5	1.4910062502982013E-4
15	0.0	0.0	0.0	1.4910062502982013E-4	2.2365093754473018E-4
16	0.0	0.0	0.0	1.4910062502982013E-4	2.2365093754473018E-4
17	0.0	0.0	0.0	1.4910062502982013E-4	2.2365093754473018E-4
18	0.0	0.0	0.0	2.2365093754473018E-4	2.2365093754473018E-4
19	0.0	0.0	0.0	2.2365093754473018E-4	2.2365093754473018E-4
20	0.0	0.0	0.0	2.2365093754473018E-4	2.2365093754473018E-4
21	0.0	0.0	0.0	5.218521876043705E-4	2.2365093754473018E-4
22	0.0	0.0	0.0	0.001043704375208741	2.2365093754473018E-4
23	0.0	0.0	0.0	0.002311059687962212	2.2365093754473018E-4
24	0.0	0.0	7.455031251491006E-5	0.003951166563290233	2.2365093754473018E-4
25	0.0	0.0	7.455031251491006E-5	0.004845770313469154	2.2365093754473018E-4
26	0.0	0.0	7.455031251491006E-5	0.006560427501312085	2.2365093754473018E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGC	25	2.8959816E-4	31.998734	9
CGAATAT	30	8.419548E-4	26.66561	12
ACGATTA	45	3.537249E-4	21.333284	32
ACGAACG	140	1.8189894E-12	18.28499	15
CCGATAA	150	5.456968E-12	17.06599	9
AGGCGTA	85	4.9870596E-6	16.940506	12
CGATAAC	155	1.0913936E-11	16.515474	10
TTCCGAT	175	0.0	16.45649	7
GATAACG	170	3.6379788E-12	15.999367	11
CGAACGA	160	1.8189894E-11	15.999365	16
TCTAGCG	330	0.0	15.515116	28
TTAAGGC	105	2.183846E-6	15.239197	3
GGTTCGT	95	1.433284E-5	15.157293	18
GTCCTAT	465	0.0	15.155006	1
TCCGATA	170	4.7293724E-11	15.058228	8
AACGAAC	170	4.7293724E-11	15.058228	14
TAGCGGC	330	0.0	15.030269	30
CTAATAG	75	6.241891E-4	14.934413	3
CATCGTT	290	0.0	14.896517	28
CTAGCGG	340	0.0	14.588201	29
>>END_MODULE