##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062826_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2830071
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210300377623035	32.0	32.0	32.0	32.0	32.0
2	30.902139204281447	32.0	32.0	32.0	32.0	32.0
3	30.953354527147905	32.0	32.0	32.0	32.0	32.0
4	31.00106181081676	32.0	32.0	32.0	32.0	32.0
5	30.962933085424357	32.0	32.0	32.0	32.0	32.0
6	34.58882409663927	36.0	36.0	36.0	32.0	36.0
7	34.50589366839206	36.0	36.0	36.0	32.0	36.0
8	34.495786854817425	36.0	36.0	36.0	32.0	36.0
9	34.58912232237283	36.0	36.0	36.0	32.0	36.0
10	34.36974160718936	36.0	36.0	36.0	32.0	36.0
11	34.5956564340612	36.0	36.0	36.0	32.0	36.0
12	34.44701458019958	36.0	36.0	36.0	32.0	36.0
13	34.51344895587425	36.0	36.0	36.0	32.0	36.0
14	34.42326287927052	36.0	36.0	36.0	32.0	36.0
15	34.386805843386966	36.0	36.0	36.0	32.0	36.0
16	34.39788189059568	36.0	36.0	36.0	32.0	36.0
17	34.325200321829385	36.0	36.0	36.0	32.0	36.0
18	34.35280210284477	36.0	36.0	36.0	32.0	36.0
19	34.335031877292124	36.0	36.0	36.0	32.0	36.0
20	34.304945706309134	36.0	36.0	36.0	32.0	36.0
21	34.27583830935691	36.0	36.0	36.0	32.0	36.0
22	34.266739244351115	36.0	36.0	36.0	32.0	36.0
23	34.220363022694485	36.0	36.0	36.0	32.0	36.0
24	34.20766263461235	36.0	36.0	36.0	32.0	36.0
25	34.18252792951131	36.0	36.0	36.0	32.0	36.0
26	34.13750114396423	36.0	36.0	36.0	32.0	36.0
27	34.14369250806782	36.0	36.0	36.0	32.0	36.0
28	34.12624983613485	36.0	36.0	36.0	32.0	36.0
29	34.087695679719694	36.0	36.0	36.0	32.0	36.0
30	34.06064830175639	36.0	36.0	36.0	32.0	36.0
31	34.07801465051583	36.0	36.0	36.0	32.0	36.0
32	34.02754312524315	36.0	36.0	36.0	32.0	36.0
33	33.98802538876233	36.0	36.0	36.0	32.0	36.0
34	33.98169197875248	36.0	36.0	36.0	32.0	36.0
35	33.939867586360904	36.0	36.0	36.0	32.0	36.0
36	33.896172922870136	36.0	36.0	36.0	32.0	36.0
37	33.88719328949698	36.0	36.0	36.0	32.0	36.0
38	33.28140919432764	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	7.0
8	8.0
9	13.0
10	19.0
11	27.0
12	13.0
13	18.0
14	506.0
15	954.0
16	1277.0
17	1588.0
18	1941.0
19	2431.0
20	3212.0
21	4199.0
22	6152.0
23	8736.0
24	12187.0
25	17958.0
26	25547.0
27	36882.0
28	51669.0
29	70365.0
30	94907.0
31	128247.0
32	174777.0
33	264077.0
34	595580.0
35	1326773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38606536265888	17.489619920695347	11.658024855584582	25.466289861061185
2	16.257894176755315	20.120423869961172	38.36484168867768	25.25684026460584
3	18.639941535388687	24.87159624927467	28.61101314393589	27.877449071400758
4	12.345516293161356	15.833195229242778	36.33262437937082	35.48866409822505
5	14.322240517868877	36.85314092280729	33.52013738224631	15.304481177077516
6	33.21012074621528	35.97065512206774	17.35809881600018	13.461125315716796
7	29.639574413504118	30.862052577479503	21.2909146095628	18.207458399453582
8	27.863390592547	33.039369566799785	19.758633580843334	19.338606259809886
9	27.77389486598183	14.366671566438075	18.786960294890857	39.07247327268924
10	15.988598073248228	26.67855810219473	31.533104738859553	25.79973908569749
11	36.97761171615477	21.352950717083033	22.82842409930435	18.841013467457852
12	25.396141115742854	23.846665128380355	28.83520041950693	21.92199333636986
13	29.47951119336657	19.823286627013	25.457553839374032	25.239648340246397
14	23.71788325153112	20.387710337823297	25.42256624424472	30.471840166400867
15	24.814870317328815	27.70381936884845	22.989975146393114	24.491335167429618
16	25.161320873401454	25.84063547348825	24.651172323911112	24.346871329199185
17	23.467576622935987	26.08876668174621	25.854126611626327	24.58953008369147
18	24.434926230504583	24.71698606591903	27.511364375227927	23.336723328348462
19	25.417592657705303	25.018525710364255	26.055788803014718	23.50809282891573
20	25.623149668291934	24.333668563319243	25.828810791060313	24.21437097732851
21	26.786935946219476	24.291134015955492	24.81861370408273	24.103316333742303
22	25.536071953082928	24.70291648585576	25.456032205308844	24.304979355752472
