FastQCFastQC Report
Thu 2 Feb 2017
SRR4062828_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062828_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784595
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58040.3252278528181464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38010.2129895018197406No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25200.1412085094937507No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24790.13891106945833648No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24630.1380145074932968No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24100.13504464598410282No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23590.13218685472053884No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22800.1277600800181554No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22450.12579885071963107No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22150.12411779703518165No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21960.12305312970169703No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21780.12204449749102739No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20750.11627287984108439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20620.11554442324448964No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20450.11459182615663499No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19460.10904434899795191No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19260.10792364654165232No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT18870.10573827675186807No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18600.1042253284358636No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT18280.10243220450578422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG3050.017.310497
CATCGTT4850.017.15414428
GTGTATA2400.016.6830181
ATCGTTT4800.016.6671229
CGCTTCG6100.016.52503832
ACCGTCG4400.016.3627058
TACCGTC4550.016.1749047
AAACGCT6200.016.00043529
GTCGATA703.6978515E-415.9999869
CTAGCGG5550.015.85628629
TCTAGCG5550.015.85539828
CGTTTAT5200.015.69273431
TAATACC1551.891749E-1015.4803874
AACGCTT6350.015.37049630
CGTCGTA4700.015.31956610
AGAACCG4000.015.1961545
ATACCGT4950.015.1885016
GTACTAA951.4200354E-515.1727651
GTAAACG6550.015.14375227
TCGTTTA5400.015.11152230
Produced by FastQC (version 0.11.5)