FastQCFastQC Report
Thu 2 Feb 2017
SRR4062826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830071
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT133200.47065957002492165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT93460.33023906467364245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT54710.19331670477525123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40360.14261126310965344No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG36330.12837133768022074No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA35210.12441383979412529No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC34910.1233537957174926No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA33760.1192902934237339No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA33310.11770022730878482No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA33210.11734687928324061No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC33030.11671085283726099No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG32260.11399007304057036No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32100.11342471619969959No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG31640.11179931528219611No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC31460.11116328883621648No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31360.11080994081067225No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG30320.10713512134501219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG7300.017.53406328
CTAGCGG7150.017.45436129
TAGCGGC7500.016.42649530
AACCGCG4850.016.165357
TAAACGC8750.015.54269428
ATCGTTT6900.015.53606929
ACCGTCG6100.015.4757948
CGCGGTC5300.015.39606510
CATCGTT7000.015.31412528
AAACGCT8750.015.17698429
GTATTAC2250.014.9344961
CGCTTCG8850.014.82470332
GTCCTAT9500.014.8222071
TAACGCA650.00416289114.7695984
TACCGTC6500.014.7695987
ACGCTTC9000.014.75540131
TATACAC7650.014.6408863
CGTTTAT7550.014.41044431
GTAAACG9400.014.29772327
CGGCATC7500.014.29318325
Produced by FastQC (version 0.11.5)