##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062827_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	103468
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.326052499323463	32.0	32.0	32.0	32.0	32.0
2	31.02806664862566	32.0	32.0	32.0	32.0	32.0
3	31.054248656589476	32.0	32.0	32.0	32.0	32.0
4	31.11056558549503	32.0	32.0	32.0	32.0	32.0
5	31.046062550740327	32.0	32.0	32.0	32.0	32.0
6	34.70661460548189	36.0	36.0	36.0	32.0	36.0
7	34.651312483086556	36.0	36.0	36.0	32.0	36.0
8	34.64909923841188	36.0	36.0	36.0	32.0	36.0
9	34.776723238102605	36.0	36.0	36.0	32.0	36.0
10	34.54498009046275	36.0	36.0	36.0	32.0	36.0
11	34.72657246684965	36.0	36.0	36.0	32.0	36.0
12	34.613745312560404	36.0	36.0	36.0	32.0	36.0
13	34.65137047203	36.0	36.0	36.0	32.0	36.0
14	34.582566590636716	36.0	36.0	36.0	32.0	36.0
15	34.55255731240577	36.0	36.0	36.0	32.0	36.0
16	34.55599798971663	36.0	36.0	36.0	32.0	36.0
17	34.541191479491246	36.0	36.0	36.0	32.0	36.0
18	34.50345034213477	36.0	36.0	36.0	32.0	36.0
19	34.48435265009471	36.0	36.0	36.0	32.0	36.0
20	34.48877913944408	36.0	36.0	36.0	32.0	36.0
21	34.502396876328916	36.0	36.0	36.0	32.0	36.0
22	34.456653264777515	36.0	36.0	36.0	32.0	36.0
23	34.44564503034755	36.0	36.0	36.0	32.0	36.0
24	34.386370665326474	36.0	36.0	36.0	32.0	36.0
25	34.397514207291145	36.0	36.0	36.0	32.0	36.0
26	34.351867243978816	36.0	36.0	36.0	32.0	36.0
27	34.3554915529439	36.0	36.0	36.0	32.0	36.0
28	34.326168477210345	36.0	36.0	36.0	32.0	36.0
29	34.285595546449144	36.0	36.0	36.0	32.0	36.0
30	34.28688096802876	36.0	36.0	36.0	32.0	36.0
31	34.28683264390923	36.0	36.0	36.0	32.0	36.0
32	34.267889589051684	36.0	36.0	36.0	32.0	36.0
33	34.236179301813124	36.0	36.0	36.0	32.0	36.0
34	34.211350369196275	36.0	36.0	36.0	32.0	36.0
35	34.19313217613175	36.0	36.0	36.0	32.0	36.0
36	34.17445007151969	36.0	36.0	36.0	32.0	36.0
37	34.17970773572505	36.0	36.0	36.0	32.0	36.0
38	33.622028066648625	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	0.0
13	1.0
14	8.0
15	28.0
16	31.0
17	41.0
18	34.0
19	51.0
20	57.0
21	105.0
22	123.0
23	231.0
24	370.0
25	535.0
26	848.0
27	1186.0
28	1684.0
29	2328.0
30	3152.0
31	4315.0
32	5903.0
33	8937.0
34	21512.0
35	51985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07601048954432	17.10647274557049	11.905245739832205	25.91227102505298
2	17.147581565358657	20.21583151217437	37.243506681880596	25.39308024058638
3	18.63032236237978	22.3778454400464	29.49784930646175	29.493982891112076
4	13.724845837118943	15.62699348553092	35.22162726411629	35.42653341323385
5	15.215332276645919	35.49406579812116	33.57269880542776	15.717903119805158
6	34.43286813314261	34.73247767425677	16.784899679127847	14.049754513472765
7	30.459659025012563	30.98059303359493	20.009084934472494	18.550663006920015
8	27.52735145165655	32.81207716395407	19.806123632427415	19.85444775196196
9	27.496424015154442	14.706962539142538	18.46561255653922	39.331000889163796
10	16.094831252174586	26.227432636177365	32.42065179572428	25.257084315923763
11	36.66070651911274	21.758082443338328	22.1910791088774	19.39013192867153
