##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062829_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2771597 Sequences flagged as poor quality 0 Sequence length 38 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.224472749826184 32.0 32.0 32.0 32.0 32.0 2 30.776547600534997 32.0 32.0 32.0 32.0 32.0 3 30.821596718426235 32.0 32.0 32.0 32.0 32.0 4 30.907021475344358 32.0 32.0 32.0 32.0 32.0 5 30.788890664840523 32.0 32.0 32.0 32.0 32.0 6 34.45765852683489 36.0 36.0 36.0 32.0 36.0 7 34.3846103888841 36.0 36.0 36.0 32.0 36.0 8 34.36697831611161 36.0 36.0 36.0 32.0 36.0 9 34.53805333170732 36.0 36.0 36.0 32.0 36.0 10 34.20144162372812 36.0 36.0 36.0 32.0 36.0 11 34.51030362639302 36.0 36.0 36.0 32.0 36.0 12 34.293832761400736 36.0 36.0 36.0 32.0 36.0 13 34.403665107156634 36.0 36.0 36.0 32.0 36.0 14 34.28764607552974 36.0 36.0 36.0 32.0 36.0 15 34.21767775040888 36.0 36.0 36.0 32.0 36.0 16 34.24278132787703 36.0 36.0 36.0 32.0 36.0 17 34.15031983365547 36.0 36.0 36.0 32.0 36.0 18 34.18904010936655 36.0 36.0 36.0 32.0 36.0 19 34.17760266012699 36.0 36.0 36.0 32.0 36.0 20 34.17183486632436 36.0 36.0 36.0 32.0 36.0 21 34.15940809576573 36.0 36.0 36.0 32.0 36.0 22 34.13575386320594 36.0 36.0 36.0 32.0 36.0 23 34.067864844708666 36.0 36.0 36.0 32.0 36.0 24 34.0502165358095 36.0 36.0 36.0 32.0 36.0 25 34.02486328279328 36.0 36.0 36.0 32.0 36.0 26 33.973421460623605 36.0 36.0 36.0 32.0 36.0 27 33.97850264666905 36.0 36.0 36.0 32.0 36.0 28 33.96417336286625 36.0 36.0 36.0 32.0 36.0 29 33.93735597202624 36.0 36.0 36.0 32.0 36.0 30 33.90874466958941 36.0 36.0 36.0 32.0 36.0 31 33.9330382447376 36.0 36.0 36.0 32.0 36.0 32 33.8819081562002 36.0 36.0 36.0 32.0 36.0 33 33.83844909631523 36.0 36.0 36.0 32.0 36.0 34 33.86284441785729 36.0 36.0 36.0 32.0 36.0 35 33.82435577755352 36.0 36.0 36.0 32.0 36.0 36 33.77137946101111 36.0 36.0 36.0 32.0 36.0 37 33.77984389505401 36.0 36.0 36.0 32.0 36.0 38 33.184057061686815 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 2.0 8 2.0 9 6.0 10 24.0 11 16.0 12 13.0 13 15.0 14 205.0 15 514.0 16 759.0 17 871.0 18 1145.0 19 1595.0 20 2360.0 21 3354.0 22 5388.0 23 8804.0 24 13345.0 25 20424.0 26 29462.0 27 42596.0 28 58954.0 29 78400.0 30 103850.0 31 139243.0 32 187708.0 33 277642.0 34 602158.0 35 1192741.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.41131941612305 17.264567754557543 11.41008473360948 25.91402809570993 2 17.00307461935476 19.334110441540176 37.10824053580756 26.554574403297497 3 18.792417589713832 23.418623180247515 28.34227512723473 29.44668410280393 4 12.59168254243159 15.05011690335989 35.96510218219605 36.393098372012474 5 15.07869845200881 36.06003336710454 33.310169750814694 15.551098430071958 6 34.85038180277877 35.337286782884036 16.530775858460924 13.28155555587627 7 30.877613159488916 30.092397992926102 20.741651834664275 18.28833701292071 8 28.572351197434543 32.40112541817122 19.294903434560354 19.73161994983389 9 27.537691306879726 14.064361519039226 18.53337826535921 39.86456890872184 10 16.234868793724903 25.870096226353827 30.959449269192053 26.93558571072922 11 38.14505709245742 20.92120061034285 21.927566346281168 19.006175950918564 12 25.10606009217973 23.501032998958703 28.104607471219996 23.288299437641573 13 29.262866626178514 18.83640945863875 25.365286496984496 26.53543741819823 14 23.905121538897316 19.713278979264505 24.532544784372874 31.849054697465302 15 25.412181555125773 26.908323459982885 21.902814473279157 25.776680511612177 16 26.36497804271657 25.458613136842413 23.212402888185647 24.964005932255375 17 24.549532480936552 25.696298461571775 24.60983540297581 25.14433365451586 18 25.68441486511598 24.22399064751187 25.72583715052202 24.365757336850137 19 25.9148284220865 24.707060778799406 24.99785306600216 24.38025773311193 20 26.060274542009026 23.88985990015342 24.41402557215496 25.635839985682594 21 27.659568326425987 23.80360288455918 