FastQCFastQC Report
Thu 2 Feb 2017
SRR4062830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341376
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT64610.4816695691588339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT49160.3664893363232979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT28590.2131393434801279No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21550.1606559234696312No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19890.14828057159215613No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17960.13389236127677848No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17390.12964299346342859No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16470.12278436471205686No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15660.11674578939834916No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15610.11637303783577461No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15530.11577663533565531No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15180.11316737439763347No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15140.11286917314757383No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14840.11063266377212652No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14130.10533959158356791No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14060.10481773939596355No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13800.10287943127057589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG308.4061513E-426.6728825
TTACACG350.00206526422.8599134
GTAATCG350.002067503322.85565231
TCTAGCG3600.018.6654528
TCGTTTA3700.018.16097630
ATCGTTT3700.018.16097629
CTAGCGG3650.017.9714329
ATCGTGC450.00884104917.7812588
CGGCATC3750.017.49219325
TAGCGGC3750.017.49219330
GTATAGA1302.582965E-1017.2302881
CATCGTT3900.017.22964528
GGCATCG3900.017.22964526
CGTTTAT4050.016.98654631
GCATCGT3950.016.6065127
GTGTTAC908.585217E-615.9995531
CCGGTCC4650.015.8304649
ATACCGT3600.015.5591816
TAAGACT1551.891749E-1015.4857474
CTAGACA1451.2187229E-915.4501484
Produced by FastQC (version 0.11.5)