FastQCFastQC Report
Thu 2 Feb 2017
SRR4062829_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062829_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771597
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49150.17733458363535537No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC42690.154026721778094No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT41840.15095989784950697No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA40630.14659418378645958No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG39430.14226455000492497No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC38470.13880084297969725No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG37780.13631130355531484No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG37620.13573401905111024No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC37270.13447120919816263No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA33870.12220391348381457No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT33790.12191527123171227No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG33430.12061638109725187No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG33200.11978653462245774No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG32400.11690011210143465No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC32300.11653930928630678No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC32250.11635890787874284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31420.11336424451318138No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT30060.10845732622744216No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC29400.10607602764759812No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA29100.10499361920221448No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG28510.10286488259295995No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA28370.10235975865178092No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT28290.10207111639967859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG8650.019.97660828
CTAGCGG9150.018.88498929
TAGCGGC9550.017.7589230
GTATCAA41700.017.5538581
AGCGGCG11050.015.34820831
ATACCGT8500.015.243556
CGCTTCG8500.015.058619532
GCGGCGC11450.014.95176232
CGGTCCA12000.014.80006710
ACCGTCG8100.014.6162968
GTAAACG9250.014.52953327
AACGCTT9200.014.434587530
GTCCTAC4350.014.360441
ACGCTGA9700.014.35058111
TAAACGC9150.014.33860328
AAACGCT9200.014.26067629
ACGCTTC9000.014.2220331
CGCCGGT11600.014.2059367
CCGTCGT8450.014.2012479
TACCGTC8600.014.1385777
Produced by FastQC (version 0.11.5)