23.92527222251489 24.611556566499942 22 26.24396559282875 23.79527451658891 24.67789733632725 25.282862554255086 23 24.50377580712207 23.57295852385571 25.378374555447785 26.54489111357443 24 25.110935895886072 24.688843621614502 24.812508683077155 25.38771179942227 25 25.223464795949667 24.13910570777986 24.864008545250492 25.773420951019983 26 25.043259665896443 25.147912872236116 25.323621996949182 24.485205464918263 27 25.928354206388498 24.28681122165467 24.288038057903787 25.49679651405305 28 24.806022258368536 24.318768645460935 25.501145248050484 25.37406384812005 29 24.82257501140254 24.559234037885304 25.34319484778329 25.274996102928863 30 24.675360287840597 24.824880373107455 25.70212019769557 24.797639141356377 31 25.58666837455416 24.65517210274026 24.10491961803457 25.65323990467101 32 25.19199571626923 24.4636315876072 24.031109007092404 26.313263689031167 33 24.65811998194393 24.062132514387304 24.936538538562377 26.343208965106392 34 25.774516507923334 24.258663524877807 25.018213835297853 24.948606131901006 35 26.42820386426082 23.899639786975605 25.145120885354682 24.527035463408893 36 24.82340472443559 24.837730818821097 24.662209098188598 25.67665535855472 37 26.18292181715901 24.572616033344193 24.483128540484252 24.761333609012546 38 24.985828702479246 24.23033888073631 25.123265932922408 25.66056648386204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 179.5 2 239.0 3 239.0 4 563.0 5 887.0 6 887.0 7 1080.0 8 1273.0 9 1275.5 10 1278.0 11 1278.0 12 1810.0 13 2342.0 14 3312.5 15 4283.0 16 4283.0 17 6563.0 18 8843.0 19 8843.0 20 11136.0 21 13429.0 22 15232.5 23 17036.0 24 17036.0 25 20434.0 26 23832.0 27 23832.0 28 30578.5 29 37325.0 30 45779.0 31 54233.0 32 54233.0 33 72022.0 34 89811.0 35 89811.0 36 101030.0 37 112249.0 38 129403.0 39 146557.0 40 146557.0 41 157176.5 42 167796.0 43 192861.0 44 217926.0 45 217926.0 46 221374.0 47 224822.0 48 224822.0 49 243324.0 50 261826.0 51 266204.5 52 270583.0 53 270583.0 54 257318.0 55 244053.0 56 244053.0 57 236943.0 58 229833.0 59 205373.0 60 180913.0 61 180913.0 62 172670.0 63 164427.0 64 136326.0 65 108225.0 66 108225.0 67 90786.5 68 73348.0 69 73348.0 70 58796.5 71 44245.0 72 34455.0 73 24665.0 74 24665.0 75 18382.5 76 12100.0 77 12100.0 78 11943.5 79 11787.0 80 9316.0 81 6845.0 82 6845.0 83 6916.0 84 6987.0 85 6987.0 86 4736.0 87 2485.0 88 2110.0 89 1735.0 90 1735.0 91 1274.0 92 813.0 93 692.5 94 572.0 95 572.0 96 538.0 97 504.0 98 504.0 99 937.0 100 1370.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11812684167286946 2 0.05404826170615713 3 0.01230337599586087 4 0.0027781816764847127 5 1.8040140756394238E-4 6 3.247225336150963E-4 7 0.0 8 1.8040140756394238E-4 9 1.0824084453836544E-4 10 2.5256197058951934E-4 11 0.001443211260511539 12 0.001984415483203366 13 0.015009397109320007 14 0.00945303375635058 15 0.02417378861356828 16 0.011076646424426061 17 0.021179125248006834 18 0.005123399974815963 19 0.007324297147096061 20 0.005231640819354329 21 0.005448122508431059 22 0.007143895739532119 23 0.0097055957269401 24 0.014504273168140968 25 0.01684949146647222 26 0.019483352016905778 27 0.008695347844582023 28 0.005231640819354329 29 0.010282880231144716 30 0.0022730577353056738 31 0.005123399974815963 32 0.0065305309538147135 33 0.00829846474794135 34 0.012700259092501543 35 0.01656084921436991 36 0.015514521050499046 37 0.009380873193325003 38 0.005412042226918271 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 2771597.