##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	103468
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254890400896894	32.0	32.0	32.0	32.0	32.0
2	31.386254687439596	32.0	32.0	32.0	32.0	32.0
3	31.420361851007076	32.0	32.0	32.0	32.0	32.0
4	31.492016855452892	32.0	32.0	32.0	32.0	32.0
5	31.430925503537324	32.0	32.0	32.0	32.0	32.0
6	34.9355066300692	36.0	36.0	36.0	36.0	36.0
7	34.98367611242123	36.0	36.0	36.0	36.0	36.0
8	34.90282019561604	36.0	36.0	36.0	36.0	36.0
9	34.98254532802412	36.0	36.0	36.0	36.0	36.0
10	34.861918660842	36.0	36.0	36.0	32.0	36.0
11	35.01630455793095	36.0	36.0	36.0	36.0	36.0
12	34.964462442494295	36.0	36.0	36.0	36.0	36.0
13	34.992364789113545	36.0	36.0	36.0	36.0	36.0
14	34.92736884833958	36.0	36.0	36.0	32.0	36.0
15	34.906492828700664	36.0	36.0	36.0	32.0	36.0
16	34.944997487145784	36.0	36.0	36.0	36.0	36.0
17	34.910687362276256	36.0	36.0	36.0	32.0	36.0
18	34.88033015038466	36.0	36.0	36.0	32.0	36.0
19	34.87319751034136	36.0	36.0	36.0	32.0	36.0
20	34.87865813584877	36.0	36.0	36.0	32.0	36.0
21	34.891695983299186	36.0	36.0	36.0	32.0	36.0
22	34.83889705029574	36.0	36.0	36.0	32.0	36.0
23	34.79614953415549	36.0	36.0	36.0	32.0	36.0
24	34.77916843855105	36.0	36.0	36.0	32.0	36.0
25	34.784474426875946	36.0	36.0	36.0	32.0	36.0
26	34.709494723006145	36.0	36.0	36.0	32.0	36.0
27	34.68810646770016	36.0	36.0	36.0	32.0	36.0
28	34.670613136428656	36.0	36.0	36.0	32.0	36.0
29	34.64102911044961	36.0	36.0	36.0	32.0	36.0
30	34.63644798391773	36.0	36.0	36.0	32.0	36.0
31	34.60332663238876	36.0	36.0	36.0	32.0	36.0
32	34.58582363629335	36.0	36.0	36.0	32.0	36.0
33	34.55207407121042	36.0	36.0	36.0	32.0	36.0
34	34.524654965786524	36.0	36.0	36.0	32.0	36.0
35	34.527970000386595	36.0	36.0	36.0	32.0	36.0
36	34.51434259867785	36.0	36.0	36.0	32.0	36.0
37	34.49022886303012	36.0	36.0	36.0	32.0	36.0
38	34.12275292844164	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	3.0
22	8.0
23	28.0
24	87.0
25	166.0
26	391.0
27	706.0
28	1220.0
29	1962.0
30	2880.0
31	3920.0
32	5642.0
33	8475.0
34	21118.0
35	56861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.955189545516056	18.5215256432562	12.552920025517603	26.970364785710142
2	16.084199945876986	21.281942243012335	36.40545869254262	26.228399118568056
3	17.60157729926161	23.271929485444776	29.773456527622066	29.35303668767155
4	13.095836393860905	16.519117021687865	34.869718173734874	35.51532841071636
5	14.57165500444582	36.31654231259907	33.4847489078749	15.627053775080219
6	34.431901650751925	35.35005992190822	16.437932500869834	13.780105926470021
7	29.976417829667145	31.050179765724668	20.185951211968916	18.78745119263927
8	27.23722998115305	32.97153626830329	19.89754989610013	19.89368385444353
9	27.455756275311472	14.374498603338456	18.332511767719236	39.837233353630836
10	15.768312602085688	26.87136961543294	32.28179032928373	25.078527453197637
11	36.51599060570037	21.619453545574917	22.230276319986082	19.63427952873863
12	24.06831097537403	24.293501372404993	29.428422314145436	22.20976533807554
13	29.464182162601	20.18015231762477	24.6308037267561	25.724861793018132
14	23.948467158928363	19.549039316503652	25.71616345150191	30.786330073066072
15	25.800247419492017	27.752541848687518	21.19205938067808	25.25515135114238
16	24.74388216646693	26.912668651177174	22.99068310975374	25.352766072602158
17	23.500985812038504	26.835350059921907	24.97583794023273	24.68782618780686
18	24.2084509220242	25.842772644682416	25.8862643522635	24.062512081029883
19	25.04832411953454	25.922990683109752	25.24548652723547	23.783198670120232
20	25.940387366142186	24.382417752348555	25.83214133838481	23.845053543124443
21	25.637878377855955	24.884022113117098	24.304132678702594	25.17396683032435
22	25.11307843971083	24.810569451424595	25.757722194301618	24.318629914562955
23	25.781884254068892	23.93107047589593	25.452313758843314	24.834731511191865
24	24.74871457842038	25.108246027757374	25.419453357559824	24.723586036262418
25	25.046391154753163	24.615340008505047	25.607917423744542	24.730351412997255
26	24.766111261452817	25.177832759887114	25.067653767348357	24.988402211311712
27	24.764178296671435	25.547029033131018	25.154637182510537	24.534155487687016
