##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062830_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1341376
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173825981678515	32.0	32.0	32.0	32.0	32.0
2	31.32324717305215	32.0	32.0	32.0	32.0	32.0
3	31.409386331647504	32.0	32.0	32.0	32.0	32.0
4	31.50702487594828	32.0	32.0	32.0	32.0	32.0
5	31.435501306121477	32.0	32.0	32.0	32.0	32.0
6	34.955444260222336	36.0	36.0	36.0	36.0	36.0
7	34.982148927668305	36.0	36.0	36.0	36.0	36.0
8	34.93153672050193	36.0	36.0	36.0	36.0	36.0
9	35.02465080633618	36.0	36.0	36.0	36.0	36.0
10	34.88773319337755	36.0	36.0	36.0	32.0	36.0
11	35.04706733980629	36.0	36.0	36.0	36.0	36.0
12	34.950960058924565	36.0	36.0	36.0	36.0	36.0
13	34.99611518321485	36.0	36.0	36.0	36.0	36.0
14	34.951957542106015	36.0	36.0	36.0	32.0	36.0
15	34.925656191850756	36.0	36.0	36.0	32.0	36.0
16	34.932994924614725	36.0	36.0	36.0	36.0	36.0
17	34.904685934443435	36.0	36.0	36.0	32.0	36.0
18	34.89607462784484	36.0	36.0	36.0	32.0	36.0
19	34.88858903096522	36.0	36.0	36.0	32.0	36.0
20	34.87027425568968	36.0	36.0	36.0	32.0	36.0
21	34.85467460279593	36.0	36.0	36.0	32.0	36.0
22	34.83964824180543	36.0	36.0	36.0	32.0	36.0
23	34.78814963142325	36.0	36.0	36.0	32.0	36.0
24	34.76496597523737	36.0	36.0	36.0	32.0	36.0
25	34.745203432892794	36.0	36.0	36.0	32.0	36.0
26	34.68227327758958	36.0	36.0	36.0	32.0	36.0
27	34.667141055155305	36.0	36.0	36.0	32.0	36.0
28	34.63820509685576	36.0	36.0	36.0	32.0	36.0
29	34.59947322749177	36.0	36.0	36.0	32.0	36.0
30	34.571651050861206	36.0	36.0	36.0	32.0	36.0
31	34.563464681043946	36.0	36.0	36.0	32.0	36.0
32	34.53199326661577	36.0	36.0	36.0	32.0	36.0
33	34.4867673195286	36.0	36.0	36.0	32.0	36.0
34	34.47285772221957	36.0	36.0	36.0	32.0	36.0
35	34.42503816976001	36.0	36.0	36.0	32.0	36.0
36	34.394616423732046	36.0	36.0	36.0	32.0	36.0
37	34.376256918269	36.0	36.0	36.0	32.0	36.0
38	33.95754956104776	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	4.0
21	18.0
22	100.0
23	317.0
24	841.0
25	1975.0
26	4492.0
27	8610.0
28	15301.0
29	24259.0
30	37134.0
31	53400.0
32	75865.0
33	120960.0
34	294444.0
35	703653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69948502916329	18.786482071454717	12.462488098708583	26.051544800673405
2	15.153767474593252	21.53184491149387	37.77441970036738	25.539967913545492
3	17.622128321961924	25.754523712963405	29.487630612147527	27.13571735292714
4	11.660348522970834	16.988836082378157	36.925020967291026	34.42579442735998
5	13.408171907056635	37.80289791974808	33.61346855766019	15.175461615535093
6	32.47856302567367	37.01905657929854	17.25760302495799	13.244777370069794
7	28.63509491802399	31.605047980867297	21.599143560902004	18.160713540206714
8	27.39128587180422	33.507636771912246	20.039676770749228	19.061400585534304
9	27.550573089614304	14.152115145758748	18.907633192825607	39.38967857180133
10	15.554230205797825	27.33103964990886	31.97325112851461	25.1414790157787
11	36.77717620640244	21.523788080536672	22.86822803514794	18.830807677912954
12	24.414740153723432	24.52134757747676	29.418803163929415	21.645109104870393
13	29.469058941845404	19.943744208359643	25.64312834685058	24.944068502944372
14	23.285566463094614	20.471814017844363	26.1304809389761	30.11213858008493
15	24.67585524118517	27.858929934634286	23.344163008731332	24.12105181544921
16	24.633510663676702	26.240740851185645	25.03160933250632	24.094139152631328
17	23.128787155875756	26.226427191182783	26.34958430745742	24.295201345484042
18	24.035020754807004	24.95914642874183	27.8795058208884	23.126326995562767
19	24.966154158118233	25.346435302256786	26.612970561572595	23.07443997805239