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.79924048980701 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3078421703318 38.21363222307872 2 12.687614633460734 12.3829191548049 3 3.8885676036123105 5.692774369486491 4 1.6758028764954345 3.271116303344442 5 0.9187953755018949 2.241825824501975 6 0.5530561778711532 1.6193232856984734 7 0.36042905488658505 1.2312064890247019 8 0.24162201158061344 0.943277652060263 9 0.1942657465137607 0.8532018794751206 >10 1.0024821348697186 8.679143003450331 >50 0.07192972927333542 2.4549290857846504 >100 0.07135045905945708 7.57862824136766 >500 0.01583442394821809 5.383306939216955 >1k 0.010407602595067879 9.454715548705378 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 4915 0.17733458363535537 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC 4269 0.154026721778094 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT 4184 0.15095989784950697 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA 4063 0.14659418378645958 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG 3943 0.14226455000492497 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC 3847 0.13880084297969725 No Hit GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG 3778 0.13631130355531484 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG 3762 0.13573401905111024 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC 3727 0.13447120919816263 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 3387 0.12220391348381457 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT 3379 0.12191527123171227 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG 3343 0.12061638109725187 No Hit GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG 3320 0.11978653462245774 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG 3240 0.11690011210143465 No Hit GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC 3230 0.11653930928630678 No Hit TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC 3225 0.11635890787874284 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 3142 0.11336424451318138 No Hit CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT 3006 0.10845732622744216 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC 2940 0.10607602764759812 No Hit GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA 2910 0.10499361920221448 No Hit GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG 2851 0.10286488259295995 No Hit ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA 2837 0.10235975865178092 No Hit CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT 2829 0.10207111639967859 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6080281512788477E-5 0.0 5 0.0 0.0 0.0 3.6080281512788477E-5 0.0 6 0.0 0.0 0.0 3.6080281512788477E-5 0.0 7 0.0 0.0 0.0 7.216056302557695E-5 0.0 8 0.0 0.0 0.0 7.216056302557695E-5 0.0 9 0.0 0.0 0.0 1.443211260511539E-4 0.0 10 0.0 0.0 0.0 1.443211260511539E-4 0.0 11 0.0 0.0 0.0 1.443211260511539E-4 0.0 12 0.0 0.0 0.0 1.443211260511539E-4 0.0 13 0.0 0.0 0.0 1.443211260511539E-4 0.0 14 0.0 0.0 0.0 1.443211260511539E-4 0.0 15 0.0 0.0 0.0 1.443211260511539E-4 0.0 16 0.0 0.0 0.0 1.443211260511539E-4 0.0 17 0.0 0.0 0.0 1.443211260511539E-4 0.0 18 0.0 0.0 0.0 1.8040140756394238E-4 3.6080281512788477E-5 19 0.0 0.0 0.0 2.5256197058951934E-4 3.6080281512788477E-5 20 0.0 0.0 0.0 3.247225336150963E-4 1.443211260511539E-4 21 0.0 0.0 0.0 3.968830966406732E-4 1.8040140756394238E-4 22 0.0 0.0 0.0 4.6904365966625016E-4 1.8040140756394238E-4 23 0.0 0.0 0.0 5.772845042046156E-4 1.8040140756394238E-4 24 0.0 0.0 0.0 7.937661932813464E-4 1.8040140756394238E-4 25 0.0 0.0 0.0 9.380873193325003E-4 1.8040140756394238E-4 26 0.0 0.0 0.0 0.0011545690084092312 1.8040140756394238E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTAGCG 865 0.0 19.976608 28 CTAGCGG 915 0.0 18.884989 29 TAGCGGC 955 0.0 17.75892 30 GTATCAA 4170 0.0 17.553858 1 AGCGGCG 1105 0.0 15.348208 31 ATACCGT 850 0.0 15.24355 6 CGCTTCG 850 0.0 15.0586195 32 GCGGCGC 1145 0.0 14.951762 32 CGGTCCA 1200 0.0 14.800067 10 ACCGTCG 810 0.0 14.616296 8 GTAAACG 925 0.0 14.529533 27 AACGCTT 920 0.0 14.4345875 30 GTCCTAC 435 0.0 14.36044 1 ACGCTGA 970 0.0 14.350581 11 TAAACGC 915 0.0 14.338603 28 AAACGCT 920 0.0 14.260676 29 ACGCTTC 900 0.0 14.22203 31 CGCCGGT 1160 0.0 14.205936 7 CCGTCGT 845 0.0 14.201247 9 TACCGTC 860 0.0 14.138577 7 >>END_MODULE