FastQCFastQC Report
Thu 2 Feb 2017
SRR4062829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771597
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50420.1819167793874795No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC46370.16730426537480014No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT44130.15922228231593555No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG42850.15460400628229862No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC42820.15449576543776025No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA41840.15095989784950697No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC38660.13948636832844025No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG37860.13659994580741716No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA37020.13356920216034293No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC36630.13216207118134418No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG36040.13003333457208965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35520.12815715993342466No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG34900.12592018247963177No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA33600.12122974588296928No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG33490.1208328627863286No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT33200.11978653462245774No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG31870.11498785718125687No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC31490.11361680648377091No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG29950.10806044313080149No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT29590.10676155299634109No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG28840.10405553188288195No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT28650.10337000653413898No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA28500.10282880231144716No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT28240.10189071499211466No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT27990.10098870795429493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA701.7874572E-518.2872419
TCTAGCG9050.018.20916628
TAGCGGC9400.017.70136830
CTAGCGG9450.017.6077129
GTATCAA43250.016.982071
ATCGTTT6850.015.88253229
AGCGGCG10500.015.54219131
GTATTAG6950.015.4260271
CATCGTT7400.015.13448728
TCGTTTA7350.015.01976430
TTTTCGG9800.014.85650629
AAGACGG12000.014.8015025
CGGTCCA11700.014.76966410
GTTTTCG9950.014.63253928
TAAGGCG1103.5428457E-614.546935
CAAGACG12000.014.5342844
TACACCG1553.1341187E-914.4530795
GTATTAC2003.6379788E-1214.4014621
ATACCGT8650.014.2444776
CGCAAGA11350.014.2383032
Produced by FastQC (version 0.11.5)