FastQCFastQC Report
Thu 2 Feb 2017
SRR4062827_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062827_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103468
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2810.2715815517841265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2580.24935245679823712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT1600.15463718251053465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAATA200.00373399732.0067723
GGTATCA502.9522198E-828.8060931
GTATCAA1800.024.0050771
CAACACT350.002057183522.850934
GGGGAGT507.125385E-419.20406220
AACACTG701.7659439E-518.2807435
CTGTAGA450.00878673517.7815381
TCCAGCG450.00881126917.7729432
GTCTGGA550.001351381317.4582391
AGGACAG600.002426204515.995655
AACGCAG2850.015.7150256
TCAACGC2850.015.7150254
CAACGCA2900.015.4440765
TATCAAC2950.015.1823122
ATCAACG2950.015.1823123
GGCAGGT650.004121830714.77235519
CTTCAAG650.004121830714.7723551
CAGGTCC700.006739373313.71718721
TGGGGAG700.006739373313.71718719
AGTACAT2750.013.38464813
Produced by FastQC (version 0.11.5)