23	23.64682060089477	24.59035684752878	26.3127168896522	25.450105661924248
24	24.481289088190486	25.484378152985926	25.923406318570542	24.110926440253046
25	24.592590288890523	24.815379221822006	26.03350881260284	24.55852167668463
26	23.858581674681023	25.82330625911378	26.6166249514933	23.7014871147119
27	24.72395595168849	25.271096705705908	25.58959955502187	24.415347787583723
28	23.702812686844588	25.243542863119867	26.377685084801822	24.675959365233727
29	23.878044177816665	25.253255945940023	26.253098334620844	24.615601541622464
30	23.751552575552786	25.503052147103773	26.59638336027703	24.149011917066403
31	24.573693978427446	25.270752382069706	25.30393386037347	24.85161977912938
32	23.88968928155835	25.654122234272286	25.260286635986716	25.19590184818265
33	23.512761289811888	25.261042445282506	26.134458592243714	25.09173767266189
34	24.491558546657874	25.362561604904503	26.248831158917863	23.897048689519764
35	25.1619294126295	25.161788051291552	26.14318914005657	23.533093396022377
36	23.749955383001154	26.02151723241021	25.696528779819616	24.53199860476902
37	24.781467110517426	25.758539533108582	25.437878562997827	24.022114793376165
38	23.948211487780107	25.85354787140437	25.78004688514835	24.418193755667172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1754.0
1	1328.0
2	902.0
3	902.0
4	2059.5
5	3217.0
6	3217.0
7	3635.5
8	4054.0
9	3845.5
10	3637.0
11	3637.0
12	4775.0
13	5913.0
14	7323.5
15	8734.0
16	8734.0
17	13058.5
18	17383.0
19	17383.0
20	20461.0
21	23539.0
22	24025.0
23	24511.0
24	24511.0
25	28913.5
26	33316.0
27	33316.0
28	43017.0
29	52718.0
30	64918.0
31	77118.0
32	77118.0
33	96011.5
34	114905.0
35	114905.0
36	124864.0
37	134823.0
38	150847.0
39	166871.0
40	166871.0
41	173436.5
42	180002.0
43	199725.5
44	219449.0
45	219449.0
46	224644.5
47	229840.0
48	229840.0
49	241767.5
50	253695.0
51	252170.5
52	250646.0
53	250646.0
54	238214.5
55	225783.0
56	225783.0
57	219665.5
58	213548.0
59	191757.0
60	169966.0
61	169966.0
62	160698.5
63	151431.0
64	124297.5
65	97164.0
66	97164.0
67	80864.5
68	64565.0
69	64565.0
70	51633.0
71	38701.0
72	30205.0
73	21709.0
74	21709.0
75	16269.5
76	10830.0
77	10830.0
78	10727.0
79	10624.0
80	8322.0
81	6020.0
82	6020.0
83	6131.5
84	6243.0
85	6243.0
86	4139.5
87	2036.0
88	1796.5
89	1557.0
90	1557.0
91	1131.5
92	706.0
93	585.5
94	465.0
95	465.0
96	420.5
97	376.0
98	376.0
99	848.0
100	1320.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1237778133481457
2	0.05519296159000958
3	0.011483810830187654
4	0.002826784204353884
5	3.88682828098659E-4
6	1.0600440766327064E-4
7	0.0
8	6.006916434252003E-4
9	2.4734361788096484E-4
10	8.127004587517415E-4
11	0.0022614273634831068
12	0.0028974538094627307
13	0.014275260231987111
14	0.009823075110129746
15	0.02484036619575975
16	0.01084778438420803
17	0.022296260411841257
18	0.006183590447024121
19	0.00777365656197318
20	0.006112920841915274
21	0.007031625708330286
22	0.008021000179854146
23	0.010282427543337252
24	0.014557938652422501
25	0.017208048844004266
26	0.019964163443249302
27	0.009505061887139933
28	0.006324929657241815
29	0.011483810830187654
30	0.0033921410452246604
31	0.006324929657241815
32	0.007420308536428944
33	0.009293053071813393
34	0.012190506881276124
35	0.01565331753160963
36	0.015264634703510973
37	0.010282427543337252
38	0.006254260052132968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2830071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.59027135310666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90332330048093	32.50778968511708
2	14.675741109991424	13.087905568049207
3	5.445310510044635	7.284236197344416
4	2.4294143362273966	4.333129779260284
5	1.3282351883361707	2.9613183734327286
6	0.7512032731733087	2.0097814675283843
7	0.4978724206932822	1.554018643655907
8	0.334871181404759	1.1945597477738887
9	0.24383973218887328	0.9785591842473644
>10	1.184408334300252	9.377783089598713
>50	0.08808966109426629	2.7625147788827085
