FastQCFastQC Report
Thu 2 Feb 2017
SRR4062826_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062826_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830071
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT112850.39875324682666974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT76350.26978121750302375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT41000.14487269047313653No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA34680.12254109525874086No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG34300.12119837276167275No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA33850.1196083066467237No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG33200.11731154448068616No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32970.11649884402193443No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA32600.11519145632742077No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG32470.11473210389421325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31960.11293002896393764No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC31800.11236467212306687No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31650.11183465008475053No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG31130.1099972403519205No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC28700.10141088333119558No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT28530.10081019168777038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC6050.016.1309227
TAGGACG12250.016.0620164
ACCGTCG6200.015.7409358
CTAGCGG8000.015.20010929
TCTAGCG8000.015.19903528
GATATAC5800.015.1886271
AGGACGT13150.014.8410675
GTATTAC2700.014.83064651
ATACCGT6850.014.7125766
TAGCGTA6000.014.6654777
CGTCGTA6800.014.58808310
AAACGCT9000.014.57788329
CGCTTCG9050.014.49734132
TAGCGGC8400.014.476294530
GGACGTG13400.014.4450576
AATAGCG6250.014.3330645
ACGTATT901.5353436E-414.22232429
CGAGCCG9000.014.22207315
CGGTCCA9150.014.16378610
ACGCTTC9100.014.06603431
Produced by FastQC (version 0.11.5)