20	25.169303759721362	24.51795767927859	26.5080037215516	23.804734839448447
21	26.63097689384169	24.457608392588494	25.29011297371203	23.621301739857785
22	24.917323703420966	25.03272758719405	26.13711591678992	23.91283279259507
23	23.44146864225142	24.614444133817912	26.624172956853975	25.319914267076694
24	23.95808483229162	25.651569731380313	26.44605241185171	23.94429302447636
25	24.227154971577672	24.872015655577297	26.496468176311623	24.404361196533408
26	23.51965444439143	25.94209229925092	27.08733420010497	23.450919056252683
27	24.558084055540025	25.324536389898427	26.14965986394558	23.967719690615972
28	23.406561620306313	25.413977885395294	27.01315663915263	24.16630385514576
29	23.629317954100866	25.225887446920176	26.914153824132832	24.23064077484613
30	23.622608425974523	25.591183978243237	27.0930000238561	23.69320757192614
31	24.179648361085928	25.274270599742355	25.98220036738394	24.56388067178778
32	23.669053270671313	25.722467078581996	25.977280046757954	24.63119960398874
33	23.463965360942794	25.08983312658047	26.77019717066654	24.676004341810202
34	24.033380647931676	25.40055882914261	26.904164082255832	23.66189644066988
35	24.94580192280166	25.281725630993847	26.578826518440767	23.19364592776373
36	23.67039517629658	26.08135228302877	26.189226585237847	24.059025955436805
37	24.567757288038553	25.889236127677844	25.942688701751038	23.600317882532565
38	23.877645045088027	25.67005820888401	26.388574120902714	24.063722625125244
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	601.0
1	462.5
2	324.0
3	324.0
4	994.5
5	1665.0
6	1665.0
7	1995.5
8	2326.0
9	2258.0
10	2190.0
11	2190.0
12	2727.5
13	3265.0
14	4039.0
15	4813.0
16	4813.0
17	7283.5
18	9754.0
19	9754.0
20	11159.5
21	12565.0
22	12949.0
23	13333.0
24	13333.0
25	15468.0
26	17603.0
27	17603.0
28	22644.5
29	27686.0
30	33547.5
31	39409.0
32	39409.0
33	48338.0
34	57267.0
35	57267.0
36	61939.0
37	66611.0
38	74838.0
39	83065.0
40	83065.0
41	86436.0
42	89807.0
43	99031.0
44	108255.0
45	108255.0
46	111453.0
47	114651.0
48	114651.0
49	118969.0
50	123287.0
51	119871.0
52	116455.0
53	116455.0
54	108879.0
55	101303.0
56	101303.0
57	97049.5
58	92796.0
59	81935.5
60	71075.0
61	71075.0
62	68133.5
63	65192.0
64	53400.5
65	41609.0
66	41609.0
67	35189.5
68	28770.0
69	28770.0
70	23446.5
71	18123.0
72	14267.0
73	10411.0
74	10411.0
75	7810.0
76	5209.0
77	5209.0
78	5264.0
79	5319.0
80	4045.5
81	2772.0
82	2772.0
83	2660.5
84	2549.0
85	2549.0
86	1621.5
87	694.0
88	587.0
89	480.0
90	480.0
91	286.0
92	92.0
93	56.0
94	20.0
95	20.0
96	19.0
97	18.0
98	18.0
99	15.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01006429218951286
2	0.0
3	0.0
4	7.455031251491006E-5
5	0.0
6	1.4910062502982013E-4
7	5.964025001192805E-4
8	0.002758361563051672
9	0.0037275156257455034
10	8.200534376640107E-4
11	0.005516723126103344
12	4.4730187508946036E-4
13	2.2365093754473018E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.4910062502982013E-4
22	0.0
23	7.455031251491006E-5
24	0.0
25	7.455031251491006E-5
26	0.0
27	7.455031251491006E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1341376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.52601845384287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.17711344792781	40.538064851801806
2	14.37946964707062	15.105925760770095
3	4.107428684645511	6.47240624662601
4	1.6426199066518328	3.4512113411777525
5	0.8039154562502319	2.1113239045164596
6	0.4141938572918391	1.3053572514947704
7	0.2696705141850837	0.9915302883180103
8	0.19361561370754232	0.8135885838843585
9	0.12972431573156923	0.6132511621825691
>10	0.688988094461074	6.754105045131882
>50	0.08655931742287121	3.219335861320624
>100	0.08946372841427606	10.05742213551511
