##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062828_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1784595
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249041379136443	32.0	32.0	32.0	32.0	32.0
2	30.913237457238196	32.0	32.0	32.0	32.0	32.0
3	30.970978289191667	32.0	32.0	32.0	32.0	32.0
4	31.018440038215953	32.0	32.0	32.0	32.0	32.0
5	30.963916743014522	32.0	32.0	32.0	32.0	32.0
6	34.600658973044304	36.0	36.0	36.0	32.0	36.0
7	34.53027661738378	36.0	36.0	36.0	32.0	36.0
8	34.520166200174266	36.0	36.0	36.0	32.0	36.0
9	34.6294509398491	36.0	36.0	36.0	32.0	36.0
10	34.38937854247042	36.0	36.0	36.0	32.0	36.0
11	34.62797721611906	36.0	36.0	36.0	32.0	36.0
12	34.46507302777381	36.0	36.0	36.0	32.0	36.0
13	34.53892395753658	36.0	36.0	36.0	32.0	36.0
14	34.44151474143993	36.0	36.0	36.0	32.0	36.0
15	34.39300121316041	36.0	36.0	36.0	32.0	36.0
16	34.41012106388284	36.0	36.0	36.0	32.0	36.0
17	34.34445294310474	36.0	36.0	36.0	32.0	36.0
18	34.36225866373043	36.0	36.0	36.0	32.0	36.0
19	34.35444120374651	36.0	36.0	36.0	32.0	36.0
20	34.31831479971646	36.0	36.0	36.0	32.0	36.0
21	34.29986187342226	36.0	36.0	36.0	32.0	36.0
22	34.2803706163023	36.0	36.0	36.0	32.0	36.0
23	34.23254127687235	36.0	36.0	36.0	32.0	36.0
24	34.216748898209396	36.0	36.0	36.0	32.0	36.0
25	34.19126860716297	36.0	36.0	36.0	32.0	36.0
26	34.14222498662161	36.0	36.0	36.0	32.0	36.0
27	34.144829499130054	36.0	36.0	36.0	32.0	36.0
28	34.13019088364587	36.0	36.0	36.0	32.0	36.0
29	34.092457952644715	36.0	36.0	36.0	32.0	36.0
30	34.06820314973425	36.0	36.0	36.0	32.0	36.0
31	34.08361785166942	36.0	36.0	36.0	32.0	36.0
32	34.0407616293893	36.0	36.0	36.0	32.0	36.0
33	33.99761794692913	36.0	36.0	36.0	32.0	36.0
34	33.98774343758668	36.0	36.0	36.0	32.0	36.0
35	33.94981102154831	36.0	36.0	36.0	32.0	36.0
36	33.90839097946593	36.0	36.0	36.0	32.0	36.0
37	33.902646258674935	36.0	36.0	36.0	32.0	36.0
38	33.29805642176516	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	5.0
10	15.0
11	7.0
12	12.0
13	4.0
14	295.0
15	553.0
16	739.0
17	844.0
18	1005.0
19	1275.0
20	1755.0
21	2397.0
22	3529.0
23	5182.0
24	7421.0
25	10922.0
26	16128.0
27	22940.0
28	32479.0
29	44253.0
30	60163.0
31	81248.0
32	111082.0
33	167655.0
34	382472.0
35	830211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.93413671201965	16.933452798463186	11.35548973877195	25.77692075074521
2	16.767399380353645	19.902578944358538	37.26220818816796	26.067813487119857
3	18.86351408908516	24.504416149207557	28.19212490752987	28.43994485417741
4	12.637837413772814	15.462446694647891	35.79844552907488	36.10127036250441
5	14.811404966179925	36.40638106994824	33.202341600960665	15.579872362911166
6	33.93917013713	35.38987237361761	17.009286197791752	13.661671291460642
7	30.140973240972457	30.409885946047112	21.185434894435375	18.26370591854506
8	28.45249782583358	32.20024521010965	19.671331038130845	19.675925925925927
9	27.97797593064637	14.037598488843114	18.446166717004942	39.53825886350557
10	16.162078593918718	26.182862067554975	31.023579072179565	26.631480266346742
11	37.660984790113886	21.012259023637718	22.276994423844943	19.049761762403456
12	25.620447404652914	23.43643443562247	28.30452789736848	22.638590262356136
13	29.839330218461363	19.178599630228042	25.119609850796625	25.862460300513973
14	24.259514760389543	19.75204518173229	24.695618452420316	31.292821605457853
15	25.370856432166917	26.793077608157468	22.55129944775782	25.284766511917795
16	25.997071286106653	25.24225216192997	23.76954507422939	24.991131477733987
17	24.255562891414467	25.401825096932352	25.170297169541662	25.172314842111515
18	25.391844259768785	24.03388044897479	26.379958644109585	24.194316647146834
19	25.92424891116496	24.624539640768496	25.322335520343227	24.12887592772332