28	24.40271388255306	25.074419144083194	24.958441257200295	25.564425716163452
29	24.325395291297795	25.564425716163452	25.2319557737658	24.878223218772956
30	24.68685970541617	24.96134070437237	25.207793713998534	25.144005876212937
31	24.507093980747673	25.081184520818027	25.10148065102254	25.31024084741176
32	24.477133026636256	24.708122318011366	25.707465109985694	25.107279545366683
33	26.025437816522984	25.365330343681137	24.264506900684268	24.344724939111607
34	24.908184172884372	25.038659295627635	25.89302972899834	24.16012680248966
35	24.864692465303282	24.648200409788533	25.79058259558511	24.696524529323074
36	24.46070282599451	25.89206324660765	24.801871109908376	24.845362817489466
37	24.567015888970502	24.63177020914679	26.04766691150887	24.753546990373835
38	24.644334480225773	25.349866625430085	24.883055630726407	25.122743263617735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	4.0
2	7.0
3	7.0
4	20.0
5	33.0
6	33.0
7	38.5
8	44.0
9	44.5
10	45.0
11	45.0
12	63.0
13	81.0
14	104.5
15	128.0
16	128.0
17	247.5
18	367.0
19	367.0
20	502.0
21	637.0
22	661.0
23	685.0
24	685.0
25	786.5
26	888.0
27	888.0
28	1203.5
29	1519.0
30	1997.0
31	2475.0
32	2475.0
33	3079.0
34	3683.0
35	3683.0
36	3901.0
37	4119.0
38	4805.0
39	5491.0
40	5491.0
41	6565.5
42	7640.0
43	8181.0
44	8722.0
45	8722.0
46	9169.0
47	9616.0
48	9616.0
49	9654.5
50	9693.0
51	9557.5
52	9422.0
53	9422.0
54	9546.5
55	9671.0
56	9671.0
57	9098.0
58	8525.0
59	7604.0
60	6683.0
61	6683.0
62	5699.0
63	4715.0
64	4092.5
65	3470.0
66	3470.0
67	2884.0
68	2298.0
69	2298.0
70	1704.0
71	1110.0
72	862.0
73	614.0
74	614.0
75	563.0
76	512.0
77	512.0
78	411.5
79	311.0
80	247.0
81	183.0
82	183.0
83	118.5
84	54.0
85	54.0
86	34.5
87	15.0
88	12.0
89	9.0
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009664823906908416
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.002899447172072525
9	0.006765376734835891
10	9.664823906908416E-4
11	9.664823906908416E-4
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	103468.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.84265666679553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.61429641341433	19.02907179031198
2	15.211783306981777	10.600378861097152
3	8.543452331419378	8.930297289983377
4	5.456159329838284	7.604283449955542
5	3.60600260741727	6.28213553949047
6	2.6157388144572966	5.468357366528782
7	1.9416937116862223	4.735763714385124
8	1.583867299104047	4.414891560675764
9	1.092896174863388	3.427146557389724
>10	5.295276136584284	28.35466037808791
>50	0.030512329755069206	0.6678393319673716
>100	0.008321544478655237	0.4851741601268025
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	248	0.23968763289132872	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	151	0.1459388409943171	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	9.664823906908416E-4	0.0
14	0.0	0.0	0.0	9.664823906908416E-4	0.0
15	0.0	0.0	0.0	9.664823906908416E-4	0.0
16	0.0	0.0	0.0	0.0019329647813816832	0.0
17	0.0	0.0	0.0	0.0019329647813816832	0.0
18	0.0	0.0	0.0	0.002899447172072525	0.0
19	0.0	0.0	0.0	0.002899447172072525	0.0
20	0.0	0.0	0.0	0.002899447172072525	0.0
21	0.0	0.0	0.0	0.002899447172072525	0.0
22	0.0	0.0	0.0	0.002899447172072525	0.0
23	0.0	0.0	0.0	0.002899447172072525	0.0
24	0.0	0.0	0.0	0.002899447172072525	0.0
25	0.0	0.0	0.0	0.0038659295627633664	0.0
26	0.0	0.0	0.0	0.0038659295627633664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCTCA	20	0.0037398108	31.996616	11
CACATGT	40	1.581061E-4	23.997463	19
GGTATCA	40	1.581061E-4	23.997463	1
GTATCAA	110	0.0	23.270266	1
ACTGTGC	35	0.0020494224	22.865778	8
ACAGAGT	40	0.004446687	20.007555	3
TTCTGTA	45	0.008802836	17.775898	15
TATCAAC	160	1.8189894E-12	16.998203	2
CAACGCA	180	7.2759576E-12	15.11682	5
AACGCAG	180	7.2759576E-12	15.11682	6
ATCAACG	185	1.0913936E-11	14.708256	3
TCAACGC	185	1.0913936E-11	14.708256	4
AGAGTAC	220	2.0190782E-10	12.362329	11
ACGCAGA	225	2.928573E-10	12.093456	7
CGCAGAG	230	4.2200554E-10	11.830555	8
CAGAGTA	230	4.2564352E-10	11.824837	10
AGTACAT	190	7.941619E-6	10.104195	13
GCAGAGT	270	5.979018E-9	10.07788	9
GAGTACA	215	3.4320772E-5	8.929288	12
CATGGGG	220	4.3083332E-4	7.999154	17
>>END_MODULE