>500	0.012821218690821335	4.557593840716222
>1k	0.004273739563607112	3.525423199111841
>5k	1.4245798545357038E-4	0.4834605274326444
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6461	0.4816695691588339	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4916	0.3664893363232979	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2859	0.2131393434801279	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2155	0.1606559234696312	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1989	0.14828057159215613	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1796	0.13389236127677848	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1739	0.12964299346342859	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1647	0.12278436471205686	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1566	0.11674578939834916	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1561	0.11637303783577461	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1553	0.11577663533565531	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1518	0.11316737439763347	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1514	0.11286917314757383	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1484	0.11063266377212652	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1413	0.10533959158356791	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1406	0.10481773939596355	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1380	0.10287943127057589	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.455031251491006E-5	0.0
6	0.0	0.0	0.0	7.455031251491006E-5	0.0
7	0.0	0.0	0.0	7.455031251491006E-5	0.0
8	0.0	0.0	0.0	7.455031251491006E-5	0.0
9	0.0	0.0	0.0	7.455031251491006E-5	0.0
10	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5	0.0
11	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5	0.0
12	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5	1.4910062502982013E-4
13	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5	1.4910062502982013E-4
14	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5	1.4910062502982013E-4
15	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5	1.4910062502982013E-4
16	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5	1.4910062502982013E-4
17	0.0	0.0	7.455031251491006E-5	7.455031251491006E-5	1.4910062502982013E-4
18	0.0	0.0	7.455031251491006E-5	1.4910062502982013E-4	2.2365093754473018E-4
19	0.0	0.0	7.455031251491006E-5	1.4910062502982013E-4	2.2365093754473018E-4
20	0.0	0.0	7.455031251491006E-5	2.2365093754473018E-4	2.2365093754473018E-4
21	0.0	0.0	7.455031251491006E-5	5.218521876043705E-4	2.2365093754473018E-4
22	0.0	0.0	7.455031251491006E-5	0.001043704375208741	2.2365093754473018E-4
23	0.0	0.0	7.455031251491006E-5	0.002311059687962212	2.2365093754473018E-4
24	0.0	0.0	7.455031251491006E-5	0.003876616250775323	2.2365093754473018E-4
25	0.0	0.0	7.455031251491006E-5	0.004696669688439334	2.2365093754473018E-4
26	7.455031251491006E-5	0.0	7.455031251491006E-5	0.006336776563767355	2.2365093754473018E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	30	8.4061513E-4	26.672882	5
TTACACG	35	0.002065264	22.859913	4
GTAATCG	35	0.0020675033	22.855652	31
TCTAGCG	360	0.0	18.66545	28
TCGTTTA	370	0.0	18.160976	30
ATCGTTT	370	0.0	18.160976	29
CTAGCGG	365	0.0	17.97143	29
ATCGTGC	45	0.008841049	17.781258	8
CGGCATC	375	0.0	17.492193	25
TAGCGGC	375	0.0	17.492193	30
GTATAGA	130	2.582965E-10	17.230288	1
CATCGTT	390	0.0	17.229645	28
GGCATCG	390	0.0	17.229645	26
CGTTTAT	405	0.0	16.986546	31
GCATCGT	395	0.0	16.60651	27
GTGTTAC	90	8.585217E-6	15.999553	1
CCGGTCC	465	0.0	15.830464	9
ATACCGT	360	0.0	15.559181	6
TAAGACT	155	1.891749E-10	15.485747	4
CTAGACA	145	1.2187229E-9	15.450148	4
>>END_MODULE