12	24.711499429764366	24.10163725281735	29.086849786403263	22.10001353101502
13	29.8231029482842	20.21846302561624	24.356694055099084	25.60173997100048
14	24.24025672749768	19.59480358799876	25.79164089081349	30.373298793690072
15	25.826098725806762	27.44929329646745	21.589744581295076	25.134863396430713
16	24.920013532453726	26.566139867575274	23.160794548354357	25.353052051616643
17	23.73259860788863	26.370843000773398	24.940061871616397	24.956496519721576
18	24.317140592681373	25.808509404419013	25.986352477238018	23.887997525661593
19	25.37454812580951	25.375514701618044	25.592994258539697	23.656942914032747
20	25.828806711642923	24.12286636639539	26.29274516247511	23.755581759486574
21	25.86116910229645	24.729374468414132	24.67234980282997	24.737106626459447
22	25.455266006804827	24.211258892669345	26.235307763686976	24.09816733683885
23	25.779792570826526	23.990643455735235	25.454053374832057	24.775510598606186
24	24.777187046882553	24.91251812469792	25.977767037216047	24.33252779120348
25	25.355979390412482	24.601970090964457	25.558981894110026	24.483068624513034
26	24.757107916590133	25.030693825465722	25.36325054862192	24.84894770932222
27	24.940796659481716	25.13314710459418	25.500449462095364	24.42560677382874
28	24.442318100981982	24.94684141343849	25.28512332792082	25.3257171576587
29	24.625437392464285	25.28950064763083	25.494422641947146	24.59063931795774
30	24.80186345008892	24.8985154256553	25.219399984535684	25.080221139720095
31	24.872659791423022	24.54307336922378	25.395552033093953	25.188714806259245
32	24.491827680530452	24.70833856890169	25.99677166800375	24.803062082564107
33	26.18117847200742	25.02803124033405	24.585330961954842	24.205459325703682
34	25.090135807839157	25.018607123870286	26.006476245710697	23.884780822579867
35	24.572257129047852	24.801353310778154	25.824069598840023	24.80231996133398
36	24.699843396554723	25.675231521760146	25.142586470235678	24.482338611449453
37	25.036487178743684	24.30384396052619	26.094856999255754	24.56481186147437
38	24.8318255625145	25.52675326683677	24.891749787365654	24.749671383283076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	4.5
2	8.0
3	8.0
4	22.0
5	36.0
6	36.0
7	42.5
8	49.0
9	52.5
10	56.0
11	56.0
12	75.5
13	95.0
14	117.5
15	140.0
16	140.0
17	260.5
18	381.0
19	381.0
20	507.5
21	634.0
22	700.0
23	766.0
24	766.0
25	821.5
26	877.0
27	877.0
28	1217.5
29	1558.0
30	1929.0
31	2300.0
32	2300.0
33	2964.0
34	3628.0
35	3628.0
36	3964.5
37	4301.0
38	4969.5
39	5638.0
40	5638.0
41	6528.0
42	7418.0
43	8064.5
44	8711.0
45	8711.0
46	8983.5
47	9256.0
48	9256.0
49	9520.5
50	9785.0
51	9644.0
52	9503.0
53	9503.0
54	9480.5
55	9458.0
56	9458.0
57	8953.5
58	8449.0
59	7507.5
60	6566.0
61	6566.0
62	5721.0
63	4876.0
64	4179.0
65	3482.0
66	3482.0
67	2875.0
68	2268.0
69	2268.0
70	1722.0
71	1176.0
72	913.0
73	650.0
74	650.0
75	580.5
76	511.0
77	511.0
78	425.0
79	339.0
80	253.5
81	168.0
82	168.0
83	118.0
84	68.0
85	68.0
86	42.0
87	16.0
88	15.5
89	15.0
90	15.0
91	9.5
92	4.0
93	5.0
94	6.0
95	6.0
96	7.0
97	8.0
98	8.0
99	137.5