20	26.03755483377828	23.666056966607716	25.098796066538004	25.197592133076004
21	27.528669159247205	23.69868018757117	24.123281867821504	24.649368785360124
22	26.162892676795273	24.057987582735528	24.70670250962341	25.07241723084579
23	24.271806574251745	23.790864910424474	25.602998403959614	26.334330111364167
24	25.047958559769285	24.931613867883765	25.041401561238164	24.979026011108786
25	25.1104489188812	24.104233067254608	25.205667852373104	25.579650161491085
26	24.74377490863215	25.085034728468937	25.64285357997748	24.52833678292143
27	25.48654028499761	24.576729005691433	24.688921317435874	25.24780939187508
28	24.506503185748468	24.343545286941513	25.66822264935473	25.481728877955295
29	24.52645571436257	24.736104137151873	25.615775693560245	25.12166445492531
30	24.525370009660712	24.87425387268933	25.88644295362187	24.71393316402809
31	25.30582967682643	24.708852389198036	24.36046153242663	25.6248564015489
32	24.66187534148699	24.843049076058783	24.47610641785399	26.018969164600232
33	24.348763654051776	24.354759892672934	25.303566585147706	25.992909868127583
34	25.318671144828713	24.534581808746314	25.38984500351106	24.756902042913914
35	26.06149291908381	24.266898615313654	25.404653805995952	24.26695465960658
36	24.619370559650115	25.28667350016561	24.816753691966863	25.277202248217417
37	25.690138666594937	25.0366504821724	24.64117272576473	24.63203812546794
38	24.746875308210274	24.959428320377295	25.10081277851001	25.192883592902422
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	782.0
1	622.5
2	463.0
3	463.0
4	1042.5
5	1622.0
6	1622.0
7	1894.5
8	2167.0
9	2033.5
10	1900.0
11	1900.0
12	2377.5
13	2855.0
14	3603.0
15	4351.0
16	4351.0
17	6436.0
18	8521.0
19	8521.0
20	10113.5
21	11706.0
22	11697.5
23	11689.0
24	11689.0
25	13357.0
26	15025.0
27	15025.0
28	19851.0
29	24677.0
30	31055.0
31	37433.0
32	37433.0
33	48703.0
34	59973.0
35	59973.0
36	66552.5
37	73132.0
38	83904.5
39	94677.0
40	94677.0
41	101153.0
42	107629.0
43	122684.0
44	137739.0
45	137739.0
46	143524.5
47	149310.0
48	149310.0
49	157231.5
50	165153.0
51	165985.0
52	166817.0
53	166817.0
54	159311.0
55	151805.0
56	151805.0
57	148271.5
58	144738.0
59	130501.5
60	116265.0
61	116265.0
62	111303.5
63	106342.0
64	87225.5
65	68109.0
66	68109.0
67	57079.5
68	46050.0
69	46050.0
70	37372.5
71	28695.0
72	22356.0
73	16017.0
74	16017.0
75	11933.5
76	7850.0
77	7850.0
78	7711.0
79	7572.0
80	5985.5
81	4399.0
82	4399.0
83	4509.0
84	4619.0
85	4619.0
86	3038.0
87	1457.0
88	1262.5
89	1068.0
90	1068.0
91	805.5
92	543.0
93	443.5
94	344.0
95	344.0
96	313.5
97	283.0
98	283.0
99	550.5
100	818.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12361348092984684
2	0.05586701744653549
3	0.013168253861520401
4	0.0036422829829737277
5	2.2414049125992172E-4
6	6.163863509647847E-4
7	5.603512281498043E-5
8	1.6810536844494127E-4
9	1.1207024562996086E-4
10	2.2414049125992172E-4
11	0.0017931239300793737
12	0.0020172644213392954
13	0.014120850949375069
14	0.008797514281951926
15	0.023198540845401898
16	0.010870813826106204
17	0.020228679336207936
18	0.004762985439273336
19	0.006668179614982671
20	0.006219898632462827
21	0.005547477158683063
22	0.008069057685357182
23	0.010030286983881496
24	0.013560499721225264
25	0.016026045125084404
26	0.018155379792053658
27	0.00890958452758189
28	0.004762985439273336
29	0.010030286983881496
30	0.00263365077230408
31	0.005883687895572945
32	0.006724214737797651
33	0.008013022562542202
34	0.01300014849307546
35	0.016362255861974283
36	0.013896710458115148
37	0.009582006001361654
38	0.005547477158683063
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1784595.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.857600156581356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91425150733244	35.85216294667489