>100	0.09126703056189907	8.692809834339013
>500	0.01666373428104143	5.131118786798094
>1k	0.009601484990496399	7.455030816404299
>5k	7.935111562393718E-5	0.2701482718383207
>10k+	7.935111562393718E-5	0.3992957757295938
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	11285	0.39875324682666974	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7635	0.26978121750302375	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4100	0.14487269047313653	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3468	0.12254109525874086	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3430	0.12119837276167275	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3385	0.1196083066467237	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3320	0.11731154448068616	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3297	0.11649884402193443	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3260	0.11519145632742077	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3247	0.11473210389421325	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3196	0.11293002896393764	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3180	0.11236467212306687	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3165	0.11183465008475053	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3113	0.1099972403519205	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2870	0.10141088333119558	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2853	0.10081019168777038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.066960510884709E-5
2	0.0	0.0	0.0	0.0	7.066960510884709E-5
3	0.0	0.0	0.0	0.0	7.066960510884709E-5
4	0.0	0.0	0.0	0.0	7.066960510884709E-5
5	0.0	0.0	0.0	0.0	1.0600440766327064E-4
6	0.0	0.0	0.0	0.0	1.7667401277211774E-4
7	0.0	0.0	0.0	0.0	1.7667401277211774E-4
8	0.0	3.533480255442354E-5	0.0	0.0	1.7667401277211774E-4
9	0.0	3.533480255442354E-5	0.0	3.533480255442354E-5	1.7667401277211774E-4
10	0.0	3.533480255442354E-5	0.0	3.533480255442354E-5	1.7667401277211774E-4
11	0.0	3.533480255442354E-5	0.0	3.533480255442354E-5	1.7667401277211774E-4
12	0.0	3.533480255442354E-5	0.0	3.533480255442354E-5	2.4734361788096484E-4
13	0.0	3.533480255442354E-5	0.0	7.066960510884709E-5	3.180132229898119E-4
14	0.0	3.533480255442354E-5	0.0	1.0600440766327064E-4	3.180132229898119E-4
15	0.0	3.533480255442354E-5	0.0	1.0600440766327064E-4	4.593524332075061E-4
16	0.0	3.533480255442354E-5	0.0	1.0600440766327064E-4	4.593524332075061E-4
17	0.0	3.533480255442354E-5	0.0	1.4133921021769417E-4	4.946872357619297E-4
18	3.533480255442354E-5	7.066960510884709E-5	0.0	2.4734361788096484E-4	4.946872357619297E-4
19	3.533480255442354E-5	7.066960510884709E-5	0.0	3.180132229898119E-4	4.946872357619297E-4
20	3.533480255442354E-5	7.066960510884709E-5	0.0	4.2401763065308255E-4	5.653568408707767E-4
21	3.533480255442354E-5	1.0600440766327064E-4	0.0	5.300220383163532E-4	6.360264459796238E-4
22	3.533480255442354E-5	1.0600440766327064E-4	0.0	0.0011307136817415534	7.06696051088471E-4
23	3.533480255442354E-5	1.0600440766327064E-4	0.0	0.0025441057839184953	7.77365656197318E-4
24	3.533480255442354E-5	1.0600440766327064E-4	0.0	0.00388682828098659	7.77365656197318E-4
25	3.533480255442354E-5	1.0600440766327064E-4	0.0	0.004840867949956026	7.77365656197318E-4
26	3.533480255442354E-5	1.0600440766327064E-4	0.0	0.006042251236806426	8.833700638605887E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	605	0.0	16.130922	7
TAGGACG	1225	0.0	16.062016	4
ACCGTCG	620	0.0	15.740935	8
CTAGCGG	800	0.0	15.200109	29
TCTAGCG	800	0.0	15.199035	28
GATATAC	580	0.0	15.188627	1
AGGACGT	1315	0.0	14.841067	5
GTATTAC	270	0.0	14.8306465	1
ATACCGT	685	0.0	14.712576	6
TAGCGTA	600	0.0	14.665477	7
CGTCGTA	680	0.0	14.588083	10
AAACGCT	900	0.0	14.577883	29
CGCTTCG	905	0.0	14.497341	32
TAGCGGC	840	0.0	14.4762945	30
GGACGTG	1340	0.0	14.445057	6
AATAGCG	625	0.0	14.333064	5
ACGTATT	90	1.5353436E-4	14.222324	29
CGAGCCG	900	0.0	14.222073	15
CGGTCCA	915	0.0	14.163786	10
ACGCTTC	910	0.0	14.066034	31
>>END_MODULE