100	267.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1227432636177369
2	0.05219004909730545
3	0.012564271078980942
4	0.00579889434414505
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.002899447172072525
12	0.0019329647813816832
13	0.017396683032435148
14	0.0115977886882901
15	0.02899447172072525
16	0.012564271078980942
17	0.027061506939343564
18	0.00579889434414505
19	0.009664823906908416
20	0.00579889434414505
21	0.0038659295627633664
22	0.0115977886882901
23	0.010631306297599258
24	0.017396683032435148
25	0.020296130204507674
26	0.026095024548652725
27	0.010631306297599258
28	0.0038659295627633664
29	0.013530753469671782
30	0.0038659295627633664
31	0.004832411953454208
32	0.008698341516217574
33	0.0115977886882901
34	0.012564271078980942
35	0.017396683032435148
36	0.021262612595198516
37	0.006765376734835891
38	0.0038659295627633664
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	103468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.53303436811382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.52116402116402	21.014226620790968
2	14.552910052910054	10.63323926238064
3	7.804232804232804	8.553369157613949
4	5.126984126984127	7.4921714926354035
5	3.4814814814814814	6.359454130745738
6	2.4523809523809526	5.375575057022461
7	1.7513227513227512	4.4786793984613595
8	1.3597883597883598	3.9741755905207405
9	1.066137566137566	3.5054316310356826
>10	4.843915343915344	27.215177639463413
>50	0.031746031746031744	0.7229288282367495
>100	0.007936507936507936	0.6755711910928983
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	281	0.2715815517841265	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	258	0.24935245679823712	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	160	0.15463718251053465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	9.664823906908416E-4	0.0
14	0.0	0.0	0.0	9.664823906908416E-4	0.0
15	0.0	0.0	0.0	9.664823906908416E-4	0.0
16	0.0	0.0	0.0	0.0019329647813816832	0.0
17	0.0	0.0	0.0	0.0019329647813816832	0.0
18	0.0	0.0	0.0	0.002899447172072525	0.0
19	0.0	0.0	0.0	0.002899447172072525	0.0
20	0.0	0.0	0.0	0.002899447172072525	0.0
21	0.0	0.0	0.0	0.002899447172072525	0.0
22	0.0	0.0	0.0	0.002899447172072525	0.0
23	0.0	0.0	0.0	0.002899447172072525	0.0
24	0.0	0.0	0.0	0.002899447172072525	0.0
25	0.0	0.0	0.0	0.0038659295627633664	0.0
26	0.0	0.0	0.0	0.0038659295627633664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCAATA	20	0.003733997	32.00677	23
GGTATCA	50	2.9522198E-8	28.806093	1
GTATCAA	180	0.0	24.005077	1
CAACACT	35	0.0020571835	22.85093	4
GGGGAGT	50	7.125385E-4	19.204062	20
AACACTG	70	1.7659439E-5	18.280743	5
CTGTAGA	45	0.008786735	17.781538	1
TCCAGCG	45	0.008811269	17.772943	2
GTCTGGA	55	0.0013513813	17.458239	1
AGGACAG	60	0.0024262045	15.99565	5
AACGCAG	285	0.0	15.715025	6
TCAACGC	285	0.0	15.715025	4
CAACGCA	290	0.0	15.444076	5
TATCAAC	295	0.0	15.182312	2
ATCAACG	295	0.0	15.182312	3
GGCAGGT	65	0.0041218307	14.772355	19
CTTCAAG	65	0.0041218307	14.772355	1
CAGGTCC	70	0.0067393733	13.717187	21
TGGGGAG	70	0.0067393733	13.717187	19
AGTACAT	275	0.0	13.384648	13
>>END_MODULE