FastQCFastQC Report
Thu 2 Feb 2017
SRR4062827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103468
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2480.23968763289132872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT1510.1459388409943171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCA200.003739810831.99661611
CACATGT401.581061E-423.99746319
GGTATCA401.581061E-423.9974631
GTATCAA1100.023.2702661
ACTGTGC350.002049422422.8657788
ACAGAGT400.00444668720.0075553
TTCTGTA450.00880283617.77589815
TATCAAC1601.8189894E-1216.9982032
CAACGCA1807.2759576E-1215.116825
AACGCAG1807.2759576E-1215.116826
ATCAACG1851.0913936E-1114.7082563
TCAACGC1851.0913936E-1114.7082564
AGAGTAC2202.0190782E-1012.36232911
ACGCAGA2252.928573E-1012.0934567
CGCAGAG2304.2200554E-1011.8305558
CAGAGTA2304.2564352E-1011.82483710
AGTACAT1907.941619E-610.10419513
GCAGAGT2705.979018E-910.077889
GAGTACA2153.4320772E-58.92928812
CATGGGG2204.3083332E-47.99915417
Produced by FastQC (version 0.11.5)