FastQCFastQC Report
Thu 2 Feb 2017
SRR4062828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784595
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT67670.3791896760889726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT48330.2708177485648004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT27290.1529198501620816No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26150.14653184616117382No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25390.1422731768272353No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24650.13812657773892675No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24450.13700587528262714No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24300.13616534844040246No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA24060.1348205054928429No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC24030.13465240012439797No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA23690.13274720594868863No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23010.12893681759726996No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22310.12501435900022134No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21950.12299709457888204No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21000.1176737579114589No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20690.1159366691041945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.11515217738478478No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20470.11470389640226494No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19780.11083747292803128No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT19540.10949262998047177No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG19040.10669087383972274No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT18970.10629862798001788No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18600.1042253284358636No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18440.10332876647082391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5100.018.19531428
CTAGCGG5050.018.05864529
TAGCGGC5550.016.43174230
CGGTCCA6250.016.12822710
ATTCGTG1304.80577E-916.00022511
ATAACGA3200.015.99977712
TGTAGGA5850.015.8625812
TAGGACG4050.015.8031334
CGAACGA3350.015.76053116
ACGAACG3350.015.2829415
ACCGTCG5250.015.239598
AGCGGCG6350.015.117475531
CGATAAC3400.015.05903410
TAACGAA3450.014.83995613
TGTCGAC650.00416316614.76902412
CGTCTTA1308.3220584E-814.7686115
CGTCGTA5750.014.74803310
TACCGTC5550.014.70414457
GTAGGAC5550.014.70332053
CCGATAA3500.014.6300079
Produced by FastQC (version 0.11.5)