2	14.435560636045924	13.817025779119424
3	4.805953033138506	6.90004135893756
4	2.037262964683103	3.899940655104619
5	1.0662441360952926	2.551394276727401
6	0.636253924457927	1.826975154885791
7	0.3937114672690174	1.3189460184335122
8	0.28960153491117757	1.1087707570009098
9	0.20302425874670463	0.8744628417467495
>10	1.022207529340218	8.701768019530455
>50	0.08431924576769717	2.861262594502901
>100	0.08898181783797265	9.375034656379272
>500	0.015438857880230002	5.128073222129142
>1k	0.007071232616899238	5.456784294924661
>5k	1.1785387694832061E-4	0.32735742390274464
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5804	0.3252278528181464	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3801	0.2129895018197406	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2520	0.1412085094937507	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2479	0.13891106945833648	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2463	0.1380145074932968	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2410	0.13504464598410282	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2359	0.13218685472053884	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2280	0.1277600800181554	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2245	0.12579885071963107	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2215	0.12411779703518165	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2196	0.12305312970169703	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2178	0.12204449749102739	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2075	0.11627287984108439	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2062	0.11554442324448964	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2045	0.11459182615663499	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1946	0.10904434899795191	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1926	0.10792364654165232	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1887	0.10573827675186807	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1860	0.1042253284358636	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1828	0.10243220450578422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.1207024562996086E-4	0.0
16	0.0	0.0	0.0	2.2414049125992172E-4	5.603512281498043E-5
17	0.0	5.603512281498043E-5	0.0	3.362107368898826E-4	5.603512281498043E-5
18	0.0	5.603512281498043E-5	0.0	4.4828098251984344E-4	5.603512281498043E-5
19	0.0	5.603512281498043E-5	0.0	5.603512281498043E-4	1.1207024562996086E-4
20	0.0	5.603512281498043E-5	0.0	6.724214737797652E-4	1.1207024562996086E-4
21	0.0	1.1207024562996086E-4	0.0	8.965619650396869E-4	1.1207024562996086E-4
22	0.0	1.1207024562996086E-4	0.0	0.0012888078247445499	1.1207024562996086E-4
23	0.0	1.1207024562996086E-4	0.0	0.0025776156494890998	1.1207024562996086E-4
24	0.0	1.1207024562996086E-4	0.0	0.004146599088308551	1.1207024562996086E-4
25	0.0	1.1207024562996086E-4	0.0	0.005099196176163219	1.1207024562996086E-4
26	0.0	1.1207024562996086E-4	0.0	0.006275933755277808	1.1207024562996086E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	305	0.0	17.31049	7
CATCGTT	485	0.0	17.154144	28
GTGTATA	240	0.0	16.683018	1
ATCGTTT	480	0.0	16.66712	29
CGCTTCG	610	0.0	16.525038	32
ACCGTCG	440	0.0	16.362705	8
TACCGTC	455	0.0	16.174904	7
AAACGCT	620	0.0	16.000435	29
GTCGATA	70	3.6978515E-4	15.999986	9
CTAGCGG	555	0.0	15.856286	29
TCTAGCG	555	0.0	15.855398	28
CGTTTAT	520	0.0	15.692734	31
TAATACC	155	1.891749E-10	15.480387	4
AACGCTT	635	0.0	15.370496	30
CGTCGTA	470	0.0	15.319566	10
AGAACCG	400	0.0	15.196154	5
ATACCGT	495	0.0	15.188501	6
GTACTAA	95	1.4200354E-5	15.172765	1
GTAAACG	655	0.0	15.143752	27
TCGTTTA	540	0.0	15.111522	30
>